Vector s2000

Manufactured by Vector Laboratories

Sourced in United States

The Vector S2000 is a laboratory instrument designed for general application. It features a compact design and provides basic functionality for common laboratory tasks.

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Lab products found in correlation

Protease p 8038, by Merck Group (1 mentions) M0755, by Agilent Technologies (1 mentions) Cd11c n418, by BD (1 mentions) Zytomed zytochem plus ap polymer kitpolap, by Zytomed Systems (1 mentions) Hpa012657, by Merck Group (1 mentions)

3 protocols using vector s2000

Immunohistochemical Staining Protocol for IgG and CD20

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PAS staining and electron microscopy were performed following standard protocols. For immunohistochemical staining for IgG and CD20, 1–2 µm thin slices from formalin-fixed, paraffin-embedded renal biopsies were deparaffinized and pretreated for 15 min at pH6 and 117°C in the autoclave (for CD20) or with proteinase (protease P-8038, Sigma-Aldrich, St. Louis, MO, USA) at 40°C for 15 min (for IgG) followed by incubation with normal serum (Vector S2000, CA, USA) for 10 min. Primary antibodies for IgG (1:7,500) (mouse monoclonal antibody, 209-005-088, Dianova, Hamburg, Germany) and CD20 (1:2,000) (DAKO M0755, CA, USA) were added for 30 min at 40°C. Bound antibodies were then visualized manually using a standard APAAP protocol.

Webendörfer M., Reinhard L., Stahl R.A., Wiech T., Mittrücker H.W., Harendza S, & Hoxha E. (2021). Rituximab Induces Complete Remission of Proteinuria in a Patient With Minimal Change Disease and No Detectable B Cells. Frontiers in Immunology, 11, 586012.

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Multicolor Immunofluorescence Staining of Spleen and Aortic Arch

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Spleen sections (10 μm in thickness) were fixed in ice-cold acetone and blocked with Fc-Block (CD16/CD32) in PBS 0.1% BSA prior to incubation with Alexa Fluor 488-conjugated antibody to mouse GL7 (GL7, Biolegend), APC-conjugated IgD (11-26c.2a, eBioscience) and counterstained with DAPI (Sigma D8417). For detection of immune complex deposition in the kidneys, kidneys were mounted in OCT and 6 µm thick frozen sections were blocked with normal horse serum (Vector S-2000) then stained against IgM and IgG2c whilst nuclei were DAPI stained
Aortic arches were harvested, removed surrounding fat and adventitia and fixed in 4% PFA. After they were cut open longitudinally the arch containing lesser and greater curvature was pinned on a parafilm bed. Spleen sections (10 µm in thickness) were fixed in 4% PFA. Permeabilisation was performed with 0.2% Triton X-100 in PBS. Blocking was carried out with normal horse serum followed by primary antibody (CD11c N418, biotin, Pharmingen), and secondary antibody (Streptavidin Dylight 488 conjugated (Vector SA-5488)) staining. Subsequently tissue was stained with Nile red (Sigma N3013)and finally DAPI. Confocal images were taken using a Leica TCS inverted microscope.

Centa M., Prokopec K.E., Garimella M.G., Habir K., Hofste L., Stark J.M., Dahdah A., Tibbit C.A., Polyzos K.A., Gisterå A., Johansson D.K., Maeda N.N., Hansson G.K., Ketelhuth D.F., Coquet J.M., Binder C.J., Karlsson M.C, & Malin S. (2018). Acute loss of Apolipoprotein E triggers an autoimmune response that accelerates atherosclerosis. Arteriosclerosis, thrombosis, and vascular biology, 38(8), e145-e158.

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Immunohistochemical Analysis of PLA2R1 Protein

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For PLA₂R1 immunohistochemical analyses slides were deparaffinized, pre-treated in citrate buffer (pH 6.2) for 15 min at 120°C and cooled down in iced water (10 min). After rinsing in 99% ethanol, slides were incubated for 10 min with normal serum (Vector S2000; VectorLaboratories, Burlingame, CA) followed by PLA₂R1-antibodies (polyclonal antibody from rabbit, 1:3000, HPA 012657, Sigma-Aldrich, St. Louis, MO) overnight at 4°C. The slides were then washed in PBS, incubated with polymer 1 (Zytomed Zytochem-Plus AP Polymer-KitPOLAP), rinsed in PBS and incubated with polymer 2 (Zytomed Zytochem-Plus AP Polymer-Kit POLAP). After washing in PBS, slides were stained in new fuchsin naphthol As-Bi phosphate substrate mixture (30 min) followed by 1 min of nuclear staining in hemalaun (Mayer).

Mahmud M., Pinnschmidt H.O., Reinhard L., Harendza S., Wiech T., Stahl R.A, & Hoxha E. (2019). Role of phospholipase A2 receptor 1 antibody level at diagnosis for long-term renal outcome in membranous nephropathy. PLoS ONE, 14(9), e0221293.

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