Penicillin g

Notably, 200 μl of BALF specimen was inoculated in 1 ml of MP liquid medium [Mycoplasma medium CM0403 (OXOID, UK); glucose and phenol red (Sigma, USA); penicillin G (Amresco, USA); and fetal bovine serum (ExCell Bio, Shanghai Ekosai Bioproducts Co., Ltd.)], mixed well and then set to incubate at 37°C in a warm box. The positive control was also set. The medium initially indicated MP growth if it changed from red to yellow and the bacterial liquid was transparent and not turbid. The specimens confirmed positive using the 200 μl of liquid medium were aspirated and inoculated in the Mp solid medium [Mycoplasma medium CM0401 and yeast extraction (OXOID, UK); glucose (Sigma, MA, USA); yeast extract (Hebei Mali Food Co., Ltd.); fetal bovine serum (ExCell Bio, Shanghai Ekosai Bioproducts Co., Ltd.); and penicillin G (Amresco, USA)]. The inoculated medium was tilted to evenly spread on the petri dish, which was placed in a 5% carbon dioxide environment culture at 37 °C. The plate was observed once daily with a low-power microscope. After about 15 days, typical “fried egg-like” colonies appeared, indicating the survival of MP culture.
The corresponding liquid positive culture was placed in an ultra-low temperature refrigerator at −80°C for numbering and storage for future use.

Verapamil (purity >98%) and oridonin (purity >98%) was obtained from Shanghai Standard Biotechnology Co., Ltd. (Shanghai, China). Acetonitrile and methanol were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Dulbecco’s modified Eagle’s medium (DMEM) and non-essential amino acid (NEAA) solution were purchased from Thermo Scientific Corp. (Logan, UT, USA). Foetal bovine serum (FBS) was obtained from GIBCO BRL (Grand Island, NY, USA). Penicillin G (10,000 U/mL) and streptomycin (10 mg/mL) were purchased from Amresco (Solon, OH, USA). Hanks’ balanced salt solution (HBSS) was purchased from GIBCO (Grand Island, NY, USA). Ultrapure water was prepared with a Milli-Q water purification system (Millipore, Billerica, MA, USA). All other chemicals were of analytical grade or better.

Sorafenib (purity >98%), β-nicotinamide adenine dinucleotide phosphate (NADP) and Lucifer yellow was purchased from Sigma (St. Louis, MO). Berberine (purity >98%) and triptolide (purity >98%) were purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Rat liver microsomes were purchased from BD GentestTM (Becton Dickinson, Franklin Lakes, NJ). The Alkaline Phosphatase Assay Kit was provided by Nanjing Jiancheng Bioengineering Institute (Nanjing, China). Acetonitrile and methanol were purchased from Fisher Scientific (Fair Lawn, NJ). Formic acid was purchased from Anaqua Chemicals Supply Inc. Limited (Houston, TX). Ultrapure water was prepared with a Milli-Q water purification system (Millipore, Billerica, MA). All other chemicals were of analytical grade or better.
Dulbecco’s modified Eagle’s medium (DMEM) and non-essential amino acid (NEAA) solution were purchased from Thermo Scientific Corp. (Logan, UT). Foetal bovine serum (FBS) was obtained from GIBCO BRL (Grand Island, NY). Penicillin G (10,000 U/mL) and Streptomycin (10 mg/mL) were purchased from Amresco (Solon, OH). Hanks' balanced salt solution (HBSS) was purchased from GIBCO (Grand Island, NY).