The QIAsure Methylation Test was performed according to the manufacturer's instructions. The QIAsure Methylation Test is designed and manufactured by Self‐screen BV (Amsterdam, the Netherlands), and under an exclusive license distributed by QIAGEN (Hilden, Germany). Sample input in the assay is 2.5 µL bisulfite‐converted DNA. The assay was performed on a Rotor‐Gene Q MDx 5plex HRM instrument. AssayManager software (QIAGEN) controls amplification as well as data analysis and reporting using a fixed assay profile. The AssayManager software calculates ΔΔCt values for both FAM19A4 and hsa‐mir124‐2. A sample is considered “Hypermethylation‐positive” when the ΔΔCt for at least one of the targets is below its cutoff, and “Hypermethylation‐negative” when both targets are above their cutoff. A sample is considered invalid when the housekeeping gene (ACTB) Ct value is above its cutoff.
Assaymanager software
Manufactured by Qiagen
The AssayManager software is a laboratory informatics solution developed by Qiagen. The software is designed to provide centralized control and monitoring of automated liquid handling instruments, enabling efficient and reliable management of laboratory workflows.
Automatically generated - may contain errors
2 protocols using assaymanager software
1
QIAsure Methylation Test Protocol
2
Quantitative Assessment of FAM19A4/miR124-2 Methylation
Check if the same lab product or an alternative is used in the 5 most similar protocols
FAM19A4/miR124‐2 methylation analysis including sample DNA extraction was performed as previously described.24 For bisulfite‐conversion, the EZ DNA Methylation Kit was used according to the manufacturer's specifications (Zymo Research, Irvine, California).32 Bisulfite‐converted DNA was subsequently used as input for quantitative PCR analysis of the FAM19A4 and miR124‐2 promoter methylation levels using the QIAsure Methylation Test (QIAGEN). For all centers, a sample input of 2.5 μL bisulfite‐converted DNA was used for PCR on the Rotor‐Gene Q MDx 5plex HRM instrument (QIAGEN) equipped with the AssayManager software (QIAGEN). This software runs the assay followed by automatic quality assurance and data analysis. The reported results were hypermethylation‐positive, negative or invalid. Additional quality assurance was employed using the housekeeping gene β‐actin (ACTB) as a reference for successful bisulfite‐conversion, sample quality and signal normalization. Methylation testing was performed by local technicians blinded for the clinical data.
+ Expand
FAM19A4/miR124‐2 methylation analysis including sample DNA extraction was performed as previously described.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!