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Dry acetonitrile

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Dry acetonitrile is a high-purity solvent used in various laboratory applications. It is a colorless, volatile liquid with a characteristic odor. Dry acetonitrile is commonly utilized as a mobile phase component in chromatographic techniques, such as high-performance liquid chromatography (HPLC) and gas chromatography (GC), as well as in other analytical procedures requiring a reliable, high-purity solvent.

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Lab products found in correlation

Tmt 10 plex reagent, by Thermo Fisher Scientific (2 mentions) Reagent 126, by Thermo Fisher Scientific (2 mentions) Hydroxylamine, by Merck Group (2 mentions) Sodium diethyldithiocarbamate trihydrate, by Merck Group (1 mentions) Ammonium perrhenate, by Merck Group (1 mentions) Tetraethylthiuram disulfide, by Merck Group (1 mentions) Ammonium sulfide, by Merck Group (1 mentions) Tetraethylammonium bromide, by Merck Group (1 mentions) Chromium 3 chloride hexahydrate, by Merck Group (1 mentions) Manganese 2 acetate tetrahydrate, by Merck Group (1 mentions) Molybdenum hexacarbonyl mo co 6, by Merck Group (1 mentions) Acetone, by Merck Group (1 mentions) Pentane, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Hexane, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Hydrochloric acid (hcl), by Merck Group (1 mentions) Dichloromethane, by Merck Group (1 mentions) Isopropanol, by Merck Group (1 mentions) Diethyl ether, by Merck Group (1 mentions)

6 protocols using dry acetonitrile

1

Peptide Labeling with 10-plex TMT Reagents

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Peptides were labeled with 10-plex TMT reagents (Thermo) as previously described62 (link). Briefly, TMT reagents were reconstituted in dry acetonitrile (Sigma) at 20 μg/mL. Dried peptides were re-suspended in 30% dry acetonitrile in 200 mM HEPES, pH 8.5, and 8 μL of the appropriate TMT reagent was added to the peptides. Reagent 126 (Thermo) was used as a bridge between mass spectrometry runs. Remaining reagents were used to label samples in a random order. Labeling was carried out for 1 hour at room temperature and was quenched by adding 9 μL of 5% hydroxylamine (Sigma) which was allowed to react for 15 mins at room temperature. Labeled samples were acidified by adding 50 μL of 1%TFA, pooled into appropriate 10-plex TMT samples and desalted with C18 Sep-Paks.
Grainger S., Nguyen N., Richter J., Setayesh J., Lonquich B., Oon C.H., Wozniak J.M., Barahona R., Kamei C.N., Houston J., Carrillo-Terrazas M., Drummond I.A., Gonzalez D., Willert K, & Traver D. (2019). EGFR is required for Wnt9a/Fzd9b signalling specificity in haematopoietic stem cells. Nature cell biology, 21(6), 721-730.
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2

Peptide Labeling with 10-plex TMT Reagents

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Peptides were labeled with 10-plex TMT reagents (Thermo) as previously described62 (link). Briefly, TMT reagents were reconstituted in dry acetonitrile (Sigma) at 20 μg/mL. Dried peptides were re-suspended in 30% dry acetonitrile in 200 mM HEPES, pH 8.5, and 8 μL of the appropriate TMT reagent was added to the peptides. Reagent 126 (Thermo) was used as a bridge between mass spectrometry runs. Remaining reagents were used to label samples in a random order. Labeling was carried out for 1 hour at room temperature and was quenched by adding 9 μL of 5% hydroxylamine (Sigma) which was allowed to react for 15 mins at room temperature. Labeled samples were acidified by adding 50 μL of 1%TFA, pooled into appropriate 10-plex TMT samples and desalted with C18 Sep-Paks.
Grainger S., Nguyen N., Richter J., Setayesh J., Lonquich B., Oon C.H., Wozniak J.M., Barahona R., Kamei C.N., Houston J., Carrillo-Terrazas M., Drummond I.A., Gonzalez D., Willert K, & Traver D. (2019). EGFR is required for Wnt9a/Fzd9b signalling specificity in haematopoietic stem cells. Nature cell biology, 21(6), 721-730.
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3

Synthesis of Transition Metal Complexes

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Molybdenum hexacarbonyl Mo(CO)6 (98.0%), tetraethylthiuram disulfide (≥97%), ammonium tetrathiotungstate (≥99.99%), sodium diethyldithiocarbamate trihydrate (≥98%), sulfur (≥99.98%), ammonium sulfide (20 wt% in water), tetraethylammonium bromide (≥99%), ammonium perrhenate (≥99.0%), bis(diethylthiocarbamoyl)disulfide (≥97%), manganese(II) acetate tetrahydrate (≥99%), chromium(III) chloride hexahydrate (≥96%), and silica gel (40–63 µm particle size) were purchased from Sigma–Aldrich and used without further purification. Acetone (≥99.0%) dichloromethane (≥99.0%), methanol (≥99.5%), diethyl ether (≥99.0%), acetonitrile (≥99.9%), hexane (≥97.0%), dry acetonitrile (≥99.80%), pentane (≥99.0%), isopropanol (≥99.5%), and hydrochloric acid (37%) were purchased from Sigma–Aldrich and used as received.
Qu J., Elgendy A., Cai R., Buckingham M.A., Papaderakis A.A., de Latour H., Hazeldine K., Whitehead G.F., Alam F., Smith C.T., Binks D.J., Walton A., Skelton J.M., Dryfe R.A., Haigh S.J, & Lewis D.J. (2023). A Low‐Temperature Synthetic Route Toward a High‐Entropy 2D Hexernary Transition Metal Dichalcogenide for Hydrogen Evolution Electrocatalysis. Advanced Science, 10(14), 2204488.
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4

Synthesis and Purification of Glucuronic Acid Glycosides

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Synthesis of the glucuronic acid donor was carried out according to Jongkees and Withers (2011) in a dark environment [18 (link)]. To IXN, in a dry flask in argon atmosphere, 20 mg of IXN was dissolved in dry acetonitrile (dried with molecular sieves, Sigma Aldrich, Saint Louis, MO, USA) followed by the addition of 27 mg (1,2 eq) of acetobromo-α-d-glucuronic acid methyl ester. The reaction was left to stir for 5 min before AgO2 (2,5 eq, 32.4 mg) was added. The reaction was left at room temperature for 20 h before being filtered through a celite plug, followed by silica gel (mesh 80–200), filtered through filter paper, and concentrated. The reaction product was resuspended in EtOAc and washed with H2CO3 twice, with water twice, and with brine once before being concentrated. Deprotection was conducted by the addition of MeOH/H2O, changing the pH to 12, and leaving the product to stir for a further 3 h, before performing liquid–liquid extraction. Lastly, the obtained suspension was filtered through celite, and the filtrate was columned with Sephadex LH-20 and prepared with preparative HPLC according to previous work [43 (link)]. To obtain 6-PN-7-O-Glc and 8-PN-7-O-Glc, a mixture of 6-PN and 8-PN was used as starting material.
Buckett L., Schönberger S., Spindler V., Sus N., Schoergenhofer C., Frank J., Frank O, & Rychlik M. (2022). Synthesis of Human Phase I and Phase II Metabolites of Hop (Humulus lupulus) Prenylated Flavonoids. Metabolites, 12(4), 345.
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5

Dye-Sensitized Solar Cell Fabrication

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Unless otherwise stated,
all materials were
purchased from commercial suppliers and used without further purification;
potassium ferrocyanide (K4Fe(CN)6), potassium
ferricyanide (K3Fe(CN)6), lithium iodide, iodine,
lithium bis(trifluoromethanesulfonyl)imide, tetrabutylammonium
hexafluorophosphate, and dry acetonitrile from Sigma-Aldrich;
conductive glass substrates (TEC 15) from Pilkington; 1,2-dimethyl-3-propylimidazolium
iodide and N-methyl benzimidazole from TCI; Cu(tmby)2TFSI1;2 and Co(bpy)3(PF6)2;3 complexes as well as the Y123 dye from Dyenamo (Stockholm).
Michaels H, & Freitag M. (2022). Assessment of TiO2 Blocking Layers for CuII/I-Electrolyte Dye-Sensitized Solar Cells by Electrochemical Impedance Spectroscopy. ACS Applied Energy Materials, 5(2), 1933-1941.
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6

Synthesis of Metal Complexes for Photocatalysis

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Dry acetonitrile was purchased from Sigma-Aldrich and used as received. All other reagents were purchased from Sigma-Aldrich without further purification. N,N,N′,N′-((CH2)3PO3H2)4,4′-dianiline (DA) was prepared according to the reported literature.55 (link) The [Ru(4,4′-(PO3H2CH2)2-2,2′-bipyridine)2(2,2′-bipyridine)2]2+ (RuCP22+) chromophore was made according to previously published procedures.56 (link) The Re complex was prepared following the protocol reported by Schreier et al.20 (link)
Wang D., Wang Y., Brady M.D., Sheridan M.V., Sherman B.D., Farnum B.H., Liu Y., Marquard S.L., Meyer G.J., Dares C.J, & Meyer T.J. (2019). A donor-chromophore-catalyst assembly for solar CO2 reduction †Electronic supplementary information (ESI) available. See DOI: 10.1039/c8sc03316a. Chemical Science, 10(16), 4436-4444.
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