OSI, rociletinib, afatinib, erlotinib, gefitinib, pemetrexed, navitoclax, and Z-VAD-FMK were purchased from Selleck Chemicals (Houston, TX, USA). Paclitaxel, doxorubicin, fluorouracil, paraformaldehyde (PFA), MTT, and dimethyl sulfoxide (DMSO) were obtained from Sigma (St. Louis, MO, USA). Crystal violet was purchased from Beyotime Biotechnology Corporation-Shanghai (Shanghai, China). RPMI 1640 medium, fetal bovine serum (FBS), penicillin, streptomycinwere, and phosphate-buffered saline (PBS) were purchased from Gibco Life Technologies (Grand Island, NY, USA). The primary antibodies, i.e. PCNA, MMP2, TIMP2, p-FAK (Tyr397), FAK, p-Src (Tyr416), Src, p-Paxillin (Tyr118), Paxillin, P-gp, p-EGFR (Tyr1068), EGFR, p-ERK (Thr202/Tyr204), ERK, p-AKT (Ser473), AKT, c-PARP, c-caspase 3, RB, GAPDH, and the responsive secondary antibodies were obtained from Cell Signaling Technology (Beverly, MA, USA).
Streptomycinwere
Manufactured by Thermo Fisher Scientific
Sourced in United States
Streptomycinwere is a laboratory equipment used for the detection and quantification of the antibiotic streptomycin. It is a device designed to provide accurate and reliable measurements of streptomycin levels in various samples, such as pharmaceutical products, biological samples, or environmental matrices.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (3 mentions)
Penicillin, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (3 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (3 mentions)
Gapdh, by Cell Signaling Technology (2 mentions)
Dulbecco s modified eagle s medium dmem medium, by Thermo Fisher Scientific (2 mentions)
Proliferating cell nuclear antigen (pcna), by Cell Signaling Technology (1 mentions)
Rociletinib, by Selleck Chemicals (1 mentions)
C parp, by Cell Signaling Technology (1 mentions)
Gefitinib, by Selleck Chemicals (1 mentions)
Pemetrexed, by Selleck Chemicals (1 mentions)
Crystal violet, by Beyotime (1 mentions)
Doxorubicin, by Merck Group (1 mentions)
Paclitaxel, by Merck Group (1 mentions)
Paxillin, by Cell Signaling Technology (1 mentions)
Fluorouracil, by Merck Group (1 mentions)
Afatinib, by Selleck Chemicals (1 mentions)
Erlotinib, by Selleck Chemicals (1 mentions)
P paxillin tyr118, by Cell Signaling Technology (1 mentions)
3 protocols using streptomycinwere
1
Anticancer Drug Screening Assay
2
Evaluation of CHE Cytotoxicity and Mechanisms
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CHE, with the purity of 99.34% that determined by using high-performance liquid chromatography assay, was purchased from the National Institutes for Food and Drug Control (Beijing, China) and dissolved in dimethyl sulfoxide (DMSO) at a concentration of 40 mM, stored at −20 °C. 3-(4,5-dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium bromide (MTT), chloroquine (CQ), puromycin, paraformaldehyde (PFA), and DMSO were obtained from the Sigma (St. Louis, MO, USA). Dulbecco's modified Eagle's medium (DMEM) medium, RPMI 1640 medium, fetal bovine serum (FBS), penicillin, streptomycinwere, phosphate-buffered saline (PBS), and hanks' balanced salt solution (HBSS) were obtained from the Gibco Life Technologies (Grand Island, NY, USA). 2′,7′-dichlorofluorescin-diacetate (DCFH2-DA) and crystal violet staining solution were purchased from the Beyotime Biotechnology Corporation (Shanghai, China). The primary antibodies, i.e. poly (ADP-ribose) polymerase (PARP) (#9532), cleaved caspase 3 (#9664), LC3 (#12741), beclin 1 (#3495), GAPDH (#2118), and anti-rabbit IgG, HRP-linked (#7074) were obtained from Cell Signaling Technology (Beverly, MA, USA).
3
Comprehensive Molecular Mechanisms Study
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LCA was purchased from National Institutes for Food and Drug Control (Beijing, China) and dissolved in dimethyl sulfoxide (DMSO) at a concentration of 40 mM, stored at −20 °C. MTT, CQ, G418, and DMSO were obtained from Sigma (St. Louis, MO, USA). 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Beyotime Biotechnology Corporation–Shanghai (Shanghai, China). Dulbecco’s modified Eagle’s medium (DMEM) medium, RPMI 1640 medium, fetal bovine serum (FBS), penicillin, streptomycinwere, phosphate-buffered saline (PBS), and PI obtained from Gibco Life Technologies (Grand Island, NY, USA). GFP-LC3 (supplied by Toren Finkel, Addgene plasmid #24920)51 (link) were obtained from Addgene. The primary antibodies, i.e. PARP, cleaved-caspase 7, cleaved-caspase 3, LC3, ATG7, ATG5, CHOP, BIP, GAPDH, and the responsive secondary antibodies were obtained from Cell Signaling Technology Inc. (Beverly, MA, USA).
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