Scrapings of the intestinal mucosa were lysed by sonication (Labsonic B. Braun, Melsungen, Germany) twice for 10 s on ice. After centrifugation (3 min at 18,000 x g), protein concentrations were determined in the supernatant according to the method of Lowry using the DC™ Protein Assay Kit (Bio-Rad Laboratories, Hercules, CA). To prove efficient intestine-specific overexpression, 80 μg of protein were separated by SDS-PAGE and transferred onto a nitrocellulose membrane, followed by incubation with an anti-FLAG antibody (#F3165; Sigma-Aldrich, ST. Louis, MO; 1:2000) or an anti-ATGL antibody (#2138; Cell Signaling Technology; Danvers, MA; 1:200). Monoclonal anti-mouse β-actin (#A5316; Sigma-Aldrich) was used as loading control. Secondary anti-mouse (FLAG; 1:500; Dako, Glostrup, Denmark) or anti-rabbit (ATGL; 1:2500; Thermo Fisher, Waltham, MA) antibodies conjugated with HRP were visualized using the Clarity™ Western ECL Substrate Kit (Bio-Rad Laboratories) on a ChemiDoc™ MP imaging system (Bio-Rad Laboratories).
Anti atgl antibody
Manufactured by Cell Signaling Technology
Sourced in Macao
The Anti-ATGL antibody is a tool for detecting and analyzing the expression of the Adipose Triglyceride Lipase (ATGL) protein. ATGL is an enzyme that plays a key role in the hydrolysis of triglycerides, the primary storage form of fatty acids in cells. This antibody can be used in various analytical techniques, such as Western blotting, to measure the levels of ATGL in biological samples.
Automatically generated - may contain errors
2 protocols using anti atgl antibody
1
Intestinal Mucosa Protein Expression
2
Intestinal Mucosa Protein Expression
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