Human epithelial ovarian cancer cell lines SKOV3 and OVCAR3 were purchased from China Typical Culture Preservation Center (Wuhan, China), and 10% FBS (GIBCO BRL, USA) was supplemented in Dulbecco modified Eagle medium (DMEM) (GIBCO BRL, USA). For the overexpression of miRNA-335, SKOV3 and OVCAR3 cells were transfected with miR-335 mimic (GenePharma, China). For the overexpression of BCL2L2, SKOV3 cells were transfected with the BCL2L2 expression construct, which has become insensitive through the mutation of 3′UTR (GenePharma, USA) of miR-335. Lipofectamine was used in all transfections Gamma 2000 reagents (Invitrogen life technologies, USA) which are carried out according to the manufacturer’s instructions.
Skov3
Manufactured by GenePharma
Sourced in China
The SKOV3 is a laboratory cell line derived from human ovarian adenocarcinoma. It is commonly used in cancer research and drug development studies.
Automatically generated - may contain errors
Lab products found in correlation
Skov3, by American Type Culture Collection (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Merck Group (2 mentions)
Si nc, by GenePharma (2 mentions)
Caov3, by American Type Culture Collection (2 mentions)
Ovcar3, by American Type Culture Collection (2 mentions)
Nc sirna, by GenePharma (1 mentions)
Ovcar3, by GenePharma (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Sw626, by American Type Culture Collection (1 mentions)
Sh kcnq1ot1, by GenePharma (1 mentions)
Penicillin streptomycin, by Merck Group (1 mentions)
Sh nc, by GenePharma (1 mentions)
Skov3, by Thermo Fisher Scientific (1 mentions)
Igrov 1, by Thermo Fisher Scientific (1 mentions)
Si ccat2, by GenePharma (1 mentions)
Ovcar8, by Thermo Fisher Scientific (1 mentions)
A2780, by Thermo Fisher Scientific (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
A2780, by American Type Culture Collection (1 mentions)
5 protocols using skov3
1
Overexpressing miRNA-335 and BCL2L2 in Ovarian Cancer
2
Silencing LSINCT5 in Ovarian Cancer Cells
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Human ovarian cancer cell lines (SKOV3, OVCAR-3 and 3AO) were obtained from the Chongqing Key Laboratory of Oncology and were cultured in RPMI-1640 media (Wuhan Boster Biological Technology, Ltd., Wuhan, China) containing 10% foetal bovine serum (FBS) (Wuhan Boster Biological Technology, Ltd.), penicillin and streptomycin at 37°C in a 5% CO2 incubator. The LSINCT5-specific small interfering RNAs (siRNAs) and negative control (NC) siRNAs (GenePharma, Shanghai, China) were transfected to SKOV3 cells by following the manufacturers manual. The target sequences for the LSINCT5 siRNAs were 5′-CCAGCUACAAACCUCUGAATT-3′ and 5′-UUCAGAGGUUUGUAGCUGGTT-3′ (LSINCT5-siRNA-1); 5′-GAACUGGAUUAGUGUUAAATT-3′ and 5′-UUUAACACUAAUCCAGUUCTT-3′ (LSINCT5-siRNA-2); 5′-CCUCCAAACACAUGGAUAATT-3′ and 5′-UUAUCCAUGUGUUUGGAGGTT-3′ (LSINCT5-siRNA-3).
3
Ovarian Cancer Cell Culture and Knockdown
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Human ovarian surface epithelial cells (IOSE-80#, LMAIBio, Shanghai, China), OC A2780 cells (CL-0013#, Procell, Wuhan, China), Anglne (CL-0024#, Procell), SKOV3 (HTB-77#, ATCC, Manassas, VA, USA), SW626 (HTB-78#, ATCC), COV362 (07071910#, ECACC, European Collection of Authenticated Cell Cultures), CAOV3 (HTB-753#, ATCC) and OVCAR-3 (HTB-161#, ATCC) cells were cultured in a humidified incubator with 5% CO2 at 37 °C. Cells were maintained in RPMI-1640 medium (Sigma-Aldrich, St. Louis, MO, USA) mixed with 10% FBS (Gibco, Gran Island, NY, USA) and 1% penicillin–streptomycin (Sigma-Aldrich). Lentiviral-based shRNA targeting KCNQ1OT1 (sh-KCNQ1OT1; GenePharma, Shanghai, China) or EIF2B5 (sh-EIF2B5; GenePharma) was stably transfected into SKOV3 and SW626 cells to silence KCNQ1OT1 or EIF2B5 expression, with sh-NC (GenePharma) as negative control.
4
Silencing CCAT2 in Ovarian Cancer Cells
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Standard ovarian cancer cell lines (SKOV3, IGROV1, A2780, and OVCAR3) and a normal human ovarian surface epithelial cell line (HOSE 6.3) were obtained from the American Type Culture Collection. Ovarian cancer cell lines were routinely cultured in DMEM (OVCAR-8 and SKOV-3) or RPMI 1640 (A2780 and IGROV-1), supplemented with 10 % fetal bovine serum (FBS; Gibco, Grand Island, NY, USA), 100 U/ml penicillin sodium, and 100 mg/ml streptomycin sulfate. HOSE 6.3 cells were maintained in MCDB Medium 199 (1:1, v/v) containing 10 % FBS [18 (link)]. All cells were cultured in a humidified atmosphere containing 5 % CO2 at 37 °C.
SKOV3 cells were transfected with siRNA (50 nM) targeting CCAT2 (si-CCAT2) or a scrambled negative control (si-NC) (GenePharma, China) using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol. The sequence selected for CCAT2 siRNA was 5’-AGGTGTAGCCAGAGTTAAT-3’ [13 (link)]. Cells were cultured for 48 h after transfection, and harvested to determine transfection efficiency by qRT-PCR.
SKOV3 cells were transfected with siRNA (50 nM) targeting CCAT2 (si-CCAT2) or a scrambled negative control (si-NC) (GenePharma, China) using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol. The sequence selected for CCAT2 siRNA was 5’-AGGTGTAGCCAGAGTTAAT-3’ [13 (link)]. Cells were cultured for 48 h after transfection, and harvested to determine transfection efficiency by qRT-PCR.
5
Silencing DLGAP5 in Human OC Cells
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Two human OC cell lines, including SKOV3 and CAOV3 were purchased from the American Type Culture Collection and routinely cultured in RPMI-1640 medium (Sigma-Aldrich; Merck KGaA) supplemented with 10% FBS (Sigma-Aldrich; Merck KGaA) at 37˚C in a humidified incubator containing 5% CO2. For DLGAP5 knockdown, SKOV3 and CAOV3 cell were seeded into six-well plates at a density of 2x105 cells per well and transfected with 50 nM small interfering (si)RNA targeting DLGAP5 (si-DLGAP5-1: 5'-ATTCAAACCACATCAGCCTGC-3' or si-DLGAP5-2: 5'-TTGACCGGAGAAGATAGAGGA-3') or negative control (si-NC: 5'-GAAACACAGCCTGTACGCCTG-3') (all chemically synthesized from Shanghai GenePharma Co., Ltd.) using Lipofectamine® 2000 reagent (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. Subsequent experiments were performed 48 h post transfection.
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