Anti nf κb p65

Manufactured by ABclonal

The Anti-NF-κB p65 is a primary antibody that binds to the NF-κB p65 subunit. NF-κB p65 is a critical transcription factor involved in regulating the expression of genes related to immune response, inflammation, and cell survival.

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Lab products found in correlation

Anti irf3, by ABclonal (1 mentions) Anti p irf3, by ABclonal (1 mentions) Anti ubiquitin, by LifeSensors (1 mentions) Anti flag, by Merck Group (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti c ebpβ, by Abcam (1 mentions) Anti il 1β, by ABclonal (1 mentions) Bca protein quantitation kit, by Keygen Biotech (1 mentions) Whole cell lysis kit, by Keygen Biotech (1 mentions) Anti β actin, by ABclonal (1 mentions)

Spelling variants of this product

NF-κB (16 citations) NF-κB p65 (12 citations) Anti-p65 (9 citations) Anti‐NF‐κB (5 citations)

2 protocols using anti nf κb p65

MERS-CoV Interaction Mechanism Study

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The HA-tagged MERS-CoV viral genes were acknowledged to Wenjie Tan, Chinese Center for Disease Control and Prevention. Flag-tagged UBR5, the C2768A mutant, and MYC-tagged different domains of UBR5 were kindly provided by Guoqiang Xu, Soochow University. Green fluorescent protein (GFP)-tagged UBR5 was kindly provided by Yong Tae Kwon, Seoul National University. All of the other genes with different tags or mutants were subcloned into pCAGGS vector. Lentiviral shRNA clones targeting human UBR5 and the nontargeting control plasmid were purchased from Tsingke. The following antibodies were used in immunoblotting: anti-Flag (Sigma), anti-HA (CST), anti-V5 (CST), anti-GFP (CST), anti-GST (CST), anti-ubiquitin (LifeSensors), anti-UBR5 (CST), anti-IRF3 (Abclonal), anti-p-IRF3 (Abclonal), anti-NF-κB p65 (Abclonal), anti-β-actin (Sigma), and anti-histone (CST).

Zhou Y., Zheng R., Liu D., Liu S., Disoma C., Li S., Liao Y., Chen Z., Du A., Dong Z., Zhang Y., Liu P., Razzaq A., Chen D., Chen X., Zhong X., Liu S., Tao S., Liu Y., Xu L., Deng X., Li J., Jiang T., Zhao J., Li S, & Xia Z. (2022). UBR5 Acts as an Antiviral Host Factor against MERS-CoV via Promoting Ubiquitination and Degradation of ORF4b. Journal of Virology, 96(17), e00741-22.

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Protein extraction and Western blot analysis

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The Whole Cell Lysis Kit and the BCA Protein Quantitation Kit (KeyGEN Bio TECH) were used to achieve cell protein extraction and quantification. Then Western blotting (WB) was performed according to a previously described standard method17 (link) using anti-C/EBPβ (1:1000, Cat#ab32358, Abcam), anti-iNOS (1:1000, Cat#OM641716, Omnimabs), anti-NOD2 (1:1000, Cat#A15992, ABclonal), anti-NOD1 (1:1000, Cat#DF6378, Affinity), anti-phospho-RIPK2 (1:500, Cat#AF0049, Affinity), anti-RIPK2 (1:1000, Cat#DF6967, Affinity), anti-phospho-NF-κB p65 (1:500, Cat#AP0123, ABclonal), anti-NF-κB p65 (1:1000, Cat#A16271, ABclonal), anti-IL-1β (1:1000, Cat#A1112, ABclonal), and anti-β-actin (1:20000, cat#AC026, ABclonal) antibodies. The experiments were independently performed in triplicate.

Luo Y., Ge P., Wen H., Zhang Y., Liu J., Dong X., Lan B., Zhang G., Yang Q, & Chen H. (2022). C/EBPβ Promotes LPS-Induced IL-1β Transcription and Secretion in Alveolar Macrophages via NOD2 Signaling. Journal of Inflammation Research, 15, 5247-5263.

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