Bacillus subtilis wdcm 0003 vitroids

Iodine (≥99.0%), copper iodide (CuI) and Mueller Hinton Broth (MHB) were obtained from Sigma Aldrich (Gillingham, UK). 1,4,7,10-Tetraoxacyclododecan (12-crown-4) was received from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Disposable sterilized Petri dishes with Mueller Hinton II agar and McFarland standard sets were bought from Liofilchem Diagnostici (Roseto degli Abruzzi (TE), Italy). The bacterial strains E. coli WDCM 00013 Vitroids, P. aeruginosa WDCM 00026 Vitroids, K. pneumoniae WDCM00097 Vitroids, C. albicans WDCM 00054 Vitroids, and Bacillus subtilis WDCM0003 Vitroids were received from Sigma-Aldrich Chemical Co. S. pneumoniae ATCC 49619, S. aureus ATCC 25923, E. faecalis ATCC 29212, S. pyogenes ATCC 19615, P. mirabilis ATCC 29906 were purchased from Liofilchem. Gentamicin (9125, 30 µg/disc), cefotaxime (9017, 30 µg/disc), chloramphenicol (9128, 10 µg/disc), streptomycin (SD031, 10 µg/disc), and nystatin (9078, 100 IU/disc) were obtained from Liofilchem. Methanol (analytical grade) was received from EMSURE (Merck KGaA, Darmstadt, Germany). Acetonitrile (analytical grade) was purchased from MTEDIA (TEDIA Company, Fairfield, OH, USA). Sterile filter paper discs with a diameter of 6 mm were bought from Himedia (Mumbai, India). Ultrapure water was utilized and all reagents were of analytical grade and used as received.

Sabouraud Dextrose broth, Mueller–Hinton Broth (MHB), and silver nitrate were purchased from Sigma Aldrich (St. Louis, MO, USA). The microbial strains E. coli WDCM 00013 Vitroids, P. aeruginosa WDCM 00026 Vitroids, K. pneumoniae WDCM 00097 Vitroids, C. albicans WDCM 00054 Vitroids, and Bacillus subtilis WDCM 0003 Vitroids were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Liofilchem (Roseto degli Abruzzi, Teramo, Italy) delivered S. pneumoniae ATCC 49619, S. aureus ATCC 25923, E. faecalis ATCC 29212, S. pyogenes ATCC 19615, and P. mirabilis ATCC 29906. Disposable sterilized Petri dishes with Mueller–Hinton II agar, McFarland standard sets, gentamicin (9125, 30 µg/disc), and nystatin (9078, 100 IU/disc) were obtained from Liofilchem Diagnostici (Roseto degli Abruzzi, Teramo, Italy). Sterile filter paper discs with 6 mm diameter were obtained from Himedia (Jaitala Nagpur, Maharashtra, India). Curly garden cress (Lepidium sativum L., (LS)) seeds were purchased from the local market. The seeds were planted and harvested during November 2020 to April 2021. Ultrapure water was used in the extraction and green synthesis instead of distilled water. All reagents were used as delivered and were of analytical grade.

Iodine (≥ 99.0%), polyvinylpyrrolidone (PVP-K-30), ethanol (analytical grade), Sabouraud Dextrose broth, and Mueller Hinton Broth (MHB) were obtained from Sigma Aldrich (St. Louis, MO, USA). Disposable sterilized Petri dishes with Mueller Hinton II agar, McFarland standard sets, gentamicin (9125, 30 µg/disc) and nystatin (9078, 100 IU/disc) were purchased from Liofilchem Diagnostici (Roseto degli Abruzzi (TE), Italy). The bacterial strains E. coli WDCM 00013 Vitroids, P. aeruginosa WDCM 00026 Vitroids, K. pneumoniae WDCM 00097 Vitroids, C. albicans WDCM 00054 Vitroids, and Bacillus subtilis WDCM 0003 Vitroids were received from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). S. pneumoniae ATCC 49619, S. aureus ATCC 25923, E. faecalis ATCC 29212, S. pyogenes ATCC 19615 andP. mirabilis ATCC 29906 were purchased from Liofilchem (Roseto degli Abruzzi (TE), Italy). Sterile polyglycolic acid (PGA) surgical sutures (DAMACRYL, 75 cm, USP: 3-0, Metric:2, 19 mm, DC3K19) were purchased from General Medical Disposable (GMD), GMD Group A.S., Istanbul, Turkey. Sterile filter paper discs with a diameter of 6 mm were purchased from Himedia (Jaitala Nagpur, Maharashtra, India). Aloe vera leaves (Aloe barbadensis Miller, AV) were harvested from the botanical garden of Ajman University campus, Ajman, UAE. Ultrapure water was utilized. All reagents were of analytical grade and used as delivered.