Polyclonal anti-CTGF (diluted at 1:1000) and monoclonal anti-α-tubulin (diluted at 1:5000) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Polyclonal anti-Smad4 (diluted at 1:1000), anti-TGF-β receptor type I (diluted at 1:1000), anti-ERK1/2 (diluted at 1:2000), monoclonal anti-Smad2 (diluted at 1:1000) and anti-Smad3 (diluted at 1:1000) antibodies were obtained from Cell Signaling Technology (Danvers, MA). Horseradish peroxidase-conjugated goat anti-mouse and goat anti-rabbit IgG (diluted at 1:5000) were obtained from Bio-Rad Laboratories (Hercules, CA), and horseradish peroxidase-conjugated donkey anti-goat IgG (diluted at 1:5000) was obtained from Santa Cruz Biotechnology. Recombinant human TGF-β 1 was obtained from R&D systems (Minneapolis, MN). SB431542 was obtained from Sigma-Aldrich (Oakville, ON), and U0126 was obtained from Calbiochem (San Diego, CA).
Horseradish peroxidase conjugated goat anti mouse and goat anti rabbit igg
Manufactured by Bio-Rad
Horseradish peroxidase-conjugated goat anti-mouse and goat anti-rabbit IgG are secondary antibodies used as detection reagents in various immunoassay techniques, such as Western blotting, ELISA, and immunohistochemistry. These antibodies are conjugated with the enzyme horseradish peroxidase, which catalyzes a colorimetric or chemiluminescent reaction when exposed to the appropriate substrate, allowing for the visualization and quantification of target proteins.
Automatically generated - may contain errors
Lab products found in correlation
Horseradish peroxidase conjugated donkey anti goat igg, by Santa Cruz Biotechnology (2 mentions)
Monoclonal anti α tubulin, by Santa Cruz Biotechnology (1 mentions)
Recombinant human tgf β1, by R&D Systems (1 mentions)
Sb431542, by Merck Group (1 mentions)
Anti smad3, by Cell Signaling Technology (1 mentions)
Anti erk1 2, by Cell Signaling Technology (1 mentions)
Sew2871, by Bio-Techne (1 mentions)
Cym5541, by Bio-Techne (1 mentions)
Cay10444, by Cayman Chemical (1 mentions)
Monoclonal anti α tubulin sc 23948, by Santa Cruz Biotechnology (1 mentions)
Sphingosine 1 phosphate (s1p), by Avanti Polar Lipids (1 mentions)
2 protocols using horseradish peroxidase conjugated goat anti mouse and goat anti rabbit igg
1
TGF-β Signaling Pathway Analysis
2
Sphingosine-1-Phosphate Signaling Pathway Analysis
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Polyclonal anti-COX-1 (#sc-1752) and monoclonal anti-α-tubulin (#sc-23948) antibodies were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Polyclonal anti-COX-2 (#ab52237) and monoclonal anti-S1P 1 (#ab125074) and anti-S1P 3 (#ab108370) antibodies were obtained from Abcam (Cambridge, MA). Monoclonal anti-phospho-YAP ser127 (#13008) and anti-YAP (#12395) antibodies were obtained from Cell Signaling Technology (Danvers, MA). Horseradish peroxidase-conjugated goat anti-mouse and goat anti-rabbit IgG were obtained from Bio-Rad Laboratories (Hercules, CA). Horseradish peroxidaseconjugated donkey anti-goat IgG was obtained from Santa Cruz Biotechnology. Sphingosine-1-phosphate was obtained from Avanti Polar Lipids (Alabaster, AL) and prepared in PET solution (5% polyethylene glycol, 2.5% ethanol and 0.8% Tween-80) [31] . W146 and CAY10444 were obtained from Cayman Chemical (Ann Arbor, MI). S1P 1 agonist SEW 2871 and S1P 3 agonist CYM 5541 were obtained from Tocris (Minneapolis, MN).
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