Ab115183

CAG (containing per 100 g ammonium glycyrrhizin 2.8 g, glycine and methionine 2 g each) was made in our laboratory; Florfenicol was purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China); LPS, HEPES and collagenase type IV were purchased from Sigma-Aldrich (St. Louis, MO, USA); FBS was purchased from Gibco (Los Angeles, CA, USA); Annexin V-FITC/PI apoptosis detection kit was purchased from Vazyme (Nanjing, China); Commercial kits for ALT, AST, measurement were purchased from Angle Gene Biotech (Nanjing, China). Polyclonal caspase-3 (ab115183, Abcam, Cambridge, UK), caspase-9 (ab115161, Abcam, Cambridge, UK), bax (orb334986, biorbyt, Cambridge, UK), bcl-2 (610538, BD Biosciences, San Jose, CA, USA), cytochrome c (H-104) (sc-715, SantaCruz Biotechnology, Dallas, TX, USA) and β-actin (A5441, Sigma, Saint Louis, MO, USA) were used for the western blot assays. The cells (Laboratory of Veterinary Pharmacology and Toxicology, Nanjing, China) were isolated from rooster.