Anthropometrics and metabolic data were evaluated at the beginning and after the 8-week dietary intervention period. Blood samples were collected at fasting and 60, 120, 180, and 240 min after the test meal to measure plasma triglycerides, total cholesterol, and HDL cholesterol. Plasma glucose and lipids [total cholesterol, high-density lipoprotein (HDL) cholesterol, and triglyceride concentrations] were detected via enzymatic colorimetric methods (Roche Diagnostics, Milan, Italy, and ABX Diagnostics, Montpellier, France) using an ABX Pentra 400 (HORIBA Medical, Montpellier, France). HbA1c was measured via HPLC (Agilent HPLC 1200, Santa Clara, CA, USA). Plasma insulin concentrations were measured via ELISA (DIA-source ImmunoAssay S.A., Nivelles, Belgium) using a Triturus Analyzer (Diagnostic Grifols, S.A., Barcelona, Spain). The LDL cholesterol (Friedewald formula [23 (link)]) and non-HDL cholesterol (total cholesterol-HDL cholesterol values) concentrations were calculated. The homeostatic model assessment of insulin resistance (HOMA-IR) was calculated using the following formula: fasting glucose (mmol/L)·fasting insulin (pmol/L)/22.
Triturus analyzer
Manufactured by Grifols
Sourced in Belgium
The Triturus Analyzer is a laboratory instrument designed for automated immunoassay processing. It is capable of performing a variety of immunoassay tests, including enzyme-linked immunosorbent assays (ELISA) and chemiluminescent immunoassays (CLIA).
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using triturus analyzer
1
Metabolic and Lipid Profiles Assessment
2
Comprehensive Metabolic Profiling Protocol
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Plasma cholesterol, triglyceride, and glucose concentrations were assayed by enzymatic colorimetric methods (Roche Diagnostics, Milan, Italy, and ABX Diagnostics, Montpellier, France) on an ABX Pentra 400 (HORIBA Medical, Montpellier, France). High-density lipoprotein (HDL) cholesterol was isolated from plasma by the phosphotungstic acid/magnesium chloride precipitation method. LDL cholesterol was calculated using the Friedewald formula. Plasma insulin concentrations were measured by ELISA (DIA-source ImmunoAssay S.A., Nivelles, Belgium) on a Triturus Analyzer (Diagnostic Grifols, S.A., Barcelona, Spain). The homeostatic model assessment of insulin resistance (HOMA-IR) was calculated using the following formula: fasting glucose (mmol/L)×fasting insulin (pmol/L)/22.
Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (γ-GT) activities were determined by the Vista 1500 automated System (Siemens Healthcare Diagnostics Inc., Tarrytown, NY, USA). HbA1c was determined by the VARIANT II (Bio-Rad, Hercules, CA, USA) high-performance liquid chromatography (HPLC) system.
Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (γ-GT) activities were determined by the Vista 1500 automated System (Siemens Healthcare Diagnostics Inc., Tarrytown, NY, USA). HbA1c was determined by the VARIANT II (Bio-Rad, Hercules, CA, USA) high-performance liquid chromatography (HPLC) system.
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