Lactic dehydrogenase ldh kit

Poly(hydroxybuyrate) (PHB) was purchased from Goodfellow (Huntington, England). Fiber-grade polylactic acid (PLA) (NatureWorks 6202D) was purchased from NatureWorks (Minnetonka, MN). Glutathione was purchased from GoldBio (St. Louis, MO). Acetone was purchased from VWR (Radnor, PA). Ethylenediaminetetraacetic acid (EDTA), Dulbecco’s modified Eagle’s medium (DMEM), sodium nitrite, DAPI, and the CCK-8 kit were purchased from Sigma Aldrich (St. Louis, MO). Penicillin-Streptomycin and fetal bovine serum were purchased from Gibco-Life Technologies (Grand Island, NY). HCl was purchased from Fisher Scientific (Hampton, NH). Hexafluoro-2-propanol (HFIP) was purchased from Oakwood Scientific (Estill, SC). Phosphate buffered saline (PBS), pH 7.4, composed of 138 mM NaCl, 2.7 mM KCl, and 10 mM sodium phosphate, was used for all in vitro experiments. The lactic dehydrogenase (LDH) kit was purchased from Roche Life Sciences (Indianapolis, IN). The bacteria strain S. aureus (ATCC 5538) and NIH/3T3 mouse fibroblasts (ATCC 1658) were received from American Type Culture Collection (Manassas, VA). Alexa Fluor 488 phalloidin was purchased from Thermo Fisher Scientific (Waltham, MA).

N-Acetyl-D-penicillamine (NAP) (≥ 99.0 % purity), tetrahydrofuran (THF, ≥ 98.0 %), sodium nitrite (NaNO_2, ≥ 99.0 %), ethylenediaminetetraacetic acid (EDTA, ≥ 99 %) and hydrochloric acid were purchased from Sigma Aldrich (St. Louis, MO 63103). CarboSil-2080A™ (hereon as CarboSil) was purchased from DSM (Berkeley, CA). Teflon™ PTFE emulsion, DISP 30 LX, was purchased from Fuel Cell Earth (Woburn, MA); Phosphatebuffered saline (PBS), pH 7.4, containing 138 mM NaCl, 2.7 mM KCl, and 0.01 M sodium phosphate. Dulbecco’s modified Eagle’s medium (DMEM) and trypsin-EDTA were purchased from Corning (Manassas, VA 20109). The Cell Counting Kit-8 (CCK-8) was purchased from Sigma Aldrich (St. Louis, MO 63103). Penicillin-Streptomycin (Pen-Strep) and fetal bovine serum (FBS) was obtained from Gibco-Life Technologies (Grand Island, NY 14072). The bacterial strains S. aureus (ATCC 6538) and E. coli (ATCC 25922) were purchased from American Type Culture Collection (ATCC). LB broth was obtained from Fisher Bioreagents (Fair Lawn, NJ). LB Agar was purchased from Difco Laboratories Inc (Detroit, MI). The lactic dehydrogenase (LDH) kit was purchased from Roche Life Sciences (Indianapolis, IN). Hexamethyldisilazane (HMDS, ≥ 99 % purity) was obtained from Sigma Aldrich (St. Louis, MO 63103)

N-Acetyl-D-penicillamine (NAP), tetrahydrofuran (THF), sodium nitrite (NaNO₂), ethylenediaminetetraacetic acid (EDTA), hydrochloric acid, and selenium powder (−100 mesh) were purchased from Sigma Aldrich (St. Louis, MO 63103). CarboSil-2080A™ (CarboSil) was purchased from DSM (Berkeley, CA). Phosphate-buffered saline (PBS), pH 7.4, used for in vitro experiments contained 138 mM NaCl, 2.7 mM KCl, and 10 mM sodium phosphate. Dulbecco’s modified Eagle’s medium (DMEM) and trypsin-EDTA were purchased from Corning (Manassas, VA 20109). The Cell Counting Kit-8 (CCK-8) was purchased from Sigma Aldrich (St. Louis, MO 63103). Penicillin-Streptomycin (Pen-Strep) and fetal bovine serum (FBS) was obtained from Gibco-Life Technologies (Grand Island, NY 14072). The bacterial strains S. aureus (ATCC 6538) and E. coli (ATCC 11303) were purchased from American Type Culture Collection (ATCC). LB broth was obtained from Fisher Bioreagents (Fair Lawn, NJ). LB Agar was purchased from Difco Laboratories Inc (Detroit, MI). Reagents used for NO generation. The lactic dehydrogenase (LDH) kit was purchased from Roche Life Sciences (Indianapolis, IN). Glutathione reduced was purchased from Goldbio (St. Louis, MO).