Hap1 TP53 cells were cultured in IMDM media and supplemented with 10% Fetal-GRO and 5% penicillin and streptomycin (Gibco) and L-glutamine (Gibco). Flp-In T-REx 293 cells AUF1 cell line was cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco) and supplemented with 10% Heat-Inactivated Fetal Bovine Serum (Gibco), 5% minimum essential medium nonessential amino acids (100 × , Gibco), 5% penicillin and streptomycin (Gibco) and Lglutamine (Gibco).
Additionally, 5 μg ml-1 of blasticidin and 100 μg ml-1 of Zeocin was added to media for cell line maintenance. Flp-In T-REx 293 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco) and supplemented with 10% Fetalgro® Bovine Growth Serum (BGS), 5% minimum essential medium nonessential amino acids (100 × , Gibco), 5% penicillin, streptomycin (Gibco), and L-glutamine (Gibco). Additionally, 5 μg ml-1 of blasticidin and 100 μg ml-1 of Zeocin was added to the media for cell line maintenance. CHO-K cells were cultured in F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) (Gibco) supplemented with 10% Fetalgro® Bovine Growth Serum (BGS), 5% minimum essential medium nonessential amino acids (100 × , Gibco), 5% penicillin and streptomycin (Gibco), and L-glutamine (Gibco).
Additionally, 5 μg ml-1 of blasticidin and 100 μg ml-1 of Zeocin was added to media for cell line maintenance. Flp-In T-REx 293 cells were cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco) and supplemented with 10% Fetalgro® Bovine Growth Serum (BGS), 5% minimum essential medium nonessential amino acids (100 × , Gibco), 5% penicillin, streptomycin (Gibco), and L-glutamine (Gibco). Additionally, 5 μg ml-1 of blasticidin and 100 μg ml-1 of Zeocin was added to the media for cell line maintenance. CHO-K cells were cultured in F-12K Medium (Kaighn's Modification of Ham's F-12 Medium) (Gibco) supplemented with 10% Fetalgro® Bovine Growth Serum (BGS), 5% minimum essential medium nonessential amino acids (100 × , Gibco), 5% penicillin and streptomycin (Gibco), and L-glutamine (Gibco).