Different odorants (methyl valerate, isoamyl acetate, ethyl tiglate, and 2-heptanone; Sigma-Aldrich, USA) were used at concentrations between 0.12% and 11% of saturated vapor. A cleaned air stream was used for odorant dilution from saturated vapor. A flow dilution olfactometer 31 (link) was designed to provide a constant airflow over the nares. A vacuum-controlled odorant delivery, where the vacuum was switched off during the odorant presentation. Separate Teflon tubing lines were used for each odor to avoid cross-contamination. In a subset of experiments, the time course and relative concentration of odors were confirmed with a photo-ionization detector (PID; Aurora Scientific, Aurora, ON). During imaging trials odorants were either delivered for 2–3 s with a 60-s delay between presentations, or odors were repeatedly presented with a 6-s interstimulus interval (adaptation trials).
Ethyl tiglate
Manufactured by Merck Group
Sourced in United States
Ethyl tiglate is a chemical compound used as a laboratory reagent. It is a colorless liquid with a characteristic odor. Ethyl tiglate is commonly used in chemical synthesis and research applications.
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Lab products found in correlation
4 protocols using ethyl tiglate
1
Odorant Presentation and Delivery System
2
Odorant Presentation and Monitoring Protocol
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The odor pairs were a natural odor pair (curry powder vs. cinnamon) or a pair of pure monomolecular odorants (ethyl butyrate, valeraldehyde, isoamyl acetate, ethyl tiglate, hexanone or cineole, Sigma-Aldrich). Pure odorants were diluted 1:10 in 10 mL mineral oil and natural odorants were presented in their native state. Saturated odor vapor was further diluted with humidified clean air (1:10) by means of computer-controlled solenoid pinch valves. Odor presentation was performed using a custom-built computer interface. Odor delivery dynamics were monitored and calibrated using a mini-PID (Aurora). Odors were delivered randomly within a block (10 trials of each odorant).
3
Olfactory Stimulation Protocol
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Odor stimulation was controlled with a custom-built olfactometer25 (link). Ethyl tiglate (Sigma), a ligand for the 7250 glomerulus50 (link), was used at 0.02% to 10% of saturated vapor for all odor experiments. Odor stimuli were delivered as 2.8 to 5 sec pulses.
4
Imaging Spontaneous and Odor-Evoked Ca2+ Transients in Adult-Born Neurons
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The spontaneous Ca 2+ transients were recorded in awake mice. Prior to imaging sessions, the mice were trained for head fixation for 10-12 days, as described in (13) . Spontaneous Ca 2+ transients of adult-born JGCs were recorded at 12 dpi continuously for 2 minutes with a frame rate of 7-10 Hz. Twitch-2B was excited at 890 nm and the emitted light was split into 2 channels by a 515 nm dichroic mirror. The emission light of mCerulean3 was filtered with a 475/64 nm band-pass filter and the emission light of cpVenus CD was filtered with a 500 nm long-pass filter.
The odor-evoked responsiveness of adult-born JGCs was measured at 20 dpi. Mice were anesthetized using MMF anesthesia, the temperature was kept at ~37°C and the breathing rate was ~140 breaths per minute during the whole imaging session. Odors were applied through a custom-built flow-dilution olfactometer, positioned in front of the mouse's snout as described previously (75) . Odor mixture containing 2-hexanone, isoamyl acetate, and ethyl tiglate (purchased from Sigma-Aldrich, 0.6% of saturated vapor each) were applied as a 4-second-long pulse with an inter-pulse interval of at least 2 minutes. The odor delivery was not timed relative to respiration. Each cell was stimulated at least twice, as described in (75) .
The odor-evoked responsiveness of adult-born JGCs was measured at 20 dpi. Mice were anesthetized using MMF anesthesia, the temperature was kept at ~37°C and the breathing rate was ~140 breaths per minute during the whole imaging session. Odors were applied through a custom-built flow-dilution olfactometer, positioned in front of the mouse's snout as described previously (75) . Odor mixture containing 2-hexanone, isoamyl acetate, and ethyl tiglate (purchased from Sigma-Aldrich, 0.6% of saturated vapor each) were applied as a 4-second-long pulse with an inter-pulse interval of at least 2 minutes. The odor delivery was not timed relative to respiration. Each cell was stimulated at least twice, as described in (75) .
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