Masson trichrome with aniline blue

Manufactured by Bio-Optica

Sourced in Italy

Masson trichrome with aniline blue is a histological staining technique used to differentiate collagen, muscle, and nuclei in tissue samples. It provides a clear visual distinction between these cellular components.

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Lab products found in correlation

Paraformaldehyde (pfa), by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Mc120 hd camera, by Leica (1 mentions) Dmrb light microscope, by Leica (1 mentions) Paraffin wax, by Bio-Optica (1 mentions) Masson s trichrome, by Bio-Optica (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions) Peroxidase dab dako real envision detection system, by Agilent Technologies (1 mentions) Phospho p38, by Cell Signaling Technology (1 mentions) Caveolin 1, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

Masson trichrome (19 citations) Aniline blue (2 citations)

3 protocols using masson trichrome with aniline blue

Histological Analysis of N. guentheri Brain

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In this investigation, we used paraffin-embedded tissue of 1-year-old N. guentheri from earlier studies [32 (link)]. One-year-old, male, and female adult N. guentheri specimens from ornamental aquariums were employed. They were found dead of unexplained causes. The heads were rapidly removed and stored in 4% paraformaldehyde (Sigma-Aldrich, Inc., St. Louis, MO, USA, #158127) in 0.1 m (pH = 7.4) of phosphate-buffered saline (PBS, Sigma-Aldrich, Inc., St. Louis, MO, USA, # P4417) for 12–18 h, dehydrated by graded ethanol series, clarified in xylene, and used for paraffin wax (Bio-Optica S.p.a. Milano, Italy, # 08–7910) embedding. The included tissues were then cut into serial sections that were 7 μm thick and collected on gelatin-coated microscope slides [47 (link)]. Later, deparaffinized and rehydrated serial slices were washed in distilled water, processed with Masson trichrome with aniline blue (Bio-Optica S.p.a Milano, Italy, cat. #04-010802) [33 (link)]. At the end, stained sections were examined under a Leica DMRB light microscope equipped with Leica MC 120 HD camera (Leica Application Suite LAS V4.7).

Aragona M., Briglia M., Porcino C., Mhalhel K., Cometa M., Germanà P.G., Montalbano G., Levanti M., Laurà R., Abbate F., Germanà A, & Guerrera M.C. (2023). Localization of Calretinin, Parvalbumin, and S100 Protein in Nothobranchius guentheri Retina: A Suitable Model for the Retina Aging. Life, 13(10), 2050.

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Quantifying Collagen Deposition in ApoE-/- Murine Hearts

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Hearts from controls and chronically irradiated (0.3 and 6 Gy) ApoE^−/− mice were fixed in 10% buffered formalin and embedded in paraffin before being sectioned (in thickness of 7 μm) and stained with Masson’s trichrome according to a standard protocol (Masson trichrome with aniline blue, Bio-Optica). The collagen deposition was evaluated in the right ventricle (RV) using the HistoQuest 2.0.2.0249 software (TissueGnostics, Vienna, Austria) and expressed as a percentage of the total RV area.

Azimzadeh O., Merl-Pham J., Subramanian V., Oleksenko K., Krumm F., Mancuso M., Pasquali E., Tanaka IB I.I.I., Tanaka S., Atkinson M.J., Tapio S, & Moertl S. (2023). Late Effects of Chronic Low Dose Rate Total Body Irradiation on the Heart Proteome of ApoE−/− Mice Resemble Premature Cardiac Ageing. Cancers, 15(13), 3417.

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Immunohistochemical Analysis of Septic Lung

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Paraffin sections (5 μm thick) were deparaffinized in xylol, rehydrated in a graded ethanol series, and subjected to microwave antigen retrieval (3 × 10 min, 800 W, in 0.1 M citrate acid buffer solution, pH 6). Endogenous peroxidase activity was blocked using 0.1% hydrogen peroxide. Sections were incubated overnight at 4°C with the following primary antibodies: Caveolin 1 (Cell Signalling 3267, 1:200), phospho Akt (Cell Signaling Technology #4060 dilution 1:50), phospho P38 (Cell Signaling Technology #4511, dilution 1:50), and S100 (DAKO Z0311, dilution 1:100). Immunohistochemical staining was performed using the Dako REAL EnVision Detection System Peroxidase/DAB+ (DAKO, Denmark Cat# K5007) as instructed by manufacturer. Finally, sections were counterstained with Mayer hematoxylin, dehydrated, and mounted.
To assess the degree of fibrosis in septic lung, paraffin sections were stained with Masson's trichrome according to the protocol suggested by the manufacturer (Masson trichrome with aniline blue; Bio-Optica, Italy Cat #04-010802). Cytoplasm was stained red, collagen blue, and cell nuclei black.

Kataki A., Karagiannidis I., Memos N., Koniaris E., Antonakis P., Papalois A., Zografos G.C, & Konstadoulakis M.M. (2018). Host's Endogenous Caveolin-1 Expression is Downregulated in the Lung During Sepsis to Promote Cytoprotection. Shock (Augusta, Ga.), 50(2).

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