Cd105 percp vio700

Immunophenotype analysis was performed on three different representative populations for each cell type. For ASCs, the experiments were conducted at passage 3 and cells incubated for 10 min at 4 °C in the dark with anti-human antibodies: CD90-FITC, CD44-PE CD73-PE, CD105-PerCP-Vio700, CD34-PE-Vio770, and CD45-PE-Vio770 (Miltenyi Biotec, Bergisch Gladbach, Germany). For FLSs, analyses were conducted at passages 0 and 1, and cells stained for 10 min at 4 °C in the dark with anti-human antibodies: CD73-PE (Miltenyi) and CD14-FITC (Ancell Corporation, Bayport, MN, USA). Unstained samples for each population were used as negative controls, and data were acquired by the CytoFLEX flow cytometer (Beckman Coulter, CA, USA) collecting a minimum of 50,000 events.

hMSCs were incubated for 30 min at 4 °C in the dark with anti-human antibodies: CD90-FITC, CD73-PE, CD105-PerCP-Vio700, CD45-PE-Vio770 (Miltenyi Biotec, Bergisch Gladbach, Germany). Unstained samples were used as negative controls, and data were acquired with a CytoFLEX flow cytometer (Beckman Coulter, CA, USA) collecting a minimum of 30,000 events.