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Recombinant rankl

Manufactured by Oriental Yeast
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Recombinant RANKL is a laboratory reagent produced by Oriental Yeast. It is a protein that serves as a core component in cell signaling research and experimentation.

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Lab products found in correlation

Anti cd16 32, by BioLegend (1 mentions) Biotinylated uea 1, by Vector Laboratories (1 mentions) Anti cd45 apc cy7 clone 30 f11, by BioLegend (1 mentions) Nucleopore filter, by Cytiva (1 mentions) Whatman, by Cytiva (1 mentions)

Spelling variants of this product

RANKL (14 citations) Human recombinant soluble RANKL (4 citations)

2 protocols using recombinant rankl

1

Murine Immune Cell Profiling

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The following fluorescent anti–mouse antibodies were used for flow cytometry: anti-CD16/32, APCCy7-anti-CD45 (clone 30 F-11), APCCy7- or PECy7-anti-TER119 (clone TER-119), PE- or PECy7-anti-EpCAM (CD326, clone G8.8), FITC- or PECy7-anti-MHCII (I-A/I-E, clone M5/114.15.2), APC-anti-Ly51 (clone 6C3; BioLegend), PE-anti-CD80 (clone 16-10A1), PE-anti-CD24 (clone M1/69; eBioscience), and biotinylated UEA-1 (Vector Laboratories) antibodies. An agonistic anti-LtbR antibody was purchased from Alexis Biochemicals. Recombinant RANKL was a generous gift from Oriental Yeast Co. Anti-RANKL antibodies were prepared from a subclone of hybridoma obtained by fusing mouse myeloma cells with B cells.
Akiyama N., Takizawa N., Miyauchi M., Yanai H., Tateishi R., Shinzawa M., Yoshinaga R., Kurihara M., Demizu Y., Yasuda H., Yagi S., Wu G., Matsumoto M., Sakamoto R., Yoshida N., Penninger J.M., Kobayashi Y., Inoue J.I, & Akiyama T. (2016). Identification of embryonic precursor cells that differentiate into thymic epithelial cells expressing autoimmune regulator. The Journal of Experimental Medicine, 213(8), 1441-1458.
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2

Fetal Thymic Stroma Manipulation

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Thymic lobes were isolated from E15.5 mice and cultured for 4 d on Nucleopore filters (Whatman) placed on R10 medium containing 1.35 mM 2′-deoxyguanosine (2DG; Sigma-Aldrich). Fetal thymic stroma (2DG-FTOC) was subsequently cultured in R10 with recombinant RANKL (1 µg/ml; Oriental Yeast Co.), agonistic anti-LtbR monoclonal antibodies (1 µg/ml), or MV1 (5 µM). 5 d after ligation, 2DG-FTOC was used for the analysis.
Akiyama N., Takizawa N., Miyauchi M., Yanai H., Tateishi R., Shinzawa M., Yoshinaga R., Kurihara M., Demizu Y., Yasuda H., Yagi S., Wu G., Matsumoto M., Sakamoto R., Yoshida N., Penninger J.M., Kobayashi Y., Inoue J.I, & Akiyama T. (2016). Identification of embryonic precursor cells that differentiate into thymic epithelial cells expressing autoimmune regulator. The Journal of Experimental Medicine, 213(8), 1441-1458.
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