Dii 1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanineperchlorate

Manufactured by Thermo Fisher Scientific

DiI (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanineperchlorate) is a lipophilic carbocyanine dye used for fluorescent labeling. It is a long-chain dialkylcarbocyanine compound that can be incorporated into the lipid bilayers of cell membranes.

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Lab products found in correlation

Milli q device, by Merck Group (1 mentions) Egg phosphatidylcholine epc, by Lipoid (1 mentions) Sephacryls500 hr, by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Ethanol, by Carl Roth (1 mentions)

Close spelling variants of this product

1,1-dioctadecyl-3

2 protocols using dii 1 1 dioctadecyl 3 3 3 3 tetramethylindocarbocyanineperchlorate

Liposome Formulation and Characterization

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Phosphate-buffered
saline (PBS) and Sephacryl
S500-HR were procured from Sigma-Aldrich (USA), whereas ethanol (99.5%)
and cholesterol (Chol) were acquired from Carl Roth, Germany. A Milli
Q device (Merck Millipore, Germany) was used to obtain demineralized
water. Egg phosphatidyl choline (EPC) and mPEG-DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene
glycol)-3000], both from Lipoid, Germany) were used for liposome formulation.
The dialkyl-based hbPG amphiphiles were synthesized
as described in the Supporting Information,^{33 (link)} where also the polymer characterization
can be found. The membrane dye DiI (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine
perchlorate) was purchased from Thermo Fisher Scientific.

Weber C., Voigt M., Simon J., Danner A.K., Frey H., Mailänder V., Helm M., Morsbach S, & Landfester K. (2019). Functionalization of Liposomes with Hydrophilic Polymers Results in Macrophage Uptake Independent of the Protein Corona. Biomacromolecules, 20(8), 2989-2999.

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Exosomes Targeted to the CNS

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The exosomes were tagged with RVG peptide (YTIWMPENPRPGTPCDIFTNSRGKRASNG) shown to target the CNS. The peptide was custom synthesized and then was conjugated to the exosomes using modifications of previously described methods [16 (link)]. DOPE-NHS (dioleoylphosphatidylethanolamine N-hydroxysuccinimide; COATSOME® FE-8181SU5, NOF America; White Plains, NY) and RVG peptide were combined and allowed to react for 1 h to obtain the DOPE-RVG. The DOPE-RVG was then incubated with the exosomes derived from MSCs. The DOPE-RVG to exosome labelling ratio was determined as 500:1 considering the length of peptide and radius of exosome. The modified exosomes subsequently were labelled with lipophilic dye DiI (1,1′-Dioctadecyl-3,3,3′,3′-Tetramethylindocarbocyanine Perchlorate, ThermoFisher Scientific, Waltham, MA) for the biodistribution detection.

Cui G.H., Guo H.D., Li H., Zhai Y., Gong Z.B., Wu J., Liu J.S., Dong Y.R., Hou S.X, & Liu J.R. (2019). RVG-modified exosomes derived from mesenchymal stem cells rescue memory deficits by regulating inflammatory responses in a mouse model of Alzheimer’s disease. Immunity & Ageing : I & A, 16, 10.

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