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Sn33623

Manufactured by Merck Group
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The SN33623 is a laboratory equipment product manufactured by Merck Group. It is designed for general laboratory use. The core function of this product is to perform specific tasks within a controlled laboratory environment. Further details about the intended use or features of this product are not available.

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Lab products found in correlation

Pet28a, by Addgene (2 mentions)

2 protocols using sn33623

1

Cloning and Purification of NTR Variants

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Genes encoding NTR candidates were PCR amplified and cloned into the NdeI and SalI sites of two plasmids: pUCX (Addgene #60681), for bacterial overexpression assays, and pET28a(+) (Addgene #69864–3), for His6-tag protein purification. To assess NTR variant properties in the absence of native NTR activity, the E. coli 7NT strain was used, which bears scarless in-frame deletions of seven candidate NTR genes (nfsA, nfsB, azoR, nemA, yieF, ycaK and mdaB) and the efflux pump gene tolC56 (link). For protein purification, the E. coli strain BL21(DE3) was used. All reagents were purchased from Sigma-Aldrich, apart from SN33623, which was custom-synthesized by BOC Sciences.
Sharrock A.V., Mulligan T.S., Hall K.R., Williams E.M., White D.T., Zhang L., Emmerich K., Matthews F., Nimmagadda S., Washington S., Le K.D., Meir-Levi D., Cox O.L., Saxena M.T., Calof A.L., Lopez-Burks M.E., Lander A.D., Ding D., Ji H., Ackerley D.F, & Mumm J.S. (2022). NTR 2.0: a rationally-engineered prodrug converting enzyme with substantially enhanced efficacy for targeted cell ablation. Nature methods, 19(2), 205-215.
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2

Cloning and Purification of NTR Variants

Check if the same lab product or an alternative is used in the 5 most similar protocols
Genes encoding NTR candidates were PCR amplified and cloned into the NdeI and SalI sites of two plasmids: pUCX (Addgene #60681), for bacterial overexpression assays, and pET28a(+) (Addgene #69864–3), for His6-tag protein purification. To assess NTR variant properties in the absence of native NTR activity, the E. coli 7NT strain was used, which bears scarless in-frame deletions of seven candidate NTR genes (nfsA, nfsB, azoR, nemA, yieF, ycaK and mdaB) and the efflux pump gene tolC56 (link). For protein purification, the E. coli strain BL21(DE3) was used. All reagents were purchased from Sigma-Aldrich, apart from SN33623, which was custom-synthesized by BOC Sciences.
Sharrock A.V., Mulligan T.S., Hall K.R., Williams E.M., White D.T., Zhang L., Emmerich K., Matthews F., Nimmagadda S., Washington S., Le K.D., Meir-Levi D., Cox O.L., Saxena M.T., Calof A.L., Lopez-Burks M.E., Lander A.D., Ding D., Ji H., Ackerley D.F, & Mumm J.S. (2022). NTR 2.0: a rationally-engineered prodrug converting enzyme with substantially enhanced efficacy for targeted cell ablation. Nature methods, 19(2), 205-215.
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