Csu x1

Manufactured by Zeiss

The CSU-X1 is a confocal scanner unit designed for high-speed, high-resolution imaging. It utilizes a Nipkow-type spinning disk to provide efficient optical sectioning and rapid data acquisition. The core function of the CSU-X1 is to enable confocal fluorescence microscopy with improved imaging speed and resolution compared to traditional laser scanning confocal systems.

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Lab products found in correlation

Axio observer z1, by Zeiss (9 mentions) Cellobserver z 1, by Yokogawa (5 mentions) Axio observer, by Zeiss (5 mentions) Tcs sp8, by Leica (4 mentions) Lsm 780, by Zeiss (4 mentions) Axiocam mrm ccd camera, by Zeiss (3 mentions) Rat tail collagen coated 35 mm glass bottomed dishes, by MatTek (3 mentions) Hc pl apo cs2 63 1.4 oil objective, by Leica (2 mentions) Axiocam mrm ccd, by Zeiss (2 mentions) Axiocam mrm, by Zeiss (2 mentions) Observer z1, by Zeiss (2 mentions) Coolsnap hq2 cooled ccd, by Hamamatsu Photonics (2 mentions) Visiview software, by Visitron (2 mentions) Lsm 710, by Zeiss (2 mentions) Lsm 880, by Zeiss (2 mentions) Zen blue software, by Zeiss (2 mentions) Fluorescence stereomicroscope, by Leica (1 mentions) Application suite x, by Leica (1 mentions) Elyra ps 1 microscope, by Zeiss (1 mentions) Dextran alexa568, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CSU-X1 A1 confocal head CSU X1 spinning disc confocal microscope CSU-X1 Spinning Disk CSU-X1 M1 confocal scanner Spinning disc CSU-X1

30 protocols using csu x1

Fluorescent Imaging of Pupae and Live Cell Ablation

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Pupae were imaged whole with a Leica Fluorescence Stereo Microscope and Leica Application Suite X (LasX, Version 3.5.2.18963).
Confocal fluorescent images were taken with a Leica TCS SP8 with an HC PL APO CS2 63×/1.4 oil objective and Leica Application Suite X (LasX, Version 3.5.2.18963). Live imaging of macrophage cultures was performed using a Zeiss CellObserver Z.1 with a Yokogawa CSU-X1 spinning disk scanning unit and an Axiocam MRm CCD camera (6.45 µm×6.45 µm) and ZenBlue 2.5 software. Ablation experiments were performed using the UV ablation system DL-355/14 from Rapp OptoElectronics, as reported previously (Lehne et al., 2022 (link)).

Hirschhäuser A., Molitor D., Salinas G., Großhans J., Rust K, & Bogdan S. (2023). Single-cell transcriptomics identifies new blood cell populations in Drosophila released at the onset of metamorphosis. Development (Cambridge, England), 150(18), dev201767.

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Multimodal Imaging of Cellular Processes

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Structure illumination microscopy images were taken with an ELYRA S.1 microscope (Cell Observer SD, 63×/1.4 oil-immersion objective). Confocal fluorescence images were taken using a Leica TCS SP8 with an HC PL APO CS2 63×/1.4 oil objective. Live imaging of macrophage cultures was performed using a Zeiss CellObserver Z.1 with a Yokogawa CSU-X1 spinning disc scanning unit and an Axiocam MRm CCD camera (6.45 µm×6.45 µm). Ablation experiments were done using a 355 nm pulsed UV laser (Rapp, Optoelectronics), as reported previously (Sander et al., 2013 (link); Rüder et al., 2018 (link)).

Hirschhäuser A., van Cann M, & Bogdan S. (2021). CK1α protects WAVE from degradation to regulate cell shape and motility in the immune response. Journal of Cell Science, 134(23), jcs258891.

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Multimodal Microscopic Imaging of Cells

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Confocal images were taken with a Leica TCS SP8 with an HC PL APO CS2 ×63/1.4 oil objective and Leica Application Suite X (LasX) software. Structure illumination microscopic images were taken with a Zeiss ELYRA PS1 Microscope with a ×63/1.4 oil objective. Live imaging of macrophages was performed using a Zeiss CellObserver Z.1 with a Yokogawa CSU-X1 spinning disk scanning unit and an Axiocam MRm CCD camera (6.45 µm × 6.45 µm) and ZenBlue 2.5 software. Laser ablation of single cells was done using the UV laser ablation system DL-355/14 from Rapp OptoElectronics. Imaging of fixed B16-F1 cells was performed with an Olympus XI-81 inverted microscope equipped with an UPlan FI ×100/1.30NA oil immersion objective or a Zeiss LSM980 confocal microscope equipped with a Plan-Neofluar ×63/1.45NA oil immersion objective using 488 nm and 561 nm laser lines. Fluorescence intensities of phalloidin-stained lamellipodia were quantified from 8-bit images captured at identical settings using ImageJ software after background subtraction. Relative mean pixel intensities in lamellipodial regions of interest are shown as whiskers-box plots including all data points. The numbers of microspikes were manually counted.

Lehne F., Pokrant T., Parbin S., Salinas G., Großhans J., Rust K., Faix J, & Bogdan S. (2022). Calcium bursts allow rapid reorganization of EFhD2/Swip-1 cross-linked actin networks in epithelial wound closure. Nature Communications, 13, 2492.

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Live Imaging of Drosophila Larval Salivary Glands

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Live imaging of larval salivary glands was performed as previously reported (Tran et al., 2015 (link)). In short, naturally secreting isolated glands were placed in a glass-bottom imaging dish, covered with a Isopore 0.1 µm PC membrane (Merck) and 50 µl Schneider's Drosophila medium. For long-term imaging, the imaging chamber was humidified with a wet tissue paper and sealed with parafilm to prevent evaporation. Dissected glands were imaged with a Zeiss CellObserver Z.1 with a Yokogawa CSU-X1 spinning disc scanning unit and an Axiocam MRm CCD camera (6.45 µm×6.45 µm). For additional Dextran imaging, dissected glands were incubated for 1 h with 200 µM Dextran-Alexa568 (molecular mass 10,000, Invitrogen) in Schneider's Drosophila medium, washed three times with medium and then placed on an imaging chamber as described above.

Lehne F, & Bogdan S. (2023). Swip-1 promotes exocytosis of glue granules in the exocrine Drosophila salivary gland. Journal of Cell Science, 136(6), jcs260366.

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Pupal Abdominal Wound Closure Dynamics

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Single epithelial cells of 18–20 h APF pupal abdomen were ablated using the UV laser ablation system DL-355/14 from Rapp OptoElectronics and wound closure was observed for 1 h every 30 s on a Zeiss CellObserver Z.1 with a Yokogawa CSU-X1 spinning disk scanning unit and an Axiocam MRm CCD camera (6.45 µm × 6.45 µm). Wound closure was analyzed by measuring the wound size in 5 min increments using freehand selections in Image J.

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Imaging Testis Development in Drosophila

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Fixed pupal testes were embedded in Fluoromount-G (SouthernBiotech) and imaged on object slides. Adult testes were imaged in live-culture dishes in PBS, to maintain their natural shape. Light micrographs were taken with a Leica M165 FC stereo microscope equipped with a Leica DFC7000 T CCD camera. All fluorescent microscopic stills were taken with a Leica TCS SP8 with a HC PL APO CS2 20x/0.75 dry objective. 4D live cell imaging was performed on developing testes of 33 h APF pupae. Prepupae were collected and timed as described elsewhere 30 (link) . Life imaging of pupal testes was performed like on egg chambers, as described before 62 (link) . Images were taken on a Zeiss Observer.Z1 with a Yokogawa CSU-X1 spinning disc scanning unit and an Axiocam MRm CCD camera (6.45 µm x 6.45 µm). Long-term imaging was performed using a LD LCI Plan-Apochromat 25x/0.8 Imm Korr DIC oil-immersion objective over 7 h, with a z-stack every 5 min. Close-ups were taken with a C Plan-Apochromat 63x/1.4 oil-immersion objective over 2 h, with a z-stack every 2 min.
Laser ablation of single cells on the testis was performed with a Rapp TB 355 laser.

Bischoff M.C., Lieb S., Renkawitz‐Pohl R., & Bogdan S. (2020). Filopodia-based contact stimulated collective migration drives tissue morphogenesis.

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Imaging and Tracking Microtubule Dynamics

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Cells were transfected with GFP-EB1ΔC-2xEGFP encoding plasmid using FuGENE 6 Transfection Reagents (Promega Corporation), according to the manufacturer’s instructions. The construct was a generous gift from Dr. T. Wittmann (University of California, San Francisco, CA) (20 (link)). The cells with physiologic levels of GFP-EB1 expression were imaged 48 hours post-transfection using Yokagawa CSU-X1 spinning disk confocal microscope under a 100× objective (Zeiss). For each cell, hundreds of GFP-EB1–labeled individual microtubule tips were imaged. Images were acquired every second for 3 minutes for a total of 180 time-lapse images per cell; approximately 10–20 representative cells per condition. Image analysis was performed using the MATLAB-based plusTipTracker algorithm, as described previously (21 (link)). The following parameters were used: search radius 5–10 pixels, max gap length 12, max shrinkage 1.0, max angle forward 25, max angle backward 10, and fluctuation radius 2. All parameters were calculated per cell; for each cell line, 10 videos containing one or two cells were acquired. Mann–Whitney test was used to calculate any statistical difference in the mean parameter values.

Galletti G., Zhang C., Gjyrezi A., Cleveland K., Zhang J., Powell S., Thakkar P.V., Betel D., Shah M.A, & Giannakakou P. (2020). Microtubule Engagement with Taxane Is Altered in Taxane-Resistant Gastric Cancer. Clinical cancer research : an official journal of the American Association for Cancer Research, 26(14), 3771-3783.

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Multimodal Microscopic Imaging of Zebrafish

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Whole-mount images were acquired with a Leica MZFL III binocular microscope coupled to an Olympus DP 71 CCD camera and Olympus cellSense software. Bright-field images were obtained with an Olympus BX51 Microscope and Olympus cellSense software. Confocal images were acquired with a Nikon A1R confocal microscope. For in vivo confocal imaging, zebrafish embryos and larvae were mounted and anaesthetized in 1% low-melting agarose (Sigma-Aldrich, A9414) containing 0.2% (w/v) tricaine on glass-bottom dishes. Z-plane images were obtained with a spinning disc confocal microscope (Zeiss, CSU-X1 Yokogawa) fitted with a 40× [1.1 numerical aperture (NA)] water immersion objective. The optical section thickness was 1 μm. Three nonconsecutive single-plane images per larvae were taken using a Zeiss LSM780 confocal microscope and a 40× (1.1 NA) water immersion objective. Confocal data were processed with ZEN 2012 software (black edition), and images were analyzed with ImageJ.

Torregrosa-Carrión R., Piñeiro-Sabarís R., Siguero-Álvarez M., Grego-Bessa J., Luna-Zurita L., Fernandes V.S., MacGrogan D., Stainier D.Y, & de la Pompa J.L. (2021). Adhesion G protein–coupled receptor Gpr126/Adgrg6 is essential for placental development. Science Advances, 7(46), eabj5445.

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Fluorescence Imaging Protocols for Cell Samples

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Images of fixed and stained samples were recorded with a confocal microscope (Zeiss LSM780, 25×/NA0.8, 40×/NA1.2/water and 63×/NA1.4). We separately recorded each color channel. For life imaging, movies with differential interference contrast (DIC) optics were recorded with a light intensity of 2.5–3.0 V, an exposure time of 80–100 ms and a frame rate of 1 image per 0.5–1 min. Fluorescent movies were recorded at an inverted spinning disc microscope (Zeiss, CSU-X1, 25×/NA0.5, 63×/NA1.3/water) with 30–50% laser intensity, 100 ms exposure time and a frame rate of 1 image per 0.5–1 min.

Liu B., Sung H.W, & Großhans J. (2019). Multiple Functions of the Essential Gene PpV in Drosophila Early Development. G3: Genes|Genomes|Genetics, 9(11), 3583-3593.

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Imaging Embryonic Development Protocols

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Fluorescent images of fixed and immunostained embryos were recorded with a confocal microscope (Zeiss LSM780, 25 × /NA0.8 and 40 × /NA1.2). For live imaging, embryos were dechorionated with hypochlorite for 90 sec, washed and lined up on a piece of apple juice agar, transferred to a coverslip coated with glue (“Tesa pack” tape dissolved in heptane), desiccated if necessary, and covered with halocarbon oil (Voltalef 10S). Time-lapse imaging of embryos was conducted on an inverted microscope with differential interference contrast optics (AxioCam camera) or with fluorescent optics and a spinning disc (Zeiss ObserverZ1, CSU-X1, AxioCam MRm, 25 × /NA0.5, 40 × /NA1.3, and 63 × /NA1.3). Images were processed with ImageJ/Fiji and Photoshop (Adobe).

Winkler F., Kriebel M., Clever M., Gröning S, & Großhans J. (2017). Essential Function of the Serine Hydroxymethyl Transferase (SHMT) Gene During Rapid Syncytial Cell Cycles in Drosophila. G3: Genes|Genomes|Genetics, 7(7), 2305-2314.

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