Fetal bovine serum (FBS) was purchased from Gibco (Gaithersburg, MD, U.S.A.). Dulbecco’s modified Eagle’s medium (DMEM), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), and dimethyl sulfoxide (DMSO) were purchased from Sigma–Aldrich (U.S.A.). The Annexin V-FITC Apoptosis Detection Kit and Hoechst Staining Kit were purchased from Solarbio (Beijing, China).
Hoechst staining kit
Manufactured by Solarbio
Sourced in China
The Hoechst Staining Kit is a laboratory reagent used for the fluorescent staining of DNA. It contains the Hoechst 33342 dye, which binds to the minor groove of DNA, allowing for the visualization and quantification of DNA content in cells.
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2 protocols using hoechst staining kit
1
Cytotoxicity Assay with FBS and MTT
2
Hoechst Staining for Apoptosis Evaluation
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A Hoechst Staining Kit was purchased from Solarbio Science & Technology Co., Ltd (Beijing, China). The cells were seeded in a 24-well plate with density of 2.5×104 cells/well. After treated with predesigned concentrations of EVO (1, 2, 4 and 8 μM for 143B cells; 0.5, 1, 2 and 4 μM for MG63 cells) or DMSO for 48h, the cells were fixed in 4% paraformaldehyde solution for 10 min, washed with PBS, stained with 200μL Hoechst 33258 per well for 30 min under dark condition and then washed twice with PBS. Finally, the stained cells were visualized at a magnification of 200× and counted from 5 randomly selected fields using a fluorescence microscope (ECLIPSE Ti, Nikon, Japan).
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