LacZ MF in isolated sperm DNA was determined by a positive selection assay as described in [O'Brien et al.,
Transpack packaging extract kits
Manufactured by Agilent Technologies
Sourced in Canada
The Transpack Packaging Extract kits are designed for the extraction and purification of nucleic acids from various biological samples. The kits utilize a silica-based membrane technology to capture and purify DNA or RNA, making them suitable for a range of downstream applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using transpack packaging extract kits
1
Positive Selection Assay for LacZ Mutations
Check if the same lab product or an alternative is used in the 5 most similar protocols
LacZ MF in isolated sperm DNA was determined by a positive selection assay as described in [O'Brien et al.,2014 ]. Briefly, λgt10 phage vectors were recovered from genomic DNA (∼1–4 µg) using Transpack Packaging Extract kits (Agilent Technologies, Mississauga, ON, Canada) according to the manufacturer's instructions. The packaged phages were used to infect a lacZ−/galE− E. coli host. The infected bacteria were plated on agar plates containing 0.3% phenyl‐β‐d ‐galactopyranoside (P‐Gal) to detect mutants, or agar without P‐Gal to determine the total number of plaque‐forming units (pfu). A minimum of 130,000 total pfu were scored for each animal (average = 190,000 pfu. The MF was calculated by dividing the number of mutant plaques by the total pfu count. The induced response was compared with the control group with the glm function in R, using the quasibinomial error distribution to account for overdispersion in the data. The resulting P values were adjusted for multiple comparisons using a Bonferroni correction. The no observable genotoxic effect level (NOGEL) was identified as the highest dose where MF was not significantly greater than control (P > 0.05).
+ Expand
LacZ MF in isolated sperm DNA was determined by a positive selection assay as described in [O'Brien et al.,
2
Measuring Transgene Mutation Frequency
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Genomic DNA was isolated from spermatozoa from the cauda epididymis by digestion with proteinase K and β-mercaptoethanol, followed by phenol/chloroform extraction, and ethanol precipitation as described previously14 . The lacZ MF was determined in genomic DNA as previously described14 . Briefly, viral transgene vectors were recovered from ~1–4 μg of DNA using Transpack Packaging Extract kits (Agilent Technologies, Mississauga, ON, Canada) according to the manufacturer’s instructions. E. Coli (lacZ−/galE−) were infected with the recovered viral vectors and grown on agar containing 0.3% phenyl-β-D-galactopyranoside (P-Gal) to detect mutants, or on agar without P-Gal to determine the total number of plaque-forming units (pfu). A minimum of 125,000 total pfu were scored for each animal. MF was calculated as the number of mutant plaques divided by the total pfu count. Statistical differences in MF between treatment groups were determined by logistic regression using the glm function in R53 with a quasibinomial error distribution to account for overdispersion of the data.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!