The chemical composition of the bone bricks, before and after cell seeding, was analysed with the use of Energy dispersive x-ray (EDX) spectroscopy, thereby determining the concentration of calcium (Ca), carbon ©, oxygen (O) and phosphorous (P). The analysis was performed using the SEM FEG FEI Quanta 200 (FEI company, United States). The bone bricks were gold coated prior imaging. The obtained SEM images were analysed using the Oxford AZtec software (Oxford Instruments, Abingdon, Oxford, UK).
Oxford aztec software
Manufactured by Oxford Instruments
Sourced in United Kingdom
The Oxford AZtec software is a comprehensive suite of tools for the analysis and characterization of materials using electron microscopy techniques. It provides advanced data acquisition, processing, and visualization capabilities to support a wide range of applications in materials science, nanotechnology, and related fields.
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3 protocols using oxford aztec software
1
Bone Brick Composition Analysis
2
Starch Granule Morphology Analysis
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Starch granule morphology was examined using the scanning electron microscopy. The samples were transferred onto double-sided conductive carbon tape and mounted onto an aluminum SEM stubs. A thin layer of gold was used to coat the stubs to enhance conductivity. Thereafter, the sample was loaded into a Zeiss MERLIN Field Emission Scanning Electron Microscope (Carl Zeiss Microscopy, Munchen, Germany) at the Electron Microbeam Unit. A Zeiss Inlens Secondary Electron (SE) Detector, Zeiss Backscatter Electron (BSE) Detectors and Zeiss Smart SEM software were used to generate images, while the samples were chemically quantified by quantitative Energy Dispersive X-Ray Spectrometry (EDS) using an Oxford Instrument® X-Max 20 mm2 detector and Oxford Aztec software (Oxford Instruments, Oxfordshire OX13 5QX, United Kingdom). The starch samples were examined at 3 kV.
3
Bone Brick Elemental Analysis
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The chemical
composition of bone bricks before and after cell seeding was analyzed
through energy-dispersive X-ray spectroscopy (EDX), determining the
concentration of calcium (Ca), carbon (C), oxygen (O), and phosphorous
(P). The analysis was performed using the SEM FEG FEI Quanta 200 (FEI
Company). Bone bricks were gold-coated prior to imaging. The obtained
SEM images were analyzed using Oxford AZtec software (Oxford Instruments,
U.K.).
composition of bone bricks before and after cell seeding was analyzed
through energy-dispersive X-ray spectroscopy (EDX), determining the
concentration of calcium (Ca), carbon (C), oxygen (O), and phosphorous
(P). The analysis was performed using the SEM FEG FEI Quanta 200 (FEI
Company). Bone bricks were gold-coated prior to imaging. The obtained
SEM images were analyzed using Oxford AZtec software (Oxford Instruments,
U.K.).
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