Recombinant mouse thrombopoietin tpo

To assess their multilineage differentiation capacity, total EML cells and purified CD34^neg, CD34^low, CD34^int and CD34^high EML cell subsets were plated in 2 ml of 0.3% low melting temperature agarose (SeaPlaque, Cambrex) with 2X IMDM (Gibco), 20% heat inactivated equine serum (Hyclone) and 10% SCF-conditioned medium in replicate 6-well plates (10³ cells/well)37 (link)38 (link). The media was supplemented with: 0.1 ng/ml of recombinant mouse interleukin 3 (IL-3) (R&D Systems) for generation of granulocyte-macrophage colony forming units (CFU-GM); 8 U/ml of recombinant human erythropoietin (Epo) (Ortho Biotech) for generation of burst-forming erythroid units (BFU-E); and 3 ng/ml of recombinant mouse Thrombopoietin (Tpo) (R&D Systems) for generation of megakaryocyte colony forming units (CFU-Meg). Cultures were maintained at 37°C, 5% CO₂ for 7–10 days. The BFU-E, CFU-GM and CFU-Meg colonies were counted 7–10 days after plating. Data from multiple independent experiments (n = 15) are shown as mean ± standard deviation (SD).