Upon reaching confluence, cells were fixed in 4% paraformaldehyde, and then stained with YAP1 primary antibody (Novus Biologics, Cat. No. NB110-58358), and AlexaFluor 488 Goat anti-Rabbit secondary (Jackson ImmunoResearch, Cat. No. 111-545-144). The nuclei were then stained with DAPI.
Analysis of YAP localization was performed as in ref. 29 (link). Briefly, the nucleus of each cell was segmented using the DAPI channel, and the average intensity of YAP was measured inside the nucleus and just outside the nucleus. The nuclear YAP score was then obtained by taking the ratio of nuclear to cytoplasmic YAP mean intensity.
Statistical analysis was done in Prism using Ordinary one-way ANOVA with Tukey's test for multiple comparisons. For each defect, a minimum of 4 samples and 6 samples were analyzed for 3T6 and EpH4, respectively.
Analysis of YAP localization was performed as in ref. 29 (link). Briefly, the nucleus of each cell was segmented using the DAPI channel, and the average intensity of YAP was measured inside the nucleus and just outside the nucleus. The nuclear YAP score was then obtained by taking the ratio of nuclear to cytoplasmic YAP mean intensity.
Statistical analysis was done in Prism using Ordinary one-way ANOVA with Tukey's test for multiple comparisons. For each defect, a minimum of 4 samples and 6 samples were analyzed for 3T6 and EpH4, respectively.