SW480 and SW620 human colon cancer cell lines were obtained from American Type Culture Collection (LGC standards, Teddington, UK). Both SW cell lines were maintained at 37°C and 5% CO2 in Eagles MEM (Sigma-Aldrich, St. Louis, MO), supplemented with 10% heat inactivated fetal bovine serum (GIBCO, Invitrogen, Paisley, UK), 0.5% L-glutamine (GIBCO), and 1% penicillin/streptomycin cocktail (GIBCO). The HCT116 p53+/+ and HCT116 p53−/− isogenic human colon cancer cell lines were kindly provided by Professor Bert Vogelstein (Johns Hopkins University, Baltimore, MD). Both HCT116 cell lines were maintained at 37°C and 5% CO2 in McCoy's 5A medium (modified) (GIBCO), supplemented with 10% heat inactivated fetal bovine serum, 0.5% L-glutamine, and 1% penicillin/streptomycin cocktail.
Penicillin streptomycin cocktail
Manufactured by Thermo Fisher Scientific
Sourced in United States, Germany
Penicillin/streptomycin cocktail is a common laboratory reagent used to prevent bacterial contamination in cell culture. It is a mixture of the antibiotics penicillin and streptomycin. The core function of this product is to inhibit the growth of both gram-positive and gram-negative bacteria in cell culture media and other in vitro applications.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (17 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (6 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (5 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (4 mentions)
Bca protein assay kit, by Thermo Fisher Scientific (4 mentions)
L glutamine, by Thermo Fisher Scientific (3 mentions)
Leibovitz s l 15 medium, by Thermo Fisher Scientific (3 mentions)
Trypsin edta solution, by Thermo Fisher Scientific (3 mentions)
Mcf 7, by American Type Culture Collection (3 mentions)
Muse annexin dead cell kit, by Merck Group (3 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (2 mentions)
Sw620, by American Type Culture Collection (2 mentions)
Penicillin, by Thermo Fisher Scientific (2 mentions)
Streptomycin, by Thermo Fisher Scientific (2 mentions)
Fluorescence tagged secondary antibodies, by Thermo Fisher Scientific (2 mentions)
Cell lysis buffer 9803, by Cell Signaling Technology (2 mentions)
Protein a g agarose gel, by Santa Cruz Biotechnology (2 mentions)
Trizol, by Thermo Fisher Scientific (2 mentions)
Cd31 antibody, by BD (2 mentions)
38 protocols using penicillin streptomycin cocktail
1
Colon Cancer Cell Line Maintenance
2
SARS-CoV-2 Spike and Nucleocapsid Antibody Detection
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Vero cells were cultured in DMEM with 10% FBS (Hyclone, SH30084.03) and penicillin-streptomycin cocktail (Gibco, 15,140–122) at 37°C and 5% CO2. Anti-Spike antibody was procured from Novus Biologicals (NB100–56,578) while anti-Nucleocapsid antibody was procured from Thermo Fisher (MA5–29,982). HRP-conjugated anti-rabbit secondary antibody was purchased from Jackson ImmunoResearch (111–035–003). BPL was procured from Himedia Laboratories (TC223–100). The zinc staining kit was from G Biosciences (Reversible Zinc Stain; 786–32ZN).
3
Cell Culture Protocols for Vero and Caco2 Cells
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Vero (CCL-81) cells were cultured in Dulbecco's modified eagle medium (DMEM,
Gibco) supplemented with 10% FBS (Hyclone) and 1 × Penicillin-Streptomycin cocktail (Gibco). Caco2 cells were cultured similarly, but with 20% FBS. Cells were continuously passaged at 70–80% confluency and were maintained in a humidified cell culture incubator at 37 °C and 5% CO2.
Gibco) supplemented with 10% FBS (Hyclone) and 1 × Penicillin-Streptomycin cocktail (Gibco). Caco2 cells were cultured similarly, but with 20% FBS. Cells were continuously passaged at 70–80% confluency and were maintained in a humidified cell culture incubator at 37 °C and 5% CO2.
4
Date Seed-Based Compounds and Cancer Cell Lines
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All chemicals and enzymes used in the study were purchased from Sigma-Aldrich (St. Louis, MO, USA) unless stated otherwise. For the cell culture experiments, the various cancer cell lines MCF-7, MDA-MB-231, Caco2, PC-3 and HepG2, and mouse 3T3-L1 fibroblast, C2C12 myoblasts were obtained from the European Collection of Authenticated Cell Cultures (ECACC) (Salisbury, UK). Trypsin, rosiglitazone, 3-isobutyl-1-methylxanthine (IBMX), human recombinant insulin (4 mg/mL), and Oil Red O stain were also purchased from Sigma Aldrich (St. Louis, MO, USA). Cell viability assay kit and 2-[N-(7-Nitrobenz-2-oxa-1,3- diazol-4-yl)amino]-2-deoxy-d-glucose (2-NBDG) was obtained from Abcam (Cambridge, UK). Dulbecco's Modified Eagles Medium (DMEM) (Gibco, 12800-017), foetal bovine serum (FBS) (Gibco, 10437-028), horse serum (Gibco, 16050-122), phosphate-buffered saline (Gibco, 10010-023), penicillin-streptomycin cocktail (Gibco, 15140-148), and 0.25% Trypsin (Gibco, 25200-056) were obtained from Thermo-Fisher Scientific (Waltham, MA, USA). Date seeds from the Khalas variety were supplied by Al Foah Company, Al Ain, UAE. Date seed powder (DSP) and date seed extract (DSE) were prepared as described by Hilary et al. (14 (link)). Date seed supplemented Arabic pita bread (DSB) was baked in-house using the procedure as described by Platat et al. (32 (link)).
5
Murine lung cancer cell lines
6
Cell Culture Maintenance of Hematopoietic Cell Lines
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K562, Jurkat, and MV-4-11 cell lines were a generous gift from the Ferrell lab and confirmed to be mycoplasma negative. K562 and Jurkat were cultured in complete RPMI (cRPMI) consisting of RPMI 1640 (Gibco 22400-089) supplemented to a final concentration of 10% heat inactivated fetal bovine serum (Gibco 16140-071), 1 mM sodium pyruvate (Gibco 11360-070), and 1% penicillin/streptomycin cocktail (Gibco 15140-122). MV-4-11 were cultured in IMDM (Gibco 12440-053), supplemented to a final concentration of 10% fetal bovine serum. Cells were incubated in a water jacketed 5% CO2 incubator at 37 °C. Cells were maintained at densities between 100 thousand and 1 million cells per ml of culture media, fed every other day, and passaged every 4 days.
7
Derivation of Hematopoietic Progenitors from Mouse ESCs
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ES cell-derived HPCs were derived according to the protocol described previously by Chan et al. [3 ]. Briefly, HOXB4-transduced mouse ES cells (HM1 cell line) originally derived from the 129SvJ mouse strain were grown on feeder-seeded gelatinized flasks in ES cell culture medium with 15% fetal calf serum, 1% penicillin/streptomycin cocktail (GIBCO), 0.1 mM l -glutamine, and 1000 U/ml leukemia inhibitory factor for the maintenance of pluripotency. The ES cells were then subjected to embryoid body (EB) formation. The EBs were trypsinized and dissociated into a single cell dispersion before being replated onto ultralow-attachment Petri dishes in defined medium containing StemPro34 base media plus nutrient supplement (Life Technologies/BRL) and various hematopoietic cytokines: mIL-3 (2 ng/ml), mouse stem cell factor (100 ng/ml; R&D Systems), mIL-6 (5 ng/ml), IGF-1 (40 ng/ml; Promega), Flt3-L (10 ng/ml), and dexamethasone (1 μM; Sigma-Aldrich). The cell cultures were kept in a hypoxic incubator containing 9% CO2.
8
PPARGC1A Silencing Impacts Lung Adenocarcinoma
9
Culturing Murine Breast Cancer and Macrophage Cells
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Murine breast cancer 4T1 cells (Chinese Academy of Sciences, Shanghai, China) were cultured in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, China) supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, Invitrogen, Australia) and 1% (v/v) penicillin-streptomycin cocktail (HyClone, Logan, USA). Mouse RAW 264.7 macrophage cells (Chinese Academy of Sciences, Shanghai, China) were cultured in Dulbecco’s modified Eagle’s medium (Gibco, Thermo Fisher Scientific, China) supplemented with 10% (v/v) FBS and 1% (v/v) penicillin-streptomycin cocktail. Cells were maintained in a humidified incubator (Thermo Scientific, Thermo Fisher Scientific, China) with a constant temperature (37°C) and CO2 content (5%).
10
Isolation and Evaluation of Immune Cells
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Histopaque-1077, PBS (phosphate buffered saline), AAPH (2, 2’-azo-bis (2-amidinopropane) dihydrochloride), Trolox, and Trypan blue solution from Sigma-Aldrich; RPMI 1640, FBS (fetal bovine serum), and Penicillin-Streptomycin cocktail from Gibco; Bovine Hypothalamus Extract prepared in-house as per the method described by Maciag et al. [25 (link)]; Griess Reagent Kit for Nitrite Determination from Molecular Probes; IL-6 ELISA Kit from Novex, Life Technologies.
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