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Stellux chemiluminescent rodent insulin elisa kit

Manufactured by ALPCO
Sourced in United States

The STELLUX Chemiluminescent Rodent Insulin ELISA kit is a laboratory equipment product designed for the quantitative determination of insulin levels in rodent samples using a chemiluminescent detection method.

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2 protocols using stellux chemiluminescent rodent insulin elisa kit

1

Glucose Tolerance Test Protocol

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Glucose tolerance tests (GTTs) were performed as previously described43 (link) with minor modifications. After a 6-h morning fast (ZT 0–ZT 6), basal plasma samples (t = 0) were collected at ZT 6, then mice were injected intraperitoneally with a 2 g/kg glucose bolus. Blood glucose was measured by tail bleed at set time points using a Bayer Contour Next EZ handheld glucometer (t = 15, 30, 45, 60, and 120 min). The study concluded after two hours at ZT 8. Insulin levels in plasma were measured using the STELLUX Chemiluminescent Rodent Insulin ELISA kit (ALPCO Diagnostics).
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2

Assessing Circulating Hormones in Mice

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Circulating levels of leptin and insulin were assessed by analyzing serum samples using ELISA kits: Mouse Leptin ELISA Kit (Crystal Chem, Elk Grove Village, IL, USA; 90030) and STELLUX Chemiluminescent Rodent Insulin ELISA Kit (Alpco, Salem, NH, USA; 80-INSMR-CH01). Pituitary hormone levels were measured in serum samples using the Mouse Pituitary Magnetic Bead Panel Milliplex Assay (EMD Millipore, Burlington, MA, USA; MPTMAG-49K), performed with the Luminex 200 Instrument System.
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