From all the study participants, venous blood samples into serum clot activator tubes were collected using the standard venipuncture techniques between 8 A.M. and 11 A.M. Due to the overnight fasting, all participants were in a fasted state. The filled clot activator tube, within an hour of collection, was then centrifuged at 2000× g for 10 min and then placed into Cobas 6000’s module e601 (Roche Diagnostics, Mannheim, Germany) in order to assess the level of 25-hydroxyvitamin D.
Vitamin D (25-hydroxyvitamin D) was assessed on Cobas 6000’s module e601 (Roche Diagnostics, Mannheim, Germany) using electrochemiluminescence. Levels of vitamin D above or equal to 30 µg/L were considered optimal, levels between 21 to 29 µg/L were considered insufficient, and levels below or equal to 20 µg/L were considered to be indicative of deficiency.
All determinations conducted in this study were in accordance with the manufacturer’s instructions as well as the internal laboratory standards.
Vitamin D (25-hydroxyvitamin D) was assessed on Cobas 6000’s module e601 (Roche Diagnostics, Mannheim, Germany) using electrochemiluminescence. Levels of vitamin D above or equal to 30 µg/L were considered optimal, levels between 21 to 29 µg/L were considered insufficient, and levels below or equal to 20 µg/L were considered to be indicative of deficiency.
All determinations conducted in this study were in accordance with the manufacturer’s instructions as well as the internal laboratory standards.