50 ml falcon tube
The 50-mL Falcon tube is a conical centrifuge tube with a capacity of 50 milliliters. It is commonly used in laboratory settings for various applications such as sample collection, storage, and processing.
Lab products found in correlation
3 protocols using 50 ml falcon tube
Optimizing Subsoil DNA Extraction
Pre-culture Preparation for Bacterial Growth
colony of each strain was selected, transferred with a sterile plastic
loop (VWR International), and inoculated into a 50 mL Falcon tube
(Sarstedt, Nümbrecht, Germany) with 10 mL of nutrient-rich
general media: tryptic soy broth (TSB), BD Difco, USA) for S. aureus and LB [low NaCl] (10 g/L tryptone, 5 g/L
yeast extract, and 0.5 g/L NaCl) (BD Difco, USA) for P. mirabilis. The pre-cultures were grown overnight
in a rotating incubator (New Brunswick Scientific, Innova 40/40R Incubator
Shakers, Eppendorf AG, Hamburg, Germany) at 37 °C and 200 rpm
shaking for 18 ± 1 h. After overnight incubation and prior to
starting the bacterial growth assays, the pre-cultures were washed
thoroughly to remove the rich nutrient broth and secreted metabolites.
The pre-cultures were centrifuged (3220g, 10 min,
20 °C, 5810R table centrifuge, Eppendorf AG, Hamburg, Germany)
and the cell pellets were washed twice in 20 mL of 0.9% sterile NaCl
solution (Merck Millipore, Darmstadt, Germany) and finally re-suspended
by vortexing in 10 mL of 0.9% sterile NaCl solution. The optical density
(OD) of the washed pre-cultures was measured at 620 nm [ultraviolet
(UV)/visible spectrophotometer Ultrospec 2100 pro, GE Healthcare,
Little Chalfont, UK] and the adequate inoculum volume to obtain a
starting OD of 0.1 in 200 μL was calculated.
Microbial Profiling of Used Towels
After centrifugation, the supernatant was discarded and the remaining pellet was resuspended in 700 µL of sterile 0.9% NaCl, which was used both for the determination of the bacterial count and for DNA extraction using the “Fast-DNA SPIN Kit for Soil” (MP Biomedicals GmbH, Eschwege, Germany).
For the determination of the bacterial counts, a decimal dilution series was prepared and 100 µL of each sample was spread on Tryptic Soy Agar to detect aerobic mesophilic bacteria (TSA, Merck KGaA, Darmstadt, Germany), Malt Extract Agar to detect yeasts and moulds (MEA, Merck KGaA, Darmstadt, Germany), MacConkey to detect Gram-negative bacteria (Merck KGaA, Darmstadt, Germany), Mannitol Salt Agar to detect Staphylococcus spp. (MSA, Xebios Diagnostics GmbH, Düsseldorf, Germany), and Cetrimide Agar to detect Pseudomonas spp. (Xebios Diagnostic GmbH, Düsseldorf, Germany)). Incubation was performed at 30 (MEA, MSA, and Cetrimide) or 37 °C (TSA, MacConkey).
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