The following reagent grade or greater chemicals were used: concentrated hydrochloric acid, ferric chloride hexahydrate, trolox, and 2,4,6‐tris(2‐pyridyl)‐s‐triazine (TPTZ) from Sigma Aldrich; ACS certified grade ferrous sulfate heptahydrate, glacial acetic acid, molecular biology grade ethanol (200 proof), and DPPH from Fisher Scientific; sodium acetate trihydrate (≥99%) from Avantor; gallic acid monohydrate from Acros Organics; Folin‐Ciocalteu reagent from Merck; sodium carbonate monohydrate from J. T. Baker Chemical; and ultrapure water produced on‐site. Nitrogen, 99.998% and helium, 99.999% were from Praxair. Chemicals were used without further purification. All chemicals originated from the United States.
Gallic acid monohydrate
Manufactured by Thermo Fisher Scientific
Sourced in Belgium
Gallic acid monohydrate is a chemical compound commonly used as a reagent in analytical and research applications. It is a crystalline solid with the chemical formula C6H2(OH)3COOH·H2O. The monohydrate form of gallic acid is a widely available and versatile laboratory product.
Automatically generated - may contain errors
Lab products found in correlation
Folin ciocalteu reagent, by Merck Group (3 mentions)
Sodium acetate trihydrate, by Avantor (2 mentions)
Molecular biology grade ethanol, by Thermo Fisher Scientific (2 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Thermo Fisher Scientific (2 mentions)
Ferric chloride hexahydrate, by Merck Group (2 mentions)
Glacial acetic acid, by Thermo Fisher Scientific (2 mentions)
Trolox, by Merck Group (1 mentions)
Concentrated hydrochloric acid, by Merck Group (1 mentions)
Helium, by Linde (1 mentions)
Hplc grade ethanol, by Merck Group (1 mentions)
2 4 6 tris 2 pyridyl s triazine tptz, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Ultra pure water, by Cayman Chemical (1 mentions)
Glycerol, by Thermo Fisher Scientific (1 mentions)
Trolox, by Thermo Fisher Scientific (1 mentions)
Folin ciocalteu s reagent, by ITW Reagents (1 mentions)
Sodium acetate anhydrous, by ITW Reagents (1 mentions)
D glucose anhydrous, by Thermo Fisher Scientific (1 mentions)
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts), by ITW Reagents (1 mentions)
L ascorbic acid, by Thermo Fisher Scientific (1 mentions)
6 protocols using gallic acid monohydrate
1
Comprehensive Antioxidant Capacity Assay
2
Antioxidant Capacity Measurement Protocols
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The following reagent grade or greater chemicals were used for the FRAP assay and the TPC assay: concentrated hydrochloric acid, ferric chloride hexahydrate, HPLC grade ethanol, and 2,4,6‐Tris(2‐pyridyl)‐s‐triazine (TPTZ) from Sigma Aldrich; ACS certified grade Ferrous sulfate heptahydrate and glacial acetic acid from Fisher Scientific; Sodium acetate trihydrate (≥99%) from Avantor; Gallic acid monohydrate from Acros Organics; Folin‐Ciocalteu reagent from Merck; Sodium carbonate monohydrate from J. T. Baker Chemical and Ultrapure water from Cayman Chemical Company. Ethanol that was used for the extraction was 95% pure and denatured with 5% wood spirit. Nitrogen (99.998%) and helium (99.999%) were from Praxair. Chemicals were used without further purification. All chemicals originated from the United States.
3
Reagents and Materials Used in Antioxidant Analysis
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D-glucose anhydrous (MW = 180.16 g mol−1), glycerol (MW = 92.09 g mol−1), and acetic acid glacial (MW = 60.052 g mol−1) were purchased from Fisher Scientific (Hampton, NH, USA). ABTS (MW = 548.69 g mol−1), potassium peroxodisulphate (MW = 270.32 g mol−1), sodium acetate anhydrous (MW = 82.03 g mol−1), and Folin–Ciocalteu's reagent (MW = 260.20 g mol−1) were purchased from PanReac (Barcelona, Spain). Trolox (MW = 250.29 g mol−1) was purchased from ThermoScientific (Waltham, Massachusetts, USA). Gallic acid monohydrate (MW = 188.12 g mol−1) was purchased from AcrosOrganics (Geel, Belgium). Sodium carbonate anhydrous (MW = 105.99 g mol−1) was purchased from LabChem (Zelienople, Pennsylvania, US).
4
Antioxidant and Enzyme Inhibition Assays
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Enzymes (i.e., α-glucosidase, α-amylase Type IV-B, and lipase Type II sourced from porcine pancreas), p-nitrophenyl-α-D-glucopyranoside (p-NPG), acarbose, and 4-methylumbelliferyl oleate (4MUO) were purchased from Sigma-Aldrich. DPPH was obtained from Thermo Fisher Scientific, and 2,4,6-tri(2-pyridyl)-s-triazine, gallic acid monohydrate, ethylenediaminetetraacetic acid (EDTA), 3-(2-pyridyl)-5,6-diphenyl-1,2,4-triazine-p,p-disulfonic acid monosodium salt hydrate (Ferrozine), L-ascorbic acid, and Folin-Ciocalteu reagent were purchased from Acros Organics. All chemicals employed were of analytical quality. Ten kilogram each of fresh GLVs, including spring onions (Allium cepa), bunching onions (Allium fistulosum), and celery (Apium graveolens), along with hen eggs, sucrose, soybean oil, wheat flour, sodium chloride, and baking powder (i.e., sodium bicarbonate), were obtained from the Hua Takhe market (Bangkok) then taken directly to King Mongkut’s Institute of Technology Ladkrabang. Before use, the aerial part of all GLVs was carefully rinsed with tap water to remove dirt and then dried on screens.
5
Synthesis and Characterization of Phenolic Compounds
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Gallic acid monohydrate (ACS), 3,4,5‐trimethoxyphenol (>98.5%), ascorbic acid (99%) and sodium nitrate (extra pure) were purchased from Acros Organics; 3,4‐dihydroxybenzoic acid (97%) from Alfa Aesar; acetonitrile (>99.9%), ethyl acetate (>99.8%), diethyl ether (>99.5%) and methanol (>99.8%) from Fisher Chemical; sodium hydroxide (>98%) from José Manuel Gomes dos Santos; anhydrous sodium sulphate (99%), boron tribromide solution in anhydrous dichloromethane and anhydrous dichloromethane (99.7%) from Thermo Scientific; agar and resorcinol (99%) from Panreac; all other chemicals of the highest grade from Sigma‐Aldrich/Merck.
6
Cell Culture and Cytotoxicity Assay
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The following reagent grade or greater chemicals were utilized: HyClone™ McCoy's 5A media (1.5mM L‐Glutamine, 2.2g/L sodium bicarbonate), Dulbecco's Modified Eagle's Medium‐high glucose (4.5g/L Glucose, L‐Glutamine, and Sodium Pyruvate) from VWR Life Sciences; Fetal Bovine Serum from Omega Scientific, Gibco™ Trypsin‐EDTA (0.25%), phenol red, Hyclone™ Phosphate‐Buffered Saline ((1X) 0.0067M PO4) without calcium, magnesium, phenol red, HyClone™ Trypan Blue Solution from Thermo Fisher Scientific, Optima LC/MS grade methanol and water, Optima LC/MS grade formic acid, molecular biology grade ethanol (200 proof) from Fisher Scientific; alamar Blue® from VWR, Gallic Acid monohydrate from Acros Organics; Folin–Ciocalteu reagent from Merck; Sodium Carbonate–Monohydrate from J. T. Baker Chemical; and ultrapure water produced on‐site. Nitrogen (99.998% purity) and carbon dioxide (99.5% purity) were from Praxair. Chemicals were used without further purification.
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