Antibodies against mcl 1

Manufactured by Santa Cruz Biotechnology

Antibodies against MCL-1 are a product offered by Santa Cruz Biotechnology. MCL-1 is a protein that plays a role in cell survival and apoptosis. These antibodies can be used to detect and study the expression and function of MCL-1 in various biological samples.

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Lab products found in correlation

Odyssey software, by LI COR (1 mentions) P stat5, by Cell Signaling Technology (1 mentions) Stat5, by Cell Signaling Technology (1 mentions) Theodyssey infrared imaging system, by LI COR (1 mentions) 4e bp1, by Cell Signaling Technology (1 mentions) P akt, by Cell Signaling Technology (1 mentions) Bcl xl, by Cell Signaling Technology (1 mentions) P 4ebp1, by Cell Signaling Technology (1 mentions) P erk, by Cell Signaling Technology (1 mentions) Enhanced chemiluminescence detection, by GE Healthcare (1 mentions) Polyvinylidene fluoride transfer membranes, by GE Healthcare (1 mentions)

Close spelling variants of this product

Mcl-1 (192 citations)

2 protocols using antibodies against mcl 1

Quantitative Western Blot Analysis of Signaling Pathways

Cited 1 time

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Western blot was performed as previously described (4 (link)). Antibodies against FAK (cat#13009s), p-STAT5
(cat#9351s), STAT5 (cat#9363s), p-AKT (cat#4060s), AKT (cat#2920s), BCL-XL
(cat#2764s), p-ERK (cat#4370s), 4EBP1 (cat#9644), and p-4EBP1 (cat#2855) were
purchased from Cell Signaling Technology (Danvers, MA). Antibodies against MCL-1
(cat#sc-819) and BCL-2 (cat#M0887) were purchased from Santa Cruz Biotechnology
(Santa Cruz, CA) and DAKO (Carpentaria, CA), respectively. β-actin or
α-tubulin was used as a loading control. Signals were detected using the
Odyssey Infrared Imaging System (LI-COR Biosciences, Lincoln, NE) and quantified
using Odyssey software (version 3.0, LI-COR Biosciences).

Wang X., Mak P.Y., Mu H., Tao W., Rao A., Visweswaran R., Ruvolo V., Pachter J.A., Weaver D.T., Andreeff M., Xu B, & Carter B.Z. (2020). Combinatorial inhibition of focal adhesion kinase and BCL-2 enhances anti-leukemia activity of venetoclax in acute myeloid leukemia. Molecular cancer therapeutics, 19(8), 1636-1648.

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Immunoblot Analysis of MCL-1 and pSer2

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For each sample, the total protein was separated by 8% or 10% sodium dodecyl
sulfate–polyacrylamide gel electrophoresis and transferred onto polyvinylidene
fluoride transfer membranes (GE Healthcare Life Sciences, Pittsburgh, PA).
Antibodies against MCL-1 (Santa Cruz Biotechnology, Santa Cruz, CA) and
phosphorylated RNA polymerase II CTD (pSer2; Novus Biologicals, Littleton, CO)
were used for immunoblotting of MCL-1 and pSer2 proteins. Bands were visualized
by enhanced chemiluminescence detection (GE Healthcare Life Sciences). The
experiments were performed in triplicate for each treatment condition.

Tong Z., Mejia A., Veeranki O., Verma A., Correa A.M., Dokey R., Patel V., Solis L.M., Mino B., Kathkuda R., Rodriguez-Canales J., Lin S.H., Krishnan S., Kopetz S., Blum M., Ajani J.A., Hofstetter W.L, & Maru D.M. (2019). Targeting CDK9 and MCL-1 by a new CDK9/p-TEFb inhibitor with and without 5-fluorouracil in esophageal adenocarcinoma. Therapeutic Advances in Medical Oncology, 11, 1758835919864850.

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