D7 pe

Manufactured by Avanti Polar Lipids

Sourced in United States

D7-PE is a phospholipid compound produced by Avanti Polar Lipids. It is a deuterated form of phosphatidylethanolamine, which is a common membrane lipid. The deuterium labeling provides a stable isotopic signature that can be used in various analytical applications.

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Lab products found in correlation

D7 lpc, by Avanti Polar Lipids (2 mentions) Accela 1250, by Thermo Fisher Scientific (1 mentions) Q exactive orbitrap mass spectrometer, by Thermo Fisher Scientific (1 mentions) Cholesterol d7, by Avanti Polar Lipids (1 mentions)

2 protocols using d7 pe

Lipidomic Analysis of Cell Lines

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GC grade dichloromethane (DCM), ammonium acetate, and ammonium hydroxide and 2-propanol (IPA) were supplied by Fisher Scientific (Waltham, MA, USA). LC-MS grade methanol (MeOH) and methyl tert-butyl ether (MTBE) were supplied by CNW Technologies (Stuart, FL, USA). Lipid standards including d7-PE, d7-LPC, and d7-TG were supplied by Avanti Polar Lipids (Alabaster, AL, USA).
The CAL-27 cell line was provided by Professor Youguang Lu of the Stomatology School, Fujian Medical University. The HOK cell line was provided by Ruijin Hospital, Shanghai Jiao Tong University School of Medicine.

Wang X.Y., Zhang T., Guan W.Q., Li H.Z, & Lin L. (2021). A Study of the Lipidomic Profiles of the CAL-27 and HOK Cell Lines Using EMS Spectra. Frontiers in Oncology, 11, 771337.

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Lipid Profiling Using UHPLC-MS

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Dried lipophilic cell extracts were reconstituted with 200 μL of a methanol:isopropanol:water mixture (65:30:5 by volume)(24 (link)) that contained an internal standard mixture made from the SPLASH Lipidomix standard mixture that included d7-PC(15:0/18:1), d7-PE(15:0/18:1), d7-PG(15:0/18:1), d7-PI(15:0/18:1), d7-PS(15:0/18:1), d7-LPC(18:1), d7-LPE(18:1), d7-TG(15:0/18:1/15:0), d7-DG(15:0/18:1), d7-MG(18:1), d9-SM(d18:1/18:1), d7-CE(18:1), and d7-cholesterol (Avanti Polar Lipids, Alabaster, AL, USA), Cer(d18:1/12:0) (Avanti Polar Lipids), and d27-myristic acid (Sigma-Aldrich, St. Louis, MO, USA). The reconstituted samples were then profiled using an Accela 1250 ultrahigh-performance liquid chromatography (UHPLC) system coupled to a Q-Exactive orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA). Details regarding methods and instrumental settings are provided in SI. Quality control (QC) samples were prepared by pooling an equal amount (∼20%) of all processed samples and distributing aliquots into 8 vials after complete mixing. A QC sample was injected at the beginning, end, and after every 10 samples of the run.

Zhen H., Teng Q., Mosley J.D., Collette T.W., Yue Y., Bradley P.M, & Ekman D.R. (2021). Untargeted Lipidomics for Determining Cellular and Subcellular Responses in Zebrafish (Danio rerio) Liver Cells Following Exposure to Complex Mixtures in U.S. Streams. Environmental science & technology, 55(12), 8180-8190.

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