Opti modified eagle s medium opti mem

RPMI1640 medium, Opti-modified Eagle’s medium (Opti-MEM), fetal bovine serum (FBS), penicillin, and streptomycin were purchased from Life Technologies, Inc. (Grand Island, NY, USA). Dimethyl sulfoxide (DMSO), RNase A, leupeptin, aprotinin, phenylmethylsulfonylfluoride, and Triton X-100 were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). The pCMV-Neo-Bam p53-R175H, pCMV-Neo-Bam p53-R248W, pCMV-Neo-Bam p53-R273H, and pCMV-Neo-Bam p53 wild-type were obtained from Addgene (Cambridge, MA, USA). The antibodies against anti-p53, anti-Rad21, anti-S1PR1, anti-THBS1, and anti-β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The protein G PLUS-agarose was also obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Roswell Park Memorial Institute (RPMI) 1640 medium, Opti-modified Eagle’s medium (Opti-MEM), fetal bovine serum (FBS), penicillin, and streptomycin were procured from Life Technologies, Inc. (Grand Island, NY, USA). First-strand cDNA synthesis kit was procured from Amersham Pharmacia Biotech (Oakville, ON, Canada). Easy Blue^® for RNA isolation and protein lysis kits were purchased from Intron Biotechnology (Seoul, South Korea). Neutralizing antibody for anti-CCL7 was purchased from Abcam (Cambridge, MA, USA). Human recombinant CCL7 was obtained from Perotech (Rocky Hill, NJ, USA). CCR3 inhibitor SB297006 was purchased from B&D Life Sciences (San Diego, CA, USA). MEK inhibitor PD98059, PI3K/Akt inhibitor LY294002, JNK inhibitor SP600125, p38 inhibitor SB203580, and matrix metalloproteinase (MMP)-9 inhibitor SC311437 were obtained from Calbiochem (San Diego, CA, USA). Transwell inserts with polyvinylpyrrolidone-free polycarbonate filter (8-μm pore) and BD Matrigel™ Basement Membrane Matrix were purchased from BD Biosciences (San Jose, CA, USA).