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Quick load purple low molecular weight dna ladder

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The Quick-Load Purple Low Molecular Weight DNA Ladder is a pre-stained DNA marker designed for determining the molecular weight of DNA fragments in agarose gel electrophoresis. It contains a range of DNA fragments from 100 to 1,500 base pairs, with the 500 base pair fragment being more intense for easy visualization.

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Lab products found in correlation

1 kb plus dna ladder, by Thermo Fisher Scientific (2 mentions) E gel apparatus, by Thermo Fisher Scientific (2 mentions) Tbe urea page gel, by Thermo Fisher Scientific (2 mentions) E gel ex agarose gel, by Thermo Fisher Scientific (2 mentions) Nucleospin gel, by Macherey-Nagel (1 mentions) Pcr clean up kit, by Macherey-Nagel (1 mentions)

4 protocols using quick load purple low molecular weight dna ladder

1

Evaluating Concatemer Lengths by Gel Electrophoresis

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After extension, for internal quality control, the lengths of the concatemers were evaluated by diluting 1 μl of in vitro reaction with 19 μl water. For quality control, samples were then run on 1–2% E-Gel EX agarose gels (Thermo Fisher #G402001) for 10 min on the E-gel apparatus (Invitrogen, iBase) alongside a 1 kb Plus DNA Ladder (Invitrogen) and imaged with the SybrGold channel on a Typhoon FLA 9000 scanner.
For the comparison gel in Supplementary Fig. 6, unpurified concatemers were run using 6% TBE-UREA PAGE gels (Thermo Fisher) at 55°C. The gel was pre-run for 1 h before loading the samples. 160 ng Quick-Load Purple Low Molecular Weight DNA Ladder (NEB #N0557S) was loaded as size reference. The reaction products were diluted 1:7 with 2× Urea-Loading Dye, and denatured at 95°C for 5 min. 9 μl from each sample was loaded on the gel. Both samples and the ladder were denatured. Samples were run for 20 min at 75 V, and at 130 V for 1 h. Gels were stained with 1:10,000 SybrGold in 0.5× Tris-Borate-EDTA (TBE) for 30 min and scanned on a Typhoon FLA 9000 scanner.
Saka S.K., Wang Y., Kishi J.Y., Zhu A., Zeng Y., Xie W., Kirli K., Yapp C., Cicconet M., Beliveau B.J., Lapan S.W., Yin S., Lin M., Boyden E.S., Kaeser P.S., Pihan G., Church G.M, & Yin P. (2019). Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues. Nature biotechnology, 37(9), 1080-1090.
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2

Large-Scale PCR Amplification and Purification

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The optimal reaction conditions were applied to perform large-scale PCR amplification with 40 PCR tubes, each containing 50 µL (for a final volume of 2 mL). PCR products were purified using a NucleoSpin Gel and a PCR Clean Up Kit (Macherey-Nagel, Düren, Germany), according to the manufacturer’s instructions, with the following modifications: elution was performed twice after 5 min of incubation with 15 µL of elution buffer, previously heated to 70 °C. Ten µL of PCR products were diluted to 1/5th and deposited on a 4% (w/v) agarose gel. The dsDNA concentration was determined after gel electrophoresis with a quantitative size marker (Quick-Load Purple Low Molecular Weight DNA Ladder, NEB) and electrophoretic analysis of the band intensities using the ImageJ program.
Le Dortz L.L., Rouxel C., Leroy Q., Brosseau N., Boulouis H.J., Haddad N., Lagrée A.C, & Deshuillers P.L. (2022). Optimized Lambda Exonuclease Digestion or Purification Using Streptavidin-Coated Beads: Which One Is Best for Successful DNA Aptamer Selection?. Methods and Protocols, 5(6), 89.
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3

Evaluating Concatemer Lengths by Gel Electrophoresis

Check if the same lab product or an alternative is used in the 5 most similar protocols
After extension, for internal quality control, the lengths of the concatemers were evaluated by diluting 1 μl of in vitro reaction with 19 μl water. For quality control, samples were then run on 1–2% E-Gel EX agarose gels (Thermo Fisher #G402001) for 10 min on the E-gel apparatus (Invitrogen, iBase) alongside a 1 kb Plus DNA Ladder (Invitrogen) and imaged with the SybrGold channel on a Typhoon FLA 9000 scanner.
For the comparison gel in Supplementary Fig. 6, unpurified concatemers were run using 6% TBE-UREA PAGE gels (Thermo Fisher) at 55°C. The gel was pre-run for 1 h before loading the samples. 160 ng Quick-Load Purple Low Molecular Weight DNA Ladder (NEB #N0557S) was loaded as size reference. The reaction products were diluted 1:7 with 2× Urea-Loading Dye, and denatured at 95°C for 5 min. 9 μl from each sample was loaded on the gel. Both samples and the ladder were denatured. Samples were run for 20 min at 75 V, and at 130 V for 1 h. Gels were stained with 1:10,000 SybrGold in 0.5× Tris-Borate-EDTA (TBE) for 30 min and scanned on a Typhoon FLA 9000 scanner.
Saka S.K., Wang Y., Kishi J.Y., Zhu A., Zeng Y., Xie W., Kirli K., Yapp C., Cicconet M., Beliveau B.J., Lapan S.W., Yin S., Lin M., Boyden E.S., Kaeser P.S., Pihan G., Church G.M, & Yin P. (2019). Immuno-SABER enables highly multiplexed and amplified protein imaging in tissues. Nature biotechnology, 37(9), 1080-1090.
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4

RPA Assay for Molecular Detection

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The RPA assay was performed referring to the TwistDx's recommended protocols (https://www.twistdx.co.uk/en/products/product/twistamp-basic). The reaction mixture containing 29.5 µL of a rehydration buffer, 2.4 µL of 10 µM forward and reverse primer, 12.2 µL of sterile water and 1 µL of the template was transferred to a lyophilized pellet tube. Then, 2.5 µL of magnesium acetate was added to start the reaction. Sterile water was used as a negative control sample. The tubes were mixed thoroughly and then centrifuged briefly. Subsequently, the tubes were incubated at 39°C for 25 min in BioSan Dry block thermostat Bio TDB-100. A mixing step after 4 min of incubation was carried out for better sensitivity of the assay. Finally, 50 µL of PCI was added to the tubes and vortexed lightly. The tubes were centrifuged at 14000 g for 10 min to remove the undesirable components affecting the read-out of the results. The RPA products were analyzed by a 1.5% agarose gel electrophoresis and visualized under the UV light using G: BOX Mini 6/9 (Syngene, Cambridge, UK). HyperLadder TM 100 bp (Meridian Bioscience, Bioline, London, UK) and Quick-Load Purple Low Molecular Weight DNA Ladder (New England Biolabs, MA, USA) were used as the DNA ladder.
Tran H.T., Tran D.H., Pham T.N.M., & Phùng H.T.T. (2022). DIRECT RECOMBINASE POLYMERASE AMPLIFICATION ASSAY FOR ACCURATE AND RAPID DETECTION OF LISTERIA MONOCYTOGENES IN FOOD. Journal of Microbiology Biotechnology and Food Sciences, 11(5), e4749-e4749.
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