H37ra atcc 25177

Mtb H37Rv (ATCC 27294) and H37Ra (ATCC 25177) were purchased from American Type Culture Collection (ATCC, Manassas, VA), and Mtb HN878 was obtained from the strain collections of the International Tuberculosis Research Center (ITRC, Changwon, Gyeongsangnam-do, South Korea). M. bovis BCG (Pasteur strain 1173P2) was kindly provided by Dr. Brosch at the Pasteur Institute (Paris, France). All mycobacteria used in this study were prepared as described previously [25 (link)].

Mtb H37Rv (ATCC 27294) and H37Ra (ATCC 25177) were purchased from American Type Culture Collection (ATCC, Manassas, VA), and the Mtb K strain was obtained from the strain collections at the Korean Institute of Tuberculosis (Korean National Tuberculosis Association, Seoul, Republic of Korea). All strains were cultured in Middlebrook 7H9 broth (Difco Laboratories, Detroit, MI) supplemented with 0.02% glycerol and 10% (vol/vol) oleic acid-albumin-dextrose-catalase (OADC, Becton Dickinson, Sparks, MD) for 28 days at 37°C. Single cell suspensions of each strain were prepared as previously described with slight modifications. Briefly, mycobacterial cells grown in OADC-supplemented 7H9 broth were harvested by centrifugation at 10,000 × g for 20 min and washed three times in phosphate-buffered saline (PBS) (pH 7.2). The pellets were homogenized using an overhead stirrer (Wheaton Instrument, Millville, NJ) for 1 min on ice to minimize bacterial clumping. The homogenized mycobacterial cells were passed through an 8 μm filter (Millipore Corp., Bedford, MA). The predominant presence of single cells in the final preparation was confirmed by acid-fast staining. The seed lots of each strain were stored in small aliquots at -80°C until use. The CFUs per 1 ml of the seed lots were measured by a viable counting assay on 7H10 agar plates.

Mtb H37Rv (ATCC 27294) and H37Ra (ATCC 25177) were purchased from the American Type Culture Collection (ATCC) (Manassas, VA, USA). The Mtb K strain (from the Beijing lineage, which is predominant in Korea^{77 (link)}) was obtained from the strain collection of the Korean Institute of Tuberculosis (Osong, Chungchungbuk-do, Republic of Korea). The Mtb HN878, Erdman and M2 strains were obtained from the strain collection of the International Tuberculosis Research Center (Changwon, Gyeongsangnam-do, Republic of Korea). The BCG vaccine (Pasteur 1173P2) was kindly provided by Dr. Brosch from the Pasteur Institute (Paris, France). All Mtb strains used in this study were cultured and prepared as previously described^{78 (link)}.