Myricetin, Hoechst Staining Kit (G3680) and resazurin were purchased from Solarbio Life Sciences (Beijing, China). 3-MA (S2767) was purchased from Selleck Chemicals (Houston, TX, USA). The primary antibodies included Bax (5023S), Bcl-2 (15071S), Microtubule-associated protein1 light chain 3 (LC-3) I/II (12741S), Beclin-1 (3495S), Phosphatidyl inositide 3-kinase (PI3K) (13666S), phospho-PI3K (4228S), phospho-Akt (4060S), Akt (2920S), Mammalian target of rapamycin (mTOR) (2983S), phospho-mTOR (5536S) were purchased from Cell Signaling (MA, USA). β-actin (60008–1) was purchased from ProteinTech Group (Wuhan, China). Horseradish peroxidase-conjugated secondary antibodies (ZB-2301, ZB-2305) were purchased from ZSGB-Bio (Beijing, China).
Resazurin
Manufactured by Solarbio
Sourced in China
Resazurin is a redox-sensitive dye commonly used in cell viability and proliferation assays. It is a blue, non-fluorescent compound that is reduced to the pink, highly fluorescent compound resorufin in the presence of metabolically active cells. The degree of this color change and fluorescence intensity can be used to quantify cell viability and metabolic activity.
Automatically generated - may contain errors
Lab products found in correlation
Galanthamine, by Solarbio (2 mentions)
Tyrosinase, by Merck Group (2 mentions)
α glucosidase, by Merck Group (2 mentions)
Acarbose, by Solarbio (2 mentions)
Kojic acid, by Solarbio (2 mentions)
Formic acid, by Merck Group (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
Streptomycin, by Solarbio (2 mentions)
Folin ciocalteu reagent, by Solarbio (2 mentions)
Rutin, by Solarbio (2 mentions)
Gallic acid, by Solarbio (2 mentions)
3 ma s2767, by Selleck Chemicals (1 mentions)
Myricetin, by Solarbio (1 mentions)
Penicillin streptomycin, by Solarbio (1 mentions)
Trypsin edta, by Solarbio (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Fetal calf serum (fcs), by Merck Group (1 mentions)
Non essential amino acids (neaa), by Solarbio (1 mentions)
Rpmi 1640, by Solarbio (1 mentions)
Dulbecco modified eagle medium (dmem), by Solarbio (1 mentions)
4 protocols using resazurin
1
Myricetin Modulates Autophagy and Apoptosis Pathways
2
Cytotoxicity Assay Protocol
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RPMI1640, DMEM, Non-Essential Amino Acids, Penicillin/Streptomycin, Resazurin, and Trypsin-EDTA were procured from Solarbio (China Phosphate buffered saline (PBS), 5FU, FCS, and Dimethyl Sulfoxide (DMSO) were procured from Sigma Aldrich (Germany).
3
Comprehensive Biochemical Assays for Biological Evaluation
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Streptomycin, Folin-Ciocalteu reagent, resazurin, galanthamine, kojic acid, acarbose, rutin, and gallic acid were from Solarbio Life Sciences (Beijing, China). Sigma-Aldrich (Germany) provided BTCl (butyrylthiocholine chloride), ATCI (acetylthiocholine iodide), L-tyrosine, p-NPG (p-nitrophenyl-α-D-glucopyranoside), BChE (butyrylcholinesterase), AChE (acetylcholinesterase), tyrosinase, α-glucosidase, ABTS (2,2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid), DPPH (1,1-diphenyl-2-picrylhydrazyl), BHT (butylated hydroxytoluene), and ascorbic acid. Merck (Darmstadt, Germany) supplied a standard n-alkane (C7–C30) in the GC-MS test and formic acid and acetonitrile (LC/MS-grade) in the UHPLC-Q-Orbitrap-MS test. Aladdin (Shanghai, China) offered other solvents and chemicals in analytical purity.
4
Bioactive Compound Evaluation and Assays
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Gallic acid, rutin, streptomycin, cisplatin, acarbose, kojic acid, galanthamine, resazurin, and Folin-Ciocalteu reagent were obtained from Solarbio Life Sciences (Beijing, China). Ascorbic acid, butylated hydroxytoluene (BHT), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS), acridine orange (AO), ethidium bromide (EB), α-glucosidase, tyrosinase, acetylcholinesterase (AChE), butyrylcholinesterase (BChE), p-Nitrophenyl-α-D-glucopyranoside (p-NPG), L-tyrosine, acetylthiocholine iodide (ATCI), and butyrylthiocholine chloride (BTCl) were purchased from Sigma-Aldrich (Germany). Hoechst 33258 and Annexin V-FITC/PI Apoptosis Detection Kit were from Beyotime (Haimen, China). For GC-MS analysis, analytical standard n-alkanes (C8–C22) were from Merck (Darmstadt, Germany). For UPLC-MS analysis, LC/MS-grade acetonitrile, formic acid, and ultrapure water were supplied from Merck (Darmstadt, Germany). All other chemicals and solvents were of analytical grade and purchased from Aladdin (Shanghai, China).
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