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Cd45r b220 pe

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CD45R/B220-PE is a fluorescent-labeled antibody that binds to the CD45R/B220 antigen, which is expressed on the surface of B cells. It is used in flow cytometry applications for the identification and enumeration of B cells in research samples.

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Lab products found in correlation

Nk1.1 pe, by BD (2 mentions) Cd49b pe, by BD (2 mentions) Cd90.2 phycoerythrin pe, by BD (2 mentions) Cd11b fluorescein isothiocyanate, by BD (2 mentions) Ly6g pe, by BD (2 mentions) Lsrfortessa sorp, by BD (1 mentions) Igm apc, by BD (1 mentions) Facs lysing solution, by BD (1 mentions) Cyan flow cytometer, by Beckman Coulter (1 mentions) Kaluza analysis software, by Beckman Coulter (1 mentions) Cd45 pe cy7, by BD (1 mentions) Cd11b apc cy7, by BD (1 mentions) Deoxyribonuclease 1, by Merck Group (1 mentions) Mouse fc block, by BD (1 mentions) Fcs express 6, by De Novo Software (1 mentions) Cd11c pe cy5, by Thermo Fisher Scientific (1 mentions) Cd8a apc, by BD (1 mentions) Collagenase type 1, by Thermo Fisher Scientific (1 mentions) D4527, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions)

Close spelling variants of this product

CD45R/B220 (26 citations) B220-PE (26 citations) PE Rat Anti-Mouse CD45R/B220 (5 citations) CD45R-PE (3 citations) PE-Cy7 rat anti-mouse CD45R/B220 (3 citations) PE-conjugated anti-CD45R/B220 (2 citations) PE-Rat-Anti-Mouse-CD45R/B220-(RA3-6B2) (2 citations) PE anti-mouse CD45R/B220 (2 citations)

4 protocols using cd45r b220 pe

1

Spleen Immune Cell Immunophenotyping

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Following perfusion, spleens were harvested and homogenised into a single cell suspension in phosphate buffered saline (PBS) using a 100 µm Falcon cell-strainer. Red blood cells were lysed using BD FACS Lysing Solution (cat#349202) and the remaining cells blocked with FACS buffer (phosphate buffered saline with 2% fetal bovine serum) and stained using fluorochrome-conjugated antibodies against IgM (IgM-APC, cat#550676, BD Biosciences) and CD45R/B220 (CD45R/B220-PE, cat#553090, BD Biosciences). Cells were run on a BD LSRFortessa SORP (BD Biosciences) flow cytometer and the data analysed with Flow Jo Version 10 software (FlowJo, LLC/Treestar). n = 6 for each genotype and treatment. Representative results are shown in figures.
van der Hoven J., van Hummel A., Przybyla M., Asih P.R., Gajwani M., Feiten A.F., Ke Y.D., Ittner A., van Eersel J, & Ittner L.M. (2020). Contribution of endogenous antibodies to learning deficits and astrocytosis in human P301S mutant tau transgenic mice. Scientific Reports, 10, 13845.
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2

Isolation and Characterization of Murine Bone Marrow Cells

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Bone marrow was isolated from femurs and tibias of mice by flushing the marrow cavity with medium and passing the cell suspension through a cell strainer. After lysis of erythrocytes, bone marrow cells were incubated with Fc-blocking antibody (BD Pharmingen antimouse CD16/CD32, clone 2.4G2; BD Biosciences, San Jose, CA, USA), followed by staining with the following mix of antibodies: CD11b-fluorescein isothiocyanate, CD90.2-phycoerythrin (PE), CD45R/B220-PE, CD49b-PE, NK1.1-PE, Ly6G-PE, Ly6C-allophycocyanin-cyanine 7 (all BD Biosciences). Samples were acquired with a CyAn flow cytometer (Beckman Coulter Life Sciences, Indianapolis, IN, USA), and data analysis was performed with Kaluza Analysis Software (Beckman Coulter Life Sciences). The gating strategy we used is depicted in Additional file 2.
Di Ceglie I., Ascone G., Cremers N.A., Sloetjes A.W., Walgreen B., Vogl T., Roth J., Verbeek J.S., van de Loo F.A., Koenders M.I., van der Kraan P.M., Blom A.B., van den Bosch M.H, & van Lent P.L. (2018). Fcγ receptor-mediated influx of S100A8/A9-producing neutrophils as inducer of bone erosion during antigen-induced arthritis. Arthritis Research & Therapy, 20, 80.
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3

Ovarian Cell Isolation and Characterization

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Single cell suspensions of ovary cells were prepared by modifying a method previously described (Oakley et al., 2010 (link)). Ovaries were collected from WT and Esr2-PgrKO mice at 6 and 9 h after hCG injection and dissociated by 2 mL of collagenase digestion solution containing 3.5 U of collagenase type I (17100–017; Invitrogen), 1000 U of deoxyribonuclease I (D4527; Sigma), and 40 mg of BSA (017K0723; Sigma) in H-199 media (12350–039; Life Technologies, Inc.) at 37°C for 30 min. Dissociated cells (1 × 106 cells) were washed and stained according to the manufacturer’s protocol with the following antibodies alone or in varying combinations: CD16/CD32 (Mouse BD Fc Block; 553142, BD Biosciences, San Jose, CA) CD45-PE/Cy7 (552848, BD Biosciences), CD11b-APC/Cy7 (557657, BD Biosciences), CD11c-PE/Cy5 (15-0114-82, eBioscience, San Diego, CA), I-A/I-E (MHCII)-FITC (562009, BD Biosciences), Ly6G-Horizon V450 (560603, BD Biosciences), Ly6C-Brilliant Violet 450 (128033, Biolegend, San Diego, CA), CD45R/B220-PE (553089, BD Biosciences), and CD8a-APC (553035, BD Biosciences). For surface marker staining, DPBS (pH 7.4) containing heat-inactivated FBS and < 0.09% sodium azide (554656, BD Biosciences) was used as staining buffer. Stained samples were analyzed by flow cytometry (BD LSR II; BD Biosciences). Data analysis was performed using FCS Express 6 (De Novo Software, Los Angeles, CA).
Park C.J., Lin P.C., Zhou S., Barakat R., Bashir S.T., Choi J.M., Cacioppo J.A., Oakley O.R., Duffy D.M., Lydon J.P, & Ko C.J. (2020). Progesterone Receptor Serves the Ovary as a Trigger of Ovulation and a Terminator of Inflammation. Cell reports, 31(2), 107496.
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4

Quantification of Osteoclast Precursors in Murine Bone Marrow

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Numbers of osteoclast precursors in the BM were determined with flow cytometry. Bone marrow was isolated from femurs and tibias of mice by flushing the marrow cavity with medium and passing the cell suspension through a strainer. After lysis of erythrocytes, bone marrow cells were incubated with Fc-blocking antibody (BD Pharmingen antimouse CD16/CD32, clone 2.4G2; BD Biosciences, San Jose, CA, USA), followed by staining with the following mix of antibodies: CD11bfluorescein isothiocyanate (FITC), CD90.2-phycoerythrin (PE), CD45R/ B220-PE, CD49b-PE, NK1.1-PE, Ly6G-PE, Ly6C-allophycocyanin-cyanine 7 (APC-Cy7), and F4/80-PE-Cy7 (all BD Biosciences). Samples were acquired with a Gallios flow cytometer (Beckman Coulter Life Sciences, Indianapolis, IN, USA).
Ascone G., Di Ceglie I., Walgreen B., Sloetjes A.W., Lindhout E., Bot I., van de Loo F.A.J., Koenders M.I., van der Kraan P.M., Blom A.B., van den Bosch M.H.J, & van Lent P.L.E.M. (2020). High LDL levels lessen bone destruction during antigen-induced arthritis by inhibiting osteoclast formation and function. Bone, 130.
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