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Anti ccr3

Manufactured by BD
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Anti-CCR3 is a laboratory instrument used for the detection and quantification of the CCR3 receptor, which is expressed on the surface of certain cell types. This product provides researchers with a tool to study the role of the CCR3 receptor in various biological processes and disease states.

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Lab products found in correlation

Anti siglecf, by BD (3 mentions) Anti cd4, by BD (3 mentions) Anti cd45, by Thermo Fisher Scientific (2 mentions) Kaluza, by Beckman Coulter (2 mentions) Anti gr 1, by BD (2 mentions) Anti cd11c, by BD (2 mentions) Anti b220, by BD (2 mentions) Anti cd23, by BD (2 mentions) Cellquest software, by BD (2 mentions) Anti cd3, by BD (2 mentions) Anti cd8, by BD (2 mentions) Anti cd69, by BD (2 mentions) Anti cd11b, by BD (2 mentions) Facscanto, by BD (1 mentions) Moflo xdp, by Beckman Coulter (1 mentions) Phospho erk1 2, by Cell Signaling Technology (1 mentions) Igg2b, by Thermo Fisher Scientific (1 mentions) Anti cd11b, by R&D Systems (1 mentions) Facscalibur, by BD (1 mentions) 123count beads, by Thermo Fisher Scientific (1 mentions)

5 protocols using anti ccr3

1

Phospho-ERK1/2 Activation in Eosinophils

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Total bone marrow cells were stained with anti-CD45 (ebioscience), anti–Siglec-F (BD Bioscience) and anti-CCR3 (BD Bioscience). The mature eosinophils (triple positive) were sort by MoFlo XDP (Beckman Coulter). The cells were activated with bronchoalvelolar lavage fluid (BALF) which was obtained from the lungs of CC10-Il13Tg/Pirb+/+ mice for the indicated time points (0, 5 and 10 minutes), and cells were fixed in 4% paraformaldehyde/PBS and permeabilized using saponin-based permeabilization buffer (X1, invitrogen). Cells were stained with phospho-ERK1/2 (Cell signaling). Events were acquired by FACSCanto (BD Bioscience), and data were analyzed using the Kaluza (BeckmanCoulter) or FlowJo (TreeStar) software. For phosphoflow analysis, the mean fluorescence intensity (MFI) for each time point, in each biological repeat, was normalized to baseline and expressed as the fold change over baseline.
Ben Baruch-Morgenstern N., Mingler M.K., Stucke E., Besse J.A., Wen T., Reichman H., Munitz A, & Rothenberg M.E. (2016). Paired immunoglobulin-like receptor B inhibits IL-13–driven eosinophil accumulation and activation in the esophagus. Journal of immunology (Baltimore, Md. : 1950), 197(3), 707-714.
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2

Multiparameter Flow Cytometry of Immune Cells

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Flow cytometric analysis of bone marrow, peripheral blood cells or enzymatically digested esophagus was conducted using the following antibodies: anti-CD11b (R&D), anti–GR-1 (BD Bioscience), anti–Siglec-F (BD Bioscience), anti-CCR3 (BD Bioscience), anti–PIR-A/B (ebioscience), IgG2b (ebioscience), anti-CD45 (ebioscience) and anti-CD11c (BD Bioscience). Cell counts were conducted using 123count beads (ebioscience) according to the manufacturers’ instructions. In all experiments, at least 50,000 events were acquired by (FACSCalibur, BD Bioscience), and data were analyzed using the Kaluza (BeckmanCoulter) or FlowJo (TreeStar) softwares.
Ben Baruch-Morgenstern N., Mingler M.K., Stucke E., Besse J.A., Wen T., Reichman H., Munitz A, & Rothenberg M.E. (2016). Paired immunoglobulin-like receptor B inhibits IL-13–driven eosinophil accumulation and activation in the esophagus. Journal of immunology (Baltimore, Md. : 1950), 197(3), 707-714.
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3

Flow Cytometric Analysis of Murine Immune Cells

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The minced dorsal skin of mice was incubated in PBS containing 1 mg/mL collagenase IV and 2 mg/mL dispase for 40 min at 37 °C. Later, the cells were stained with anti-CD3, anti-CD4, anti-CD8, anti-CD19, anti-CD23, anti-CD69, anti-B220, anti-CCR3, and anti-CD11b antibodies (BD Biosciences Pharmingen, San Diego, CA, USA) in staining buffer (PBS containing 1% v/v fetal bovine serum and 0.01% w/v sodium azide) for 30 min on ice. The cells were then analyzed using a fluorescence-activated cell sorting analyzer with Cell-Quest software (BD Biosciences).
Lee Y.S., Yang W.K., Jo E.H., Ho Shin S., Lee Y.C., Park M.C, & Kim S.H. (2020). NCM 1921, a Mixture of Several Ingredients, Including Fatty Acids and Choline, Attenuates Atopic Dermatitis in 1-Chloro-2,4-Dinitrobenzene-Treated NC/Nga Mice. Nutrients, 12(1), 165.
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4

Flow Cytometric Analysis of Lung Cells

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Cells from lung tissue and BALF were stained with the indicated antibodies in staining solution (PBS containing 1% FBS and 0.01% NaN3) for 10 min on ice. The stained cells analyzed by two-color flow cytometry on an FACSCalibur using CellQuest software (BD Biosciences, Mountain View, Calif, USA). All antibodies such as anti-CD3, anti-CD4, anti-CD8, anti-B220, anti-CD23, anti-CD69, anti-CD11b, anti-Gr-1, and anti-CCR3 for flow cytometric analysis were purchased from BD PharMingen (San Diego, Calif, USA).
Sung Y.Y., Kim S.H., Kim D.S., Lee J.E, & Kim H.K. (2017). Illicium verum Extract and Trans-Anethole Attenuate Ovalbumin-Induced Airway Inflammation via Enhancement of Foxp3+ Regulatory T Cells and Inhibition of Th2 Cytokines in Mice. Mediators of Inflammation, 2017, 7506808.
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5

Multiparameter Flow Cytometry Analysis

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We purchased fluorescence-labeled anti-CCR4 (clone 2G12), anti-CCR3 (clone J073E5), anti-CD11c (clone N418), antieIL-4 (clone 11B11), antieIL-17A (clone TC11-18H10.1), anti-CD45 (clone 30-F11), and anti-CD4 (clone GK1.5) from Biolegend, and anti-siglec-F (clone E50-2440) from BD Biosciences (San Diego, CA). Cells were incubated for 30 minutes with a mixture of anti-CD45 and anti-CD11c, anti-CD4, anti-CCR4, anti-CCR3, or anti-siglec-F. For intracellular staining, cells were then fixed and permeabilized (Cytofix/Cytoperm kit; BD Biosciences) and subsequently stained intracellularly with antieIL-4 and antieIL-17A. After washing, cells were immediately analyzed on a FACSForttessa (BD Biosciences) and analyzed with FlowJo software (Tree Star Inc, Ashland, OR). For the staining of IL-4 and IL-17A, cells were stimulated with phorbol 12-myristate 13-acetate and ionomycin for 4 hours.
Matsuo K., Nagakubo D., Komori Y., Fujisato S., Takeda N., Kitamatsu M., Nishiwaki K., Quan Y.S., Kamiyama F., Oiso N., Kawada A., Yoshie O, & Nakayama T. (2018). CCR4 Is Critically Involved in Skin Allergic Inflammation of BALB/c Mice. The Journal of investigative dermatology, 138(8).
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