Orion 3 star

Manufactured by Thermo Fisher Scientific

Sourced in United States, Singapore

The Orion 3 Star is a laboratory instrument designed for pH, ion, and conductivity measurement. It provides precise and reliable results for various analytical applications.

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Orion 3 Star pH meter (19 citations) Orion™ 3-Star Benchtop pH Meter (19 citations) Orion 3-star pH Benchtop (12 citations) Orion 3 Star Plus pH meter (4 citations) Orion 3 star benchtop (4 citations) Orion 3-star meter (3 citations) Orion 3-Star Dissolved Oxygen meter (2 citations) Orion 3-Star Dissolved Oxygen meter and probe (2 citations) Thermo Orion 3 Star pH Benchtop Meter (2 citations) Thermo Scientific Orion 3-Star equipment (2 citations)

61 protocols using orion 3 star

Quantifying Hypersensitive Response and Electrolyte Leakage in Arabidopsis

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For both HR and electrolyte leakage, Arabidopsis Col-0 and GAPDH KO leaves were infiltrated using a needleless syringe with 4×10⁷ CFU/ml of Pst DC3000 and Pst DC3000 (AvrRpt2). After infiltration, plants were placed under a light bank (100 μE/m²/s) and HR was scored at 10 h post inoculation. For electrolyte leakage, two leaves per plant were infiltrated across four biological replicates per genotype. Total tissue was harvested using a cork borer to generate 1.5 cm² of leaf discs (six total leaf discs). Leaf discs were placed in distilled water (20mL in a 50mL conical tube) for 1h and individual biological replicates kept separate. Leaf discs were transferred to a 12-well tissue culture plate (Corning) containing 4mL of distilled water per well and placed under the light bank. Conductivity was measured using the Orion 3 Star conductivity meter (Thermo Scientific). All experiments were repeated at least three times.

Henry E., Fung N., Liu J., Drakakaki G, & Coaker G. (2015). Beyond Glycolysis: GAPDHs Are Multi-functional Enzymes Involved in Regulation of ROS, Autophagy, and Plant Immune Responses. PLoS Genetics, 11(4), e1005199.

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Surface pH Measurement of Films

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To measure the surface pH of films, they were kept in a Petri dish containing 10 mL of distilled water for 10 min to swell. After swelling, the surface pH was measured by using an Orion 3 Star pH-meter glass electrode (Thermo Scientific, Waltham, MA, USA). pH probe was in contact with the surface of each film and was allowed to equilibrate for 1 min.

Szekalska M., Wróblewska M., Trofimiuk M., Basa A, & Winnicka K. (2019). Alginate Oligosaccharides Affect Mechanical Properties and Antifungal Activity of Alginate Buccal Films with Posaconazole. Marine Drugs, 17(12), 692.

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Alkalinity Measurement Protocol

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Alkalinity was evaluated by measuring pH according to a previously published study.¹¹ In brief, we prepared specimen (1-mm thickness and 5-mm diameter) and allowed to set completely. After setting, we inserted one tablet into 10 mL of deionized water. Then, the pH value was measured using a pH meter (Orion 3 Star; Thermo Fisher Scientific, Singapore).

Choi Y., Bae J.L., Kim H.J., Yu M.K., Lee K.W, & Min K.S. (2019). Effects of dodecacalcium hepta-aluminate content on the setting time, compressive strength, alkalinity, and cytocompatibility of tricalcium silicate cement. Journal of Applied Oral Science, 27, e20180247.

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pH Measurement of Dental Materials

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The pH was measured according to the criteria used in a previously published study [10 (link)]. Specimens (1-mm thickness and 5-mm diameter) of the tested materials were prepared and allowed to set for 1 day (n = 3). After setting, one tablet was added to 10 mL of deionized water. Then, the pH value was measured using a pH meter (Orion 3 Star; Thermo Scientific, Singapore). The apparatus was previously calibrated with pH 7.0 and 4.0 solutions.

Lim E.S., Park Y.B., Kwon Y.S., Shon W.J., Lee K.W, & Min K.S. (2015). Physical properties and biocompatibility of an injectable calcium-silicate-based root canal sealer: in vitro and in vivo study. BMC Oral Health, 15, 129.

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Nanoparticle Characterization Protocol

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The chemical and morphological characterizations for the nanoparticles were realized by Shimadzu UV-1800 (UV–VIS), PerkinElmer Frontier model FT-IR, Bruker D8 Advance model X-ray diffraction (XRD) with a Cu K_α radiation source in 2θ range from 10° to 90°, TEM-120 kV transmission electron microscope (TEM) and Carl Zeiss EVO-LS 10 scanning electron microscope (SEM). Common drift method [30 (link)] was used to determine the pH (pHpzc) of nanoparticles and nanocomposites at the zero charge point. For this, 50 mL of 0.01 M NaCl solution was placed in a closed flask. The pH value was adjusted to a value between 2.0 and 12.0 by adding 0.1 M HCl and/or 0.1 M NaOH solutions. Then, 0.05 g of each nanoparticles/nanocomposites was added, and the final pH was measured using the pH meter (Thermo Scientific, Orion 3 Star) after 24 h under shaking at room temperature. The intersection point of initial pH and final pH values was determined to be pHpzc.

Şahin M., Arslan Y, & Tomul F. (2022). Removal of naproxen and diclofenac using magnetic nanoparticles/nanocomposites. Research on Chemical Intermediates, 48(12), 5209-5226.

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Evaluating Collagen Peptide's Impact on Acid Production by S. mutans

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The effect of CP on glycolytic acid production by S. mutans planktonic cells was measured, as described elsewhere [33 (link)]. Briefly, S. mutans or L. rhamnosus cells in the planktonic cultures were harvested, washed once with a salt solution (50 mM KCl + 1 mM MgCl₂, pH = 7), and resuspended in a salt solution. The pH was adjusted to 7.0 with 0.2 M KOH solution. Glucose was then added to obtain a concentration of 1% (w/v), and pH change was assessed over a period of 90 min using a glass electrode (Orion 3-Star, Thermo Scientific, Waltham, MA, USA). The initial rate of acid production, which provides the best measure of the acid production capacity of the cells, was calculated using pH values.
H-Gly-Pro-Hyp-OH (Bachem, Bubendorf, Switzerland), a common tripeptide unit of collagen, was used for testing. Glycine (Shijiazhuang Shixing Amino Acid Co., Ltd., Shijiazhuang, China), one of most abundant amino acids in CP, was also used to compare the activity of CP. H-Gly-Pro-Hyp-OH, glycine, and CP were used at 5 mg/mL (0.5% (w/v)).

Jung H.Y., Cai J.N., Yoo S.C., Kim S.H., Jeon J.G, & Kim D. (2022). Collagen Peptide in a Combinatorial Treatment with Lactobacillus rhamnosus Inhibits the Cariogenic Properties of Streptococcus mutans: An In Vitro Study. International Journal of Molecular Sciences, 23(3), 1860.

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Rapid Microextraction and Smartphone-Based Analysis

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Using the results of our multivariate analysis, our final DLLME protocol calls for a mixture of 80 μg mL⁻¹ of IBU, 5% (w v⁻¹) of cobalt salt and 5% (w v⁻¹) of NaCl solutions mixed well in a15 mL test tube, with a pH corrected to 7 and a final volume adjusted to 10 mL. pH measurements were performed with a pH meter (model Orion 3 Star, Thermo Scientific, Waltham, MA, USA). Then, a mixture of 99 μL of extractant solvent (i.e., chloroform) and 319 μL of dispersant volume (methanol) are added using a syringe. A cloudy solution immediately forms and the phase separation is allowed to proceed for one minute. Chloroform was chosen over non-toxic solutions (e.g., undecanol) as it allows phase separation to occur without centrifugation, allowing for a truly portable sample preparation. Afterwards, the aqueous phase is removed and the organic phase is retrieved with a pipette and analyzed by the smartphone-based system (Fig. 1). A novel component of this work is the direct measurement of the organic phase using a custom cartridge that integrates a linear series of fluid compartments made of polyoxymethylene (POM), which has excellent chemical resistance to most organic solvents (Fig 1B and 1C). From beginning to end, the overall procedure lasts less than 5 minutes.

Aguirre M.Á., Long K.D, & Cunningham B.T. (2019). Spectrometric smartphone-based system for ibuprofen quantification in commercial dosage tablets. Journal of pharmaceutical sciences, 108(8), 2593-2598.

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Quantifying Metal Concentrations

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Total dissolved metal concentrations were measured using either inductively coupled plasma optical emission spectrometry (ICP‐OES) (Perkin Elmer Optima 5300‐DV) or inductively coupled plasma mass spectrometry (ICP‐MS) (Agilent‐7700). Solution pH was monitored using a pH metre (Orion 3 star, Thermo).

Rushworth D.D., Christl I., Kumar N., Hoffmann K., Kretzschmar R., Lehmann M.F., Schenkeveld W.D, & Kraemer S.M. (2022). Copper mobilisation from Cu sulphide minerals by methanobactin: Effect of pH, oxygen and natural organic matter. Geobiology, 20(5), 690-706.

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Analytical Balance and pH Meter Calibration

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pH readings were obtained by an Orion 3 star (Thermo Scientific, Waltham, MA, USA) pH meter equipped with either a Thermo pH electrode (9142BN) or an Orion 8103BNUWP Ross Ultra Semi-micro pH probe (Thermo Scientific, USA) filled with 3M KCl ROSS Orion filling solution (Thermo Scientific, USA). An Ohaus ADVENTURER AX124 analytical balance (Ohaus, Parsippany, NJ, USA) was used for mass measurements. Samples were weighed on 3 × 3 inch low-nitrogen-weighing Fisherbrand paper (Fisherbrand, Pittsburgh, PA, USA). In general, a mass of at least 5 mg was weighed for all samples to minimize error. Both the balance and pH meter were calibrated directly prior to use. Balance calibration was confirmed with a 5 mg standard weight (Troemner, Thorofare, NJ, USA) with 5 ± 0.1 mg cutoff.

Wallach J., Gamrat J., Jauhola-Straight R., Becker J, & Eckrich T. (2022). Three Birds, One Excipient: Development of an Improved pH, Isotonic, and Buffered Ketamine Formulation for Subcutaneous Injection. Pharmaceutics, 14(3), 556.

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pH Measurement of MTA and α-TCP

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Specimens (1-mm thickness and 5-mm diameter) of MTA and α-TCP were prepared and allowed to set for 1 day (n = 3). After setting, one tablet was inserted into 10 mL of deionized water. Then, the resultant pH value was measured using a pH meter (Orion 3 Star; Thermo Scientific, Singapore). The apparatus was previously calibrated with pH 7.0 and pH 4.0 solutions. Between each measurement the electrode was washed with ultrapure water and blot-dried.

Lee J.B., Park S.J., Kim H.H., Kwon Y.S., Lee K.W, & Min K.S. (2014). Physical properties and biological/odontogenic effects of an experimentally developed fast-setting α-tricalcium phosphate-based pulp capping material. BMC Oral Health, 14, 87.

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