Vdac ab14734

Manufactured by Abcam

VDAC (ab14734) is a laboratory reagent that functions as a voltage-dependent anion channel. It is a protein that forms pores in the outer mitochondrial membrane, allowing the passage of small molecules and ions between the cytosol and mitochondria. The product is intended for research use only and its specific applications should be determined by the user.

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Lab products found in correlation

Irdye 680lt goat anti mouse igg, by LI COR (1 mentions) Gapdh 2118, by Cell Signaling Technology (1 mentions) Odyssey infrared imaging system, by LI COR (1 mentions) Irdye 800cw goat anti rabbit immunoglobulin g, by LI COR (1 mentions) Sdha ab14715, by Abcam (1 mentions) Letm1 16024 1 ap, by Proteintech (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Anti pan actin antibody, by Merck Group (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Hsp60, by Santa Cruz Biotechnology (1 mentions) Mcl 1 sc 819, by Santa Cruz Biotechnology (1 mentions) Vcp ab109240, by Abcam (1 mentions)

Spelling variants of this product

Ab14734 (79 citations)

2 protocols using vdac ab14734

Immunoblotting Analysis of Metabolic Proteins

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Specific proteins were detected using the following rabbit monoclonal antibodies: phospho-SAPK/JNK (Thr¹⁸³/Tyr¹⁸⁵, 4668), SAPK/JNK (9252), phospho-Akt (Ser⁴⁷³, 4060), Akt (9272), S6 ribosomal protein (2217S), phospho–S6 ribosomal protein (4856S), glyceraldehyde phosphate dehydrogenase (GAPDH) (2118), LC3A/B (12741) MCU (14997) (Cell Signaling Technologies), and MRPP3 (HPA020459) (Sigma-Prestige) diluted 1:1000; rabbit polyclonal antibodies: GLUT2 (54460) (Abcam) diluted 1:1000, GLUT4 (PA-1-1065) (Thermo Fisher Scientific), CPTII (OAAN00972) (Aviva System Biology) diluted 1:500, Letm1 (16024-1-AP) (Proteintech) diluted 1:1000, MRPP2 (HPA001432) (Sigma-Prestige) diluted 1:1000, LRPPRC (sc66844) (Santa Cruz Biotechnology) diluted 1:500, TFAM (ab131607) (Abcam) diluted 1:1000; mouse monoclonal antibodies: total OXPHOS Cocktail Antibody (ab110412), VDAC (ab14734), and SDHA (ab14715) (Abcam), diluted 1:1000. IRDye 800CW goat anti-rabbit immunoglobulin G (IgG) or IRDye 680LT goat anti-mouse IgG (LI-COR Biosciences) secondary antibodies were used, and the immunoblots were visualized using an Odyssey infrared imaging system (LI-COR Biosciences). Relative amount of protein was determined using the Image Studio Lite Software.

Rossetti G., Ermer J.A., Stentenbach M., Siira S.J., Richman T.R., Milenkovic D., Perks K.L., Hughes L.A., Jamieson E., Xiafukaiti G., Ward N.C., Takahashi S., Gray N., Viola H.M., Hool L.C., Rackham O, & Filipovska A. (2021). A common genetic variant of a mitochondrial RNA processing enzyme predisposes to insulin resistance. Science Advances, 7(39), eabi7514.

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Protein Isolation and Identification Techniques

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Cycloheximide (CHX) and the protease inhibitor cocktail were purchased from Sigma–Aldrich. Proteasome inhibitor MG132 and VCP inhibitor DBeQ were from Tocris Bioscience. Antibodies against c-Myc (sc-40, 1:1000), GFP (sc-9996, 1:1000), HA (haemagglutinin, sc-7392, 1:1000), ubiquitin (sc-271289, 1:1000), HSP60 (sc-13115, 1:1000), MCL1 (sc-819, 1:1000), NPL4 (sc-134746, 1:1000), and p47 (sc-376614, 1:1000) were from Santa Cruz Biotechnology. VDAC (ab14734, 1:2000), VCP (ab109240, 1: 10,000), and UBXD1 (ab103651, 1:1000) antibodies were from Abcam. Anti-pan-actin antibody (A1978, 1: 10,000) was from Sigma–Aldrich. Anti-MFN1 (H00055669-M04, 1:1000) and MFN2 (H00009927-M01, 1:1000) antibodies were from Abnova and anti-UFD1L (NBP1-03339, 1:1000) was from Novus. Anti-mouse and Anti-rabbit IgG antibodies were from Thermo Scientific.

Guo X, & Qi X. (2016). VCP cooperates with UBXD1 to degrade mitochondrial outer membrane protein MCL1 in model of Huntington’s disease. Biochimica et biophysica acta, 1863(2), 552-559.

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