Log In Sign up
The largest database of trusted experimental protocols

P24g 1.5 13 f

Manufactured by MatTek
Visit Supplier

The P24G-1.5-13-F is a laboratory equipment product manufactured by MatTek. It is a compact device designed for specific technical functions within a laboratory setting. The core function of this equipment is to perform precise measurements and analyses, but a detailed description cannot be provided while maintaining an unbiased and purely factual approach.

Automatically generated - may contain errors

Lab products found in correlation

Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Sodium pyruvate, by Thermo Fisher Scientific (2 mentions) P35g 1.5 14 c, by MatTek (2 mentions) Fugene hd, by Promega (2 mentions) Efficient navigation zen , by Zeiss (2 mentions) Zen software, by Zeiss (2 mentions) Nis elements ar, by Nikon (2 mentions) Ti e inverted microscope, by Nikon (2 mentions) Mem non essential amino acid, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Merck Group (1 mentions) Mem vitamin solution, by Thermo Fisher Scientific (1 mentions) Poly d lysine, by BD (1 mentions) Bfgf2, by Thermo Fisher Scientific (1 mentions) Glutamine, by Thermo Fisher Scientific (1 mentions) N acetyl l cysteine, by Merck Group (1 mentions) P6407, by Merck Group (1 mentions)

9 protocols using p24g 1.5 13 f

1

Cell culture protocol for X1(FS) and SiRNeoblasts

Check if the same lab product or an alternative is used in the 5 most similar protocols
Dishes and multi-well plates were pre-coated with poly-D-lysine (50 μg/mL, BD Biosciences). If not specified, 1 X 104 X1(FS) cells or SiRNeoblasts were cultured in 50 μL indicated culture medium containing 5% Fetal Bovine Serum (Sigma-Aldrich, F4135) per well in 384-well plates (Greiner bio-one, 781090) at 22°C with 5% CO2 or ambient atmosphere.
For time-lapse imaging experiments, 6 X 105 X1(FS) cells were pre-loaded in the center of a PDL-coated MatTek dish with 100 μL medium for 30 min and then added in either 5 mL of the indicated culture medium per well of 6 cm dishes (MatTek, P35G-1.5-14-C) or 1 mL of the indicated culture medium per well of 24-well plates (MatTek, P24G-1.5-13-F).
For transfection experiments, if not specified, 2 × 105 bulk cells, sorted live cells, or SiRNeoblasts were cultured in 96-well plates with 225 μL medium (with or without supplements) per well. Supplements containing 100 X MEM Vitamin Solution (Gibco, 11120-052), 100 X MEM Non-Essential Amino Acid (Gibco, 11140-050), 100 mM Sodium Pyruvate (Gibco, 11360-070), and Penicillin Streptomycin (Gibco, 15140-122) were added to the basal medium at the 1:100 dilution.
Lei K., Zhang W., Chen J., McKinney S.A., Ross E.J., Lee H.C, & Sánchez Alvarado A. (2023). Pluripotency retention and exogenous mRNA introduction in planarian stem cells in culture. iScience, 26(2), 106001.
+ Open protocol
+ Expand
2

Dynamic Biosensor Imaging in HEK293T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293T cells were transfected with Tornado plasmids encoding either the circular SAM biosensor with transducer 1 or circular Broccoli. Two days later, these cells were subcultured onto coated glass bottom plates (MatTek Corporation P24G-1.5–13-F) and imaged the next day. Using live cell conditions described above, we imaged cells before and for 3 h after adding cycloleucine (Sigma Aldrich A48105) to 100 mM at 5 min intervals. Then, we withdrew cycloleucine and continued to image cells every 5 min for 3 additional h. ImageJ59 (link) was used for processing images and for measure the total cell fluorescence at each time point.
Litke J.L, & Jaffrey S.R. (2019). Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts. Nature biotechnology, 37(6), 667-675.
+ Open protocol
+ Expand
3

Quantifying Transfected Cell Growth

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293T cells were independently transfected with plasmids encoding Broccoli using Tornado or encoding mCherry with FuGENE HD (Promega 2311). 2 d after transfection, cells were subcultured 1:4 and 1:8 onto glass-bottom plates (MatTek Corporation P24G-1.5–13-F). The next day, fluorescent cells in each 1:4 subculture were counted in 4 fields, as well as the number of Hoechst-stained cells Hoechst. After 2 d, the 1:8 subcultures were imaged in the same way. We calculated the number of non-fluorescent cells in each culture and then quantified doubling times of fluorescent and non-fluorescent cells according to the formula for exponential growth.
Litke J.L, & Jaffrey S.R. (2019). Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts. Nature biotechnology, 37(6), 667-675.
+ Open protocol
+ Expand
4

Live-cell Imaging of Amino Acid Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded on glass-bottom plates (P24G-1.5-13-F; Mattek) and treated in fresh culture media the next day. For amino acid-free conditions, cells were washed with PBS twice prior to treatment. Imaging was performed in live-cell incubation chambers maintained at 37°C and 5% CO2. Images were acquired every 5 to 30 min for 24-48 hours using a Nikon Ti-E inverted microscope attached to a CoolSNAP charge-coupled device camera (Photometrics) and NIS Elements AR software (Nikon, 3.22.15). For experiments with ML162, time-lapse imaging was performed on plastic tissue culture plates (3527; Corning) on a Zeiss microscope using ZEN software (Zeiss). Images were quantified manually in NIS Elements AR (Nikon) or ZEN (Zeiss) and processed using ImageJ (2.0.0) and Adobe Photoshop (CS6 13.0.5).
Riegman M., Sagie L., Galed C., Levin T., Steinberg N., Dixon S.J., Wiesner U., Bradbury M.S., Niethammer P., Zaritsky A, & Overholtzer M. (2020). Ferroptosis occurs through an osmotic mechanism and propagates independently of cell rupture.. Nature cell biology, 22(9), 1042-1048.
+ Open protocol
+ Expand
5

Mouse Cortical Cell Culture Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
The E14.5 mouse cortices were dissected out and dissociated by trituration in Hanks’ balanced salt solution (HBSS) (Mediatech). Cells were re-suspended in culture medium DMEM with 4.5 g/l Glucose (Mediatech), glutamine (Gibco), penicillin/streptomycin (Gibco), sodium pyruvate (Gibco), 1 mM N-acetyl-l-cysteine (Sigma), B27 (Invitrogen), N2 (Invitrogen), and 10 ng/ml basic fibroblast growth factor-2 (bFGF2; Gibco), and were plated down into poly-d-lysine (P6407, Sigma)-coated 24-well glass-bottom plate (P24G-1.5-13-F, MatTek) at 2 × 105 cell per well. After 20 h of culture, cells were processed for antibody staining. The experiment was repeated twice.
Geng A., Qiu R., Murai K., Liu J., Wu X., Zhang H., Farhoodi H., Duong N., Jiang M., Yee J.K., Tsark W, & Lu Q. (2018). KIF20A/MKLP2 regulates the division modes of neural progenitor cells during cortical development. Nature Communications, 9, 2707.
+ Open protocol
+ Expand
6

Live-Cell Imaging of Transfected Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Transfected cells were seeded on FN1-coated 35-mm glass dishes (P35G-1.5-14-C, MatTek Corporation) for TIRF and epifluorescence experiments, or on FN1-coated 24 multi-well glass plate (P24G-1.5-13-F; MatTek Corporation) for phase-contrast experiments. 24 h after transfection, live-cell imaging was performed in serum-free Leibovitz’s L-15 medium supplemented with 1% D-glucose, 1% antibiotic-antimycotic, and 1% sodium pyruvate (TIRF and epifluorescence) or in serum-free DMEM supplemented with 1% antibiotic-antimycotic and 1% sodium pyruvate (phase contrast).
Campisi D., Desrues L., Dembélé K.P., Mutel A., Parment R., Gandolfo P., Castel H, & Morin F. (2022). The core autophagy protein ATG9A controls dynamics of cell protrusions and directed migration. The Journal of Cell Biology, 221(3), e202106014.
+ Open protocol
+ Expand
7

Dynamic Biosensor Imaging in HEK293T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293T cells were transfected with Tornado plasmids encoding either the circular SAM biosensor with transducer 1 or circular Broccoli. Two days later, these cells were subcultured onto coated glass bottom plates (MatTek Corporation P24G-1.5–13-F) and imaged the next day. Using live cell conditions described above, we imaged cells before and for 3 h after adding cycloleucine (Sigma Aldrich A48105) to 100 mM at 5 min intervals. Then, we withdrew cycloleucine and continued to image cells every 5 min for 3 additional h. ImageJ59 (link) was used for processing images and for measure the total cell fluorescence at each time point.
Litke J.L, & Jaffrey S.R. (2019). Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts. Nature biotechnology, 37(6), 667-675.
+ Open protocol
+ Expand
8

Quantifying Transfected Cell Growth

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293T cells were independently transfected with plasmids encoding Broccoli using Tornado or encoding mCherry with FuGENE HD (Promega 2311). 2 d after transfection, cells were subcultured 1:4 and 1:8 onto glass-bottom plates (MatTek Corporation P24G-1.5–13-F). The next day, fluorescent cells in each 1:4 subculture were counted in 4 fields, as well as the number of Hoechst-stained cells Hoechst. After 2 d, the 1:8 subcultures were imaged in the same way. We calculated the number of non-fluorescent cells in each culture and then quantified doubling times of fluorescent and non-fluorescent cells according to the formula for exponential growth.
Litke J.L, & Jaffrey S.R. (2019). Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts. Nature biotechnology, 37(6), 667-675.
+ Open protocol
+ Expand
9

Live-cell Imaging of Amino Acid Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded on glass-bottom plates (P24G-1.5-13-F; Mattek) and treated in fresh culture media the next day. For amino acid-free conditions, cells were washed with PBS twice prior to treatment. Imaging was performed in live-cell incubation chambers maintained at 37°C and 5% CO2. Images were acquired every 5 to 30 min for 24-48 hours using a Nikon Ti-E inverted microscope attached to a CoolSNAP charge-coupled device camera (Photometrics) and NIS Elements AR software (Nikon, 3.22.15). For experiments with ML162, time-lapse imaging was performed on plastic tissue culture plates (3527; Corning) on a Zeiss microscope using ZEN software (Zeiss). Images were quantified manually in NIS Elements AR (Nikon) or ZEN (Zeiss) and processed using ImageJ (2.0.0) and Adobe Photoshop (CS6 13.0.5).
Riegman M., Sagie L., Galed C., Levin T., Steinberg N., Dixon S.J., Wiesner U., Bradbury M.S., Niethammer P., Zaritsky A, & Overholtzer M. (2020). Ferroptosis occurs through an osmotic mechanism and propagates independently of cell rupture.. Nature cell biology, 22(9), 1042-1048.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!