The hUC-MSCs were seeded onto 10 × 4 × 3 mm nHAC/PLA scaffolds in 24-well plates at a density of 1 × 107 cells/cm2 per sample and were cultured in 1 ml mentioned above medium for 7 days. The constructs were fixed with 2% paraformaldehyde and 2.5% glutaraldehyde (Merck KGaA) in 0.1 mol/l phosphate buffer for 48 h at room temperature and were then rinsed with PBS, different concentrations of ethanol, and different concentrations of hexamethyldisilazane. The construct were glued with conducing paste (catalog no. C680548, 8 mm × 20 m, Nissin EM Co., Ltd.) to appropriate mounting stabs and coated with a several nanometer-thick layer of gold and examined under a Hitachi S-520 scanning electron microscope.
S 520 scanning
Manufactured by Hitachi
The S-520 is a scanning electron microscope (SEM) manufactured by Hitachi. The core function of the S-520 is to produce high-resolution images of small-scale samples by scanning them with a focused beam of electrons. The S-520 is capable of magnifying samples up to 300,000 times, enabling detailed examination of surface topography and microstructures.
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Lab products found in correlation
2 protocols using s 520 scanning
1
Scaffold-Based hUC-MSC Cultivation and Imaging
2
Lung Microstructure Casting and SEM Analysis
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Proper small pieces of specimens were collected from different extracted lung lobes, and were then cut into SEM specimens and placed into 10 % hydrochloric acid at room temperature for 2 weeks. Later, they were washed in flowing water in a net box slowly. Then, the residues were washed out, and the casts were selected, dried and bumble-coated with gold-palladium and examined under a Hitachi S520 scanning electron microscope.
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