Human milk levels of TGF-β1, TGF-β2, and IgA levels were measured via commercial enzyme-linked immunosorbent assay (ELISA) kits. TGF-β1 and TGF-β2 levels were measured with a Human TGF-beta 1 DuoSet and a Human TGF-beta 2 DuoSet, respectively (R&D Systems, Minneapolis, MN, USA). IgA levels were measured with a Human IgA ELISA Quantitation Set (Bethyl Laboratories Inc., Montgomery, AL, USA). All ELISAs were conducted according to the respective manufacturers' instructions. The detection limits were 31.2 pg/mL for TGF-β1 and TGF-β2, and 7.8 ng/mL for IgA.
Human iga elisa quantitation set
Manufactured by Fortis Life Sciences
Sourced in United States
The Human IgA ELISA Quantitation Set is a laboratory product designed to quantitate human immunoglobulin A (IgA) levels in biological samples using the enzyme-linked immunosorbent assay (ELISA) technique. The set includes the necessary components to perform the IgA ELISA, such as pre-coated microplates, standards, and reagents.
Automatically generated - may contain errors
5 protocols using human iga elisa quantitation set
1
Quantification of Human Milk Immunomodulators
2
T Cell Subset Interactions Modulate Antibody Production
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Each CD4+ T cell subset (1 × 105 cells/well) was cocultured with B cells (1 × 105 cells/well) and TFH cells (5 × 104 cells/well) in round-bottomed 96-well plates. Recombinant staphylococcal enterotoxin B (SEB) (2 μg/ml; Toxin Technology, Sarasota, FL, USA) was added to stimulate B cells. Blocking antihuman IL-10 mAb (25209; R&D Systems), blocking antihuman Fas ligand mAb (100410; R&D Systems), or mouse immunoglobulin G1κ (IgG1κ) isotype control (eBioscience) was added at a concentration of 10 μg/ml on the first day of the coculture in the same experimental system as above. To assess cell-cell contact, a coculture experiment was performed using a Transwell plate (HTS Transwell-96 System [3381]; Corning Life Sciences, Acton, MA, USA) with the following cells: B cells, 3 × 105 cells/well; TFH cells, 2 × 105 cells/well; and CD4+CD25−LAG3+CD45RA− T cells, 1 × 105 cells/well. The concentrations of IgG, IgA, and IgM in the 12-day culture supernatant were measured by ELISA (Human IgG ELISA Quantitation Set, Human IgA ELISA Quantitation Set, and Human IgM ELISA Quantitation Set; Bethyl Laboratories, Montgomery, TX, USA).
3
B cell and PBMC ELISA Quantitation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
B cells and PBMCs were cultured at 3x105/well in 96 well plates. ELISA was performed using Human IgG ELISA Quantitation Set, Human IgA ELISA Quantitation Set, and Human IgM ELISA Quantitation Set (Bethyl Laboratories).
4
Infant Gut Immunity Development
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Blood and stools of the subjects at birth and at one, two, and six months of age were collected. The collected blood samples were immediately tested at a laboratory in Shiga University of Medical Science Hospital to measure the serum IgG concentrations using the nephelometry method (BM6070, JEOL Ltd., Akishima, Japan). Serum IgG levels were calculated as the ratio of serum IgG concentrations at each month (one, two, and six months) to those at birth. The collected stool samples were immediately frozen at −20 °C. Then, the stool samples were mixed with nine volumes of phosphate-buffered saline and homogenized. The homogenates were centrifuged at 20,000× g for 15 min at 4 °C and the supernatant were used for quantification of SIgA. A commercial ELISA kit (Human IgA ELISA Quantitation Set, Bethyl Laboratories, Inc., Montgomery, TX, USA) was used to measure SIgA concentrations in the stool according by the manufacture’s instruction. Stool samples that were too small to be measured (<2.5 mg) were excluded. The technicians who measured the serum IgG and stool SIgA levels were blinded to the subjects’ groups.
5
Quantification of Fecal and Breast Milk IgA
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The quantification of faecal IgA was performed as described previously [4 ]. Breast milk was centrifuged (9,100 × g, 10 min,
4°C), and the whey in it was used for the quantification of IgA using a commercial ELISA
kit (Human IgA ELISA Quantitation Set, Bethyl Laboratories, Inc., Montgomery, TX,
USA).
4°C), and the whey in it was used for the quantification of IgA using a commercial ELISA
kit (Human IgA ELISA Quantitation Set, Bethyl Laboratories, Inc., Montgomery, TX,
USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!