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Allophycocyanin cy7

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Allophycocyanin-Cy7 is a fluorescent dye conjugate used in flow cytometry applications. It is comprised of the allophycocyanin fluorescent protein coupled with the cyanine dye Cy7. The resulting conjugate has excitation and emission spectra suitable for detection on common flow cytometry instruments.

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Lab products found in correlation

Lsrfortessa x 20, by BD (1 mentions) Zombie aqua fixable viability kit, by BioLegend (1 mentions) F4 80 bm8, by BioLegend (1 mentions) Ly6g 1a8, by BioLegend (1 mentions) Mhcii m5 114, by BioLegend (1 mentions) Cd11b m1 70, by BioLegend (1 mentions) Cd11c n418, by BioLegend (1 mentions) Cd103 2e7, by BioLegend (1 mentions) Cd45 30 f11, by BioLegend (1 mentions) Facscanto 2, by BD (1 mentions) 7 aminoactinomycin d, by Thermo Fisher Scientific (1 mentions) Fortessa, by BD (1 mentions) Fluorescein isothiocyanate (fitc), by BioLegend (1 mentions) Pacific orange, by BioLegend (1 mentions)

Spelling variants of this product

CD3-allophycocyanin (APC)-CY7 (3 citations) HLA-DR-allophycocyanin-Cy7 (2 citations) Allophycocyanin/Cy7-CD45.2 (2 citations) Anti-CD3–allophycocyanin(APC)/Cy7 (2 citations) CD11b-allophycocyanin-Cy7 (2 citations) Anti-CD4 allophycocyanin (APC)/Cy7 (2 citations) IFN-γ allophycocyanin-Cy7 (2 citations)

2 protocols using allophycocyanin cy7

1

Colorful Cellular Characterization of Colonic Immunity

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Monoclonal antibodies conjugated with fluorescein isothiocynate,
phycoerythrin, phycoerythrinCy7, peridinin-chlorophyll protein complexes,
allophycocyanin, or allophycocyanin-Cy7 were purchased from BioLegend (San
Diego, CA,). Lamina propria cells in colon were stained with the antibodies
against murine CD45 (30-F11, BioLegend), CD11b (M1/70, BioLegend), CD11c
(N418, BioLegend), CD103 (2E7, BioLegend), Ly6G (1A8, BioLegend), MHC-II
(M5/114.15.2, BioLegend) and F4/80 (BM8, BioLegend) and Zombie Aqua Fixable
viability kit (423101, BioLegend). In flow cytometric analysis, at least
100,000 live samples were analyzed using BD LSRFortessa™X-20 (BD Biosciences) and FlowJo software (Tree Star, OR). The
CD45+ cells were classified into neutrophils
(CD11b+Ly6G+) and dendritic cells
(CD11c+MHC-II+CD103+).
Hayase E., Hayase T., Jamal M.A., Miyama T., Chang C.C., Ortega M.R., Ahmed S.S., Karmouch J.L., Sanchez C.A., Brown A.N., El-Himri R.K., Flores I.I., McDaniel L.K., Pham D., Halsey T., Frenk A.C., Chapa V.A., Heckel B.E., Jin Y., Tsai W.B., Prasad R., Tan L., Veillon L., Ajami N.J., Wargo J.A., Galloway-Peña J., Shelburne S., Chemaly R.F., Davey L., Glowacki R.W., Liu C., Rondon G., Alousi A.M., Molldrem J.J., Champlin R.E., Shpall E.J., Valdivia R.H., Martens E.C., Lorenzi P.L, & Jenq R.R. (2022). Mucus-degrading Bacteroides link carbapenems to aggravated graft-versushost disease. Cell, 185(20), 3705-3719.e14.
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2

Flow Cytometry Immunophenotyping Protocol

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The following antibodies or streptavidin coupled to biotin, peridinin-chlorophyll-protein–Cy5.5, fluorescein isothiocyanate, AF488, allophycocyanin, AF647, Pacific Blue, Pacific Orange, allophycocyanin-Cy7, phycoerythrin, and phycoerythrin-Cy7 were purchased from BioLegend (San Diego, Calif), eBioscience (San Diego, Calif), BD Biosciences (San Jose, Calif), or Invitrogen (Carlsbad, Calif): CD19 (clone 6D5), B220 (RA3-6B2), CD3 (145-2C11), CD4 (RM4-5), CD11c (N418), CD8α (53-6.7), CD45.1 (A20), CD45.2 (104), CD44 (IM7), CXCR5 (2G8), MHC class II (M5/114.15.2), Siglec-F (E50-2440), CD11b (M1/70), CCR3 (TG14/CCR3), Gr-1 (RB6-8C5), CD80 (16-10A1), CD86 (GL1), CD40 (3/23), OX40 ligand (RM134L), CD43 (S7), CD5 (53-7.3), IgM (R6-60.2), and IgD (11-26c). Dead cells were excluded with 7-aminoactinomycin D (eBioscience). Flow cytometry was performed on either a FACSCanto II or BD Fortessa (BD Biosciences), and data were analyzed with FlowJo software (TreeStar, Ashland, Ore).
Wypych T.P., Marzi R., Wu G.F., Lanzavecchia A, & Sallusto F. (2017). Role of B cells in TH cell responses in a mouse model of asthma. The Journal of allergy and clinical immunology, 141(4), 1395-1410.
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