Rorγt clone b2d

Manufactured by Thermo Fisher Scientific

Sourced in Belgium

RORγT (clone B2D) is a monoclonal antibody that specifically binds to the retinoic acid-related orphan receptor gamma T (RORγT) protein. RORγT is a transcription factor that plays a key role in the differentiation and function of T helper 17 (Th17) cells. The antibody can be used in various applications such as flow cytometry, immunohistochemistry, and Western blotting to detect and analyze RORγT expression.

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Lab products found in correlation

Diva software, by BD (2 mentions) Cd94 clone 20d5, by BioLegend (2 mentions) Fixable viability dye fluor 780, by Thermo Fisher Scientific (2 mentions) Cd90.2 clone 53 2 1, by BD (2 mentions) Lsrfortessa sorp flow cytometer, by BD (1 mentions) Cd16 cd32 clone 2.4g2, by BD (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Cd16 cd32 fc block clone 2.4g2, by BD (1 mentions) Klrg1 clone 2f1, by BD (1 mentions)

Spelling variants of this product

RORγt (28 citations) RORγt (B2D, (7 citations)

2 protocols using rorγt clone b2d

Multicolor Flow Cytometry for Cellular Phenotyping

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Four million cells were stained with Fixable Viability Dye FluorTM 780 (eBioscience, Invitrogen, Merelbeke, Belgium) to perform a viability staining and pre-incubated with CD16/CD32 (clone 2.4G2, BD Biosciences, Erembodegem, Belgium) to block non-antigen-specific binding of immunoglobulins. Combinations of anti-mouse fluorochrome-conjugated mAbs against CD45 (clone 30-F11), CD127 (clone SB/199), CD90.2 (clone 53-2.1), KLRG-1 (clone 2F1), CD11b (clone M1/70), CD19 (clone 1D3), CD3e (clone G4.18), CD45RB (clone 16A), CD49b (Clone AK-7), CD5 (clone 53-7.3), TCRγδ (clone GL3), Ter-119 (clone TER-119) (all from BD Biosciences, Erembodegem, Belgium), RORγT (clone B2D)(ThermoFisher Scientific, Massachusetts, US), NKp46 (clone 29A1.4), Ly6G (Clone 1A8) and CD94 (clone 20d5)(all from Biolegend, San Diego, California) were used to label the cells. Sample acquisition was performed on a LSR Fortessa SORP flow cytometer running DIVA software (BD Biosciences, Erembodegem, Belgium). Data analysis was performed by using FlowJo software. Gating strategy available in Appendix A Fig. S4. Flow cytometry configuration are available in Appendix B.

Decaesteker T., Vanhoffelen E., Trekels K., Jonckheere A.C., Cremer J., Vanstapel A., Dilissen E., Bullens D., Dupont L.J, & Vanoirbeek J.A. (2021). Differential effects of intense exercise and pollution on the airways in a murine model. Particle and Fibre Toxicology, 18, 12.

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Multiparametric Flow Cytometry Protocol

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Four million cells were stained with Fixable Viability Dye Fluor TM 780 (eBioscience, Invitrogen, Merelbeke, Belgium) to perform a live/dead staining and preincubated with CD16/CD32 (FC block, clone 2.4G2, BD Biosciences, Erembodegem, Belgium). Combinations of anti-mouse fluorochrome-conjugated mAbs against CD45 (clone 30-F11), CD127 (clone SB/199), CD90.2 (clone 53-2.1), KLRG-1 (clone 2F1), CD11b (clone M1/70), CD19 (clone 1D3), CD3e (clone G4.18), CD45RB (clone 16A), CD49b (Clone AK-7), CD5 (clone 53-7.3), TCRγδ (clone GL3), Ter-119 (clone TER-119) (all from BD Biosciences, Erembodegem, Belgium), RORγT (clone B2D) (ThermoFisher Scientific, Massachusetts, US), NKp46 (clone 29A1.4), Ly6G (Clone 1A8) and CD94 (clone 20d5) (all from Biolegend, San Diego, California) were used to label the cells (gating strategy see Fig. S6). Data acquisition was performed on a LSR Fortessa flow cytometer running DIVA software (BD Biosciences, Erembodegem, Belgium). Data analysis was performed by using FlowJo software.

Decaesteker T., Jonckheere A.C., Vanhoffelen E., Schauvaerts J., Verhalle T., Cremer J., Dilissen E., Rodewald H.R., Dupont L., Bullens D.M.A, & Vanoirbeek J.A.J. (2022). Chlorine exposure and intensive exercise induces airway hyperreactivity in a 3-week murine exercise model. The Science of the total environment, 843.

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