Renal tissues levels of mouse IL-17A, IFN-γ (MULTISCIENCES: EK217/2-AW1, EK280/3-AW1), IL-6, IL-4 and IL-10 (Elabscience: E-EL-M0044c, E-EL-M0043c, E-EL-M0046c) were quantified by using an ELISA method according to the instructions. Renal tissue lysates were extracted according to the following formula: 50 mmol/L Tris–HCl, 0.2% Triton X-100, 2 mmol/L EDTA, 150 mol/LNaCl, 2 mmol/L EGTA, 0.3%IGEPAL, 10 μl/ml proteinase inhibitors cocktail, 10 μl/ml PMSF, and 10 μl/ml orthovanadate (21 (link)).
E el m0046c
Manufactured by Elabscience
Sourced in China
The E-EL-M0046c is a laboratory equipment product from Elabscience. It is a core function device, but a detailed description cannot be provided while maintaining an unbiased and factual approach. More information may be available from the manufacturer.
Automatically generated - may contain errors
Lab products found in correlation
E el m0044c, by Elabscience (5 mentions)
E el m0037c, by Elabscience (4 mentions)
E el m0043c, by Elabscience (2 mentions)
E el m0049c, by Elabscience (2 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
E el m3063, by Elabscience (1 mentions)
E msel m0002, by Elabscience (1 mentions)
6 protocols using e el m0046c
1
Quantifying Renal Cytokine Levels in Mice
2
Quantifying Inflammatory Cytokines in Mice
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At the end of the experiment, blood was obtained from the mice and their serum was collected following centrifugation at 3000 rpm for 30 min. The ELISA kits for measuring IL‐1β (E‐EL‐M0037c), IL‐6 (E‐EL‐M0044c), TNF‐α (E‐EL‐M3063), IL‐10 (E‐EL‐M0046c) and IL‐4 (E‐EL‐M0043c) were purchased from Elabscience (Wuhan, China). The levels of IL‐1β, IL‐6, TNF‐α, IL‐10 and IL‐4 in the mouse serum were quantified using the respective ELISA kits according to the manufacturer's instructions. The absorbance was determined on a microplate reader at OD450 nm.
3
Cytokine Profiling in Tissue and Serum
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The brain tissues were homogenized in PBS to obtain the tissue fluid. Blood samples were placed in sterile tubes for 1 h at RT and then centrifuged at 2000×g for 10 min at 4° C to obtain serum. ELISA kits were used to detect the concentrations of TNF-α (#E-EL-M0049c, Elabscience, Wuhan, China), IL-1β (#E-EL-M0037c, Elabscience), IL-6 (#E-EL-M0044c, Elabscience), and IL-10 (#E-EL-M0046c, Elabscience) according to the manufacturer’s instructions.
4
Quantifying Inflammatory Cytokines in Mouse Brains
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The mouse brains were homogenized with 8 M urea lysate, and the lysate was left standing at 4°C for 30 minutes and centrifuged at 12000 rpm in a high-speed centrifuge for 30 minutes, and the supernatant was taken for use. The BCA protein assay kit (Thermo Fisher, NJ, USA) was used to measure the concentration of extracted proteins. The supernatant was analyzed by using mouse IL-10, IL-6, and TNF-α ELISA kits according to the manufacturer's instructions (E-EL-M0046c, E-EL-M0044c, and E-EL-M0049c, respectively, Elabscience). The concentrations of IL-10, IL-6, and TNF-α were determined by comparison with the standard curve.
5
Cytokine Profiling via ELISA
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Serum and cell supernatants were analyzed using mouse IL-6, IL-10, and IL-1β ELISA kit (#E-EL-M0044c, #E-EL-M0046c, and #E-EL-M0037c; Elabscience Biotechnology). ELISA analysis was performed according to the manufacturer’s instructions.
6
Evaluating Inflammatory Markers in BV-2 Cells
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Serum insulin, TNF-α, IL-1β and IL-6 levels were analyzed at 12 weeks after treatment via ELISA following the instructions. The blood samples were detected by (insulin) EILISA kit (DL-INS-Ra; DLdevelop), (IL-1β) ELISA Kit (DL-IL1b-Ra; DLdevelop), (IL-6) ELISA Kit (DL-IL6-Ra; DLdevelop) and (TNF-α) ELISA Kit (DL-TNFα-Ra; DLdevelop). BV-2 cells were seeded at 5 × 104/mL and stimulated with glucose (50 mM) in 96-well plate and then treated with LEV, LEV combined with anisomycin. The medium concentrations of IL-1β, TNF-α, and IL-10 were detected using ELISA kits (E-EL-M0037c, E-EL-M0046c, E-MSEL-M0002, respectively; Elabscience). The absorbance value of the mixture was measured at 450 nm.
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