Total proteins were isolated from the aortic media (VSMCs were the only cell type in this layer) or cultured VSMCs, and the procedure of western blot analysis was performed as described in our previous reports [8 –10 (link)]. The expressions of key enzymes in mevalonate pathway were detected using their specific antibodies: HMGR (ab174830, Abcam, UK), P-HMGR (09-356, Millipore, USA), FPPS (ab38854, Abcam, UK), SQS (sc-136372, Santa Cruz, Japan), GGPPS (ab167168, Abcam, UK), FNT-β (ab74206, Abcam, UK), or GGT-1β (sc-18996, Santa Cruz, Japan). To ensure equal protein loading, β-actin was used as an endogenous control (ab8226, Abcam, UK).
Ab38854
Manufactured by Abcam
Sourced in United Kingdom, United States
Ab38854 is a recombinant protein that can be used for research purposes. The core function of this product is to serve as a tool for scientific investigation.
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Lab products found in correlation
2 protocols using ab38854
1
Western Blot Analysis of Mevalonate Pathway Enzymes
2
Immunohistochemical Analysis of FDPS in Pancreatic Cancer
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Tissue arrays comprised of normal pancreas (from donors for pancreas transplant) and PC patient samples were obtained from the Rapid Autopsy Program of the University of Nebraska Medical Center (PI: Michael Hollingsworth, IRB# 091-01-FB). The tissues were stained with anti-FDPS polyclonal antibody (ab38854, Abcam, Cambridge, MA, USA) using an antibody dilution of 1 : 100. The immunostaining technique was performed according to the standard laboratory procedure (Moniaux et al, 2008 (link)). The FDPS staining was scored by a pathologist using the criteria described in Supplementary Methods . Representative photographs were taken under a Nikon light microscope with a Q-capture Micropublisher 5.0 camera (Leeds Precision Instruments, Minneapolis, MN, USA) by utilising the Q-capture suite software package (QImaging, Surrey, BC, Canada).
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