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Microvette cb300 lh k2e tubes

Manufactured by Sarstedt

The Microvette CB300 LH K2E tubes are laboratory equipment used for the collection and storage of blood samples. These tubes contain the anticoagulant K2-EDTA, which helps prevent blood clotting during the collection process. The tubes have a volume capacity of 300 microliters and are designed for centrifugation, enabling the separation of blood components for analysis.

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2 protocols using microvette cb300 lh k2e tubes

1

Plasma NEFA and TG Quantification

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Blood was collected from the tail vein of mice in Microvette CB300 LH K2E tubes (Sarstedt). Plasma NEFA and TG levels were measured by colorimetrical analyses using the WAKO NEFA analysis kit (NEFA-HR(2); Wako Pure Chemical Industries) and the Infinity™ triglycerides kit, (Thermo Fisher Scientific), respectively.
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2

Blood Lipid Analysis in Mice

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Blood was collected from the tail veins of the mice in Microvette CB300 LH K2E tubes (Sarstedt). For retro-orbital bleeding, mice were first anesthetized with isoflurane. Blood was collected to Fisherbrand Micro Blood Collecting Tubes, Natelson. Plasma was analyzed for cholesterol using Infinity Cholesterol Liquid Stable Reagent (Thermo Fisher Scientific, TR13421), triglycerides using Infinity Triglycerides Liquid Stable Reagent (Thermo Fisher Scientific, TR22421), non-esterified fatty acids using a Free Fatty Acid Kit (Wako, HR series NEFA-HR(2)), and glycerol using Free Glycerol Reagent (Sigma, F6428) according to the manufacturer's instructions.
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