Optical rotations were measured on a JASCO P-2200 polarimeter in a 0.5 dm cell. The UV spectra were obtained with a Shimadzu UV-160 spectrophotometer. The IR spectra were measured on a JASCO FT/IR-4100 Fourier transform infrared spectrometer by the KBr disk method. The 1H and 13C NMR spectra were measured on a JEOL ECA-500 spectrometer with the deuterated solvent as the internal reference, and the chemical shifts are expressed in δ (ppm). HRFABMS and HRESITOFMS were conducted using a JEOL JMS-700 MStation and a JEOL JMST100LP AccuTOF LC-plus mass spectrometer, respectively. Diaion HP-20 (Mitsubishi Chemical Corporation, Tokyo, Japan) was used for column chromatography. RP-HPLC was performed on a Waters 515 HPLC pump equipped with a Shodex RI-101 differential refractometer detector and a JASCO UV-970 intelligent UV–vis detector. An RP-C18 silica gel column (YMC-Pack Pro C18, 150 × 20 mm) was used at a flow rate of 5.0 mL/min. Sep-Pak C18 and Sep-Pak silica cartridges were purchased from Waters (Milford, MA, U.S.A.). The purity of the tested compounds (>95%) was confirmed by HPLC-PDA analysis and 1H-NMR spectroscopic analysis. LCMS analysis was conducted on a Shimadzu LCMS-8040 Triple Quadrupole LC/MS/MS mass spectrometer. Solvents for LCMS analysis and synthesis were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).
Lcms 8040 triple quadrupole lc ms ms mass spectrometer
Manufactured by Shimadzu
Sourced in Japan
The LCMS-8040 is a triple quadrupole LC/MS/MS mass spectrometer manufactured by Shimadzu. It is designed for high-throughput quantitative analysis, providing fast scanning and high sensitivity. The instrument utilizes triple quadrupole technology to perform tandem mass spectrometry (MS/MS) for the detection and quantification of target analytes in complex samples.
Automatically generated - may contain errors
Lab products found in correlation
Jms 700 mstation, by JEOL (1 mentions)
Sep pak silica cartridge, by Waters Corporation (1 mentions)
Sep pak c18, by Waters Corporation (1 mentions)
Uv 160 spectrophotometer, by Shimadzu (1 mentions)
Eca 500 spectrometer, by JEOL (1 mentions)
P 2200 polarimeter, by Jasco (1 mentions)
Ft ir 4100 fourier transform infrared spectrometer, by Jasco (1 mentions)
Diaion hp 20, by Mitsubishi Chemical Corporation (1 mentions)
Triart c18 column, by YMC (1 mentions)
3 protocols using lcms 8040 triple quadrupole lc ms ms mass spectrometer
1
Structural Characterization of Compounds
2
LC-MS Analysis of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LC-MS analysis was performed using an LCMS-8040 triple quadrupole LC/MS/MS mass spectrometer (Shimadzu Co., Ltd., Kyoto, Japan). HPLC was performed on a YMC-Triart C18 column (3.0 μm, 150 × 20 mm I.D.) maintained at 35 °C. The flow rate was 0.2 mL/min. The mobile phase was composed of A (0.1% HCOOH in H2O) and B (0.1% HCOOH in MeCN) with a gradient elution: 0–40 min, 20–70% B; 40–50 min, 100% B. The column was equilibrated for 15 min under the initial conditions. The injection volume was 4 µL for the qualitative analysis.
The mass spectrometer was operated in both positive- and negative-ion modes in the range of m/z 100 to 1600. The ESI parameters were as follows: interface voltage, 4.5 kV in the positive-ion mode and −3.5 kV in the negative-ion mode; dry gas, 15 L/min; and dry temperature, 350 °C.
The mass spectrometer was operated in both positive- and negative-ion modes in the range of m/z 100 to 1600. The ESI parameters were as follows: interface voltage, 4.5 kV in the positive-ion mode and −3.5 kV in the negative-ion mode; dry gas, 15 L/min; and dry temperature, 350 °C.
3
LC-ESI-MS Analysis of YNCRG Extract
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
LC-ESI-MS analysis was carried out using an LCMS-8040 triple quadrupole LC/MS/MS mass spectrometer (Shimadzu Co., Ltd., Kyoto, Japan) with a YMC-Triart C18 column (3.0 µm, 150 × 20 mm I.D.). The flow rate was 0.2 mL/min, and the column temperature was maintained at 35 ºC. The mobile phase was composed of A (0.1% formic acid in H2O) and B (0.1% formic acid in acetonitrile) with a gradient as follows: 10% B in 0-3 min, 10-50% B in 3-10 min, 50-90% B in 10-40 min, 100% B in 40-50 min. The mass spectrometer was operated in both positive-and negative ion modes with the full scan mode in the range of 100-1600 m/z. The optimized instrument settings were as follows: interface voltage, 4.5 kV in the positive-ion mode, and -3.5 kV in the negative ion mode; collision voltage, 15 V; dry gas, 15 L/min, and dry temperature 350 °C. YNCRG (0.5 g) was ultrasonically extracted with MeOH (20 mL) at room temperature for 1 h. The extracted solutions (1 mL) were purified using a Sep-Pak C18 plus short cartridge and diluted to 10 mL. The solutions were filtered through a 0.45 µm syringe filter. A volume of 2 µL was injected for LC-ESI-MS analysis [31] .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!